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- PDB-6ymi: Crystal structure of the SAM-SAH riboswitch with AMP. -

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Basic information

Entry
Database: PDB / ID: 6ymi
TitleCrystal structure of the SAM-SAH riboswitch with AMP.
Components
  • Chains: A,C,F,I,M,O
  • Chains: B,D,G,J,N,P
KeywordsRNA / Pseudoknot / SAM / Riboswitch
Function / homologyADENOSINE MONOPHOSPHATE / 5-BROMOCYTIDINE 5'-(DIHYDROGEN PHOSPHATE) / RNA / RNA (> 10)
Function and homology information
Biological speciesRoseobacter sp. (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å
AuthorsHuang, L. / Lilley, D.M.J.
Funding support United Kingdom, China, 2items
OrganizationGrant numberCountry
Cancer Research UKA18604 United Kingdom
Ministry of Education (MoE, China) China
CitationJournal: Nucleic Acids Res. / Year: 2020
Title: Crystal structure and ligand-induced folding of the SAM/SAH riboswitch.
Authors: Huang, L. / Liao, T.W. / Wang, J. / Ha, T. / Lilley, D.M.J.
History
DepositionApr 8, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 22, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 2.0Apr 20, 2022Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Database references / Derived calculations / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / database_2 / entity / ndb_struct_na_base_pair / ndb_struct_na_base_pair_step / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_struct_assembly_gen / pdbx_unobs_or_zero_occ_residues / pdbx_validate_close_contact / pdbx_validate_rmsd_angle / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.group_PDB / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.label_seq_id / _atom_site.type_symbol / _atom_site_anisotrop.id / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _entity.pdbx_number_of_molecules / _pdbx_poly_seq_scheme.auth_mon_id / _pdbx_poly_seq_scheme.auth_seq_num / _pdbx_poly_seq_scheme.pdb_mon_id / _pdbx_poly_seq_scheme.pdb_seq_num / _pdbx_struct_assembly_gen.asym_id_list / _struct_site.details / _struct_site.pdbx_auth_seq_id / _struct_site_gen.auth_seq_id / _struct_site_gen.label_asym_id
Revision 2.1Mar 8, 2023Group: Structure summary / Category: struct / Item: _struct.title
Revision 2.2Feb 7, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Chains: A,C,F,I,M,O
B: Chains: B,D,G,J,N,P
C: Chains: A,C,F,I,M,O
D: Chains: B,D,G,J,N,P
F: Chains: A,C,F,I,M,O
G: Chains: B,D,G,J,N,P
I: Chains: A,C,F,I,M,O
J: Chains: B,D,G,J,N,P
M: Chains: A,C,F,I,M,O
N: Chains: B,D,G,J,N,P
O: Chains: A,C,F,I,M,O
P: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,84926
Polymers67,71412
Non-polymers5,13514
Water36020
1
A: Chains: A,C,F,I,M,O
B: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,3825
Polymers11,2862
Non-polymers1,0973
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Chains: A,C,F,I,M,O
D: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,0354
Polymers11,2862
Non-polymers7492
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
F: Chains: A,C,F,I,M,O
G: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,3825
Polymers11,2862
Non-polymers1,0973
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
I: Chains: A,C,F,I,M,O
J: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,0354
Polymers11,2862
Non-polymers7492
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
M: Chains: A,C,F,I,M,O
N: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)12,3825
Polymers11,2862
Non-polymers1,0973
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
O: Chains: A,C,F,I,M,O
P: Chains: B,D,G,J,N,P
hetero molecules


Theoretical massNumber of molelcules
Total (without water)11,6333
Polymers11,2862
Non-polymers3471
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)86.506, 147.359, 74.844
Angle α, β, γ (deg.)90.000, 91.364, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61
12
22
32
42
52
62

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GGCCchain 'A'AA7 - 321 - 26
211GGCCchain 'C'CC7 - 321 - 26
311GGCCchain 'F'FE7 - 321 - 26
411GGCCchain 'I'IG7 - 321 - 26
511GGCCchain 'M'MI7 - 321 - 26
611GGCCchain 'O'OK7 - 321 - 26
122AAAAchain 'B'BB43 - 511 - 9
222AAAAchain 'D'DD43 - 511 - 9
322AAAAchain 'G'GF43 - 511 - 9
422AAAAchain 'J'JH43 - 511 - 9
522AAAAchain 'N'NJ43 - 511 - 9
622AAAAchain 'P'PL43 - 511 - 9

NCS ensembles :
ID
1
2

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Components

#1: RNA chain
Chains: A,C,F,I,M,O


Mass: 8389.874 Da / Num. of mol.: 6 / Source method: obtained synthetically / Source: (synth.) Roseobacter sp. (bacteria)
#2: RNA chain
Chains: B,D,G,J,N,P


Mass: 2895.791 Da / Num. of mol.: 6 / Source method: obtained synthetically / Source: (synth.) Roseobacter sp. (bacteria)
#3: Chemical
ChemComp-CBV / 5-BROMOCYTIDINE 5'-(DIHYDROGEN PHOSPHATE)


Type: RNA linking / Mass: 402.093 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C9H13BrN3O8P
#4: Chemical
ChemComp-AMP / ADENOSINE MONOPHOSPHATE / Adenosine monophosphate


Mass: 347.221 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: C10H14N5O7P / Feature type: SUBJECT OF INVESTIGATION / Comment: AMP*YM
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 20 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.55 Å3/Da / Density % sol: 65.32 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9119 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 2, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9119 Å / Relative weight: 1
ReflectionResolution: 2.5→74.82 Å / Num. obs: 57583 / % possible obs: 99.2 % / Redundancy: 3.4 % / Biso Wilson estimate: 60.27 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.111 / Rpim(I) all: 0.072 / Net I/σ(I): 8.3
Reflection shellResolution: 2.5→2.54 Å / Rmerge(I) obs: 0.83 / Num. unique obs: 1582 / CC1/2: 0.55 / Rpim(I) all: 0.52 / % possible all: 98.6

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
PHENIX1.17.1_3660refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6YL5

6yl5


Resolution: 2.5→74.59 Å / SU ML: 0.5026 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 27.0383
RfactorNum. reflection% reflection
Rfree0.2418 2895 5.03 %
Rwork0.2138 --
obs0.2151 57583 90.06 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 69.82 Å2
Refinement stepCycle: LAST / Resolution: 2.5→74.59 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms0 4326 333 20 4679
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00255181
X-RAY DIFFRACTIONf_angle_d0.79738022
X-RAY DIFFRACTIONf_chiral_restr0.03621068
X-RAY DIFFRACTIONf_plane_restr0.0058219
X-RAY DIFFRACTIONf_dihedral_angle_d15.26292517
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5-2.540.45811160.3911982X-RAY DIFFRACTION69.42
2.54-2.580.39491510.37672507X-RAY DIFFRACTION86.27
2.58-2.630.4341480.36442477X-RAY DIFFRACTION87.06
2.63-2.680.37271550.33912619X-RAY DIFFRACTION90.06
2.68-2.740.31981900.32442491X-RAY DIFFRACTION90.42
2.74-2.80.34251560.3272618X-RAY DIFFRACTION90.51
2.8-2.860.33781360.31982605X-RAY DIFFRACTION89.46
2.86-2.930.35541340.31382610X-RAY DIFFRACTION90.2
2.93-3.010.31421620.28472548X-RAY DIFFRACTION88.36
3.01-3.10.27061240.26942538X-RAY DIFFRACTION88.12
3.1-3.20.31961670.23772575X-RAY DIFFRACTION90.79
3.2-3.320.26791590.22982724X-RAY DIFFRACTION93.39
3.32-3.450.24451000.21262730X-RAY DIFFRACTION93.09
3.45-3.610.24491420.2172693X-RAY DIFFRACTION93.16
3.61-3.80.21841360.18542654X-RAY DIFFRACTION92.14
3.8-4.030.2027910.18432750X-RAY DIFFRACTION92.24
4.03-4.340.20571490.17292584X-RAY DIFFRACTION90.29
4.35-4.780.2177850.18082708X-RAY DIFFRACTION91.88
4.78-5.470.21561600.17712722X-RAY DIFFRACTION94.77
5.48-6.90.1852990.17182772X-RAY DIFFRACTION93.88
6.9-74.590.13861350.17292781X-RAY DIFFRACTION95.54
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.5669758993350.587881085306-0.1693021658381.770248019470.6406322400320.240574242444-0.08976683702580.05480338420330.540560277538-0.6180386141440.0006609727117520.637357904907-0.22252228355-0.2010743332-0.0003793382977210.6197422198890.0614036410971-0.08227112530470.6328522186430.05828613129030.59514070903730.741844365413.160779982922.5402790214
20.5409288422580.1387288050990.1431674524220.1681164163680.2181702909310.204724171434-0.00986980964455-0.22143776255-0.748463889044-0.114484123898-0.266002968277-0.303219478241-0.113188021991-0.1514996774715.53802965394E-50.539792126466-0.05090598061470.1177352065190.641868796491-0.01050478869680.61721999598944.431610507112.043961354430.2730535148
30.3959996880480.06132231011080.01381225655442.0399583948-0.8218061453970.303872389101-0.178461752023-0.09094656398920.108299541627-0.6218237819450.1424964515780.702994446487-0.216429032641-0.157243710823-0.00134599732530.493416688524-0.00276875282823-0.04465676036010.6804687385980.0732544265690.55174906134825.478239845511.927122054121.1116395324
40.0648407970398-0.181412784428-0.004780324182290.7647041039090.4463211684840.1782577090250.2173838304380.4161286645170.3708699709320.107302897078-0.0003259104048870.116697706424-0.7396295692350.5389703183660.001165023605210.599580162343-0.01797292704090.0380320857320.6422659239720.01392937786640.65126331318845.393004883823.026383083327.5751160942
5-0.01083726536990.304999594823-0.1145127729041.03677236793-1.183961567061.17262659227-1.097628121860.469180028141.27043772693-0.942803550738-0.520656192870.0813521113362-0.09301075885440.281379773679-0.311823719480.374855155814-0.121303534854-0.2311465736670.94557620409-0.04543968580591.341868016-4.45461963732.9619072429810.6291339442
60.8910824611310.944906294540.8702412620792.20980909175-0.1918346071120.492809492173-0.9429044099730.2691082092940.276212085334-0.5646422459180.252378444722-0.658422600176-0.5319709504460.194356293185-0.009059443675680.742043134463-0.176656942734-0.1001748948730.889861403918-0.07879760520940.872616314806-5.290321807753.9771491945410.8402897164
70.5520396479020.0762046947222-1.119710806162.43626448241-1.137265963122.6334351477-0.4036786897490.3365953797570.330348483151-0.549565278490.159751682565-0.9916967844490.5684283810830.576183093174-0.0009547140740180.742808951337-0.08239009983090.02747901576930.862906840791-0.05890306557880.672808937121-14.0777288545-10.646880268910.9020776158
82.136681046370.8604845436730.204207338180.508780608152-0.3399843172340.281552560143-0.2382191908360.214587261875-0.390663071425-0.2325209307840.222994350163-0.1585979716770.264747090112-0.07668756875561.99794890952E-50.6249028246170.02606133815690.01682114855870.578879296586-0.09809980155670.449097962705-5.5539480863457.391402804722.5401787257
92.47302168285-0.8348851134760.8348276442320.380632891215-0.1854866870240.254924131525-0.000458314655777-0.143046568602-0.0811195427780.05261061917820.166096589005-0.0114556693227-0.0754841327971-0.01118981147930.0009984025735720.5697333603060.0557133365032-0.00126844954770.5403851358770.0239990179220.423904698171-10.751652681262.689878762326.0885774667
101.220743948410.4508375695120.1518483286210.1646853647930.07453277428680.568411163255-0.4374274177390.42781581874-1.517499868080.3074923685870.7554037652330.0172879795309-0.1151034746030.2255931962250.001941335099260.681937731650.146071210424-0.03598663814690.6331075329-0.1889774602290.6712807146996.5938073900350.896076878319.9684341204
110.891874348169-0.419075974103-0.4601663194880.992430637684-0.7539800928271.275429746230.176023063146-0.0276959422153-0.112141115618-0.339540183491-0.3346687167810.400090985103-0.393632945594-1.146989367660.0004371252933240.6016038999470.05806457411140.02453636100850.5733108380050.02069471407640.54826537834-21.630180360565.280676743727.0953758433
120.1768350269550.2188154903-0.1497564561670.4360296948350.1876469314380.617984094812-0.2360220186610.5093207845480.008322277453820.05524004188950.094338785424-1.053217443881.24356667087-0.6942345773440.01573848904991.002967090630.2185995989010.1198907311410.844167299053-0.3303819036631.2793369759612.346997726432.367819667316.224355636
131.267625348220.5904472767970.5240851283420.3231233779160.5917294003481.1549519398-0.2671931888091.063355829410.526439329309-0.06138561206640.151611090980.944564108388-0.242175487968-0.289315732231-0.001857589215370.8296734305630.189803042750.007279155343390.8236364292570.2535897289850.94727726038331.176810853128.49695598774.56783047508
140.382845011494-0.260456959207-0.4934126306770.1549106073590.3027924781280.379369477232-0.04109642484360.6909047153351.54659469709-0.265232174861-0.6318488359020.2930765958580.459254641155-0.498115408765-0.01326942031570.8120256234810.222799892668-0.03969761140220.918930782546-0.1018891660081.4248342235115.557375341235.323782334610.9273592337
152.513070047641.61932902091.339089491681.381990238130.08010075110111.96631228525-0.03754806466210.5496938611660.953607105139-0.7779531069230.3488248879030.997265772189-0.3478826985660.3366135740040.0007645191669850.7778315654850.147880238263-0.06328004899940.7612844827710.1948979660720.79155118506738.036991659731.36153535789.79096055217
161.48499975578-1.351589365830.07088905514291.17374129220.2691170023290.5327993907830.2748486708950.3462739714240.368560231593-0.0116688914526-0.491283766850.0661576751519-0.06821356756990.269992230715-0.0005081074488060.58660901306-0.04059137878950.101345953320.6426480197240.1058890947090.411119104095-26.84992931924.4542945452823.0215458527
170.5035540036460.2067570218940.2829327462340.714141685356-0.4523585965320.604723955835-0.0585209238062-0.03513323791210.262240478663-0.185035086082-0.1734986359640.616573751511-0.337468950173-0.1584627279440.0002226710530540.550434454864-0.02317336307060.02565810866970.536195549509-0.04747597972560.514267284304-34.9964424031-7.0178601122630.4170211351
182.03563241384-1.18170162311-0.6444786064580.6294595899360.3841996783780.1520729916770.1836028951390.24669807540.602445749182-0.220372842943-0.392373347143-0.124606859684-0.289367483910.108505481431-0.02447952233720.740906513347-0.1747404765220.09570669369830.631232442348-0.03643167259510.452024839405-25.27539490369.6385451980821.7656309658
191.125432772810.1973427618911.260301622310.9208528000580.1343651455831.2642613045-0.2097237776080.1693015371010.0346268313483-0.3211953840970.3277081291750.02702833753960.6800996528460.2749640150210.000114503165410.556194403444-0.06465866923460.0178048655530.594625157833-0.04418237179960.48108611872-25.7679806171-13.264389706427.9966638702
200.4187536032940.109886013287-0.01190160758040.0407989571801-0.1675397581570.8267637767370.7097344460930.01827405527920.6165755550320.257908820619-0.9859407271940.249633299423-0.4858104970941.466726633220.02990619430951.0397853168-0.1641807327150.09246835141570.7434690606860.3040481613841.66506518721-13.30367937531.967691043616.6108570433
210.988725221171-0.809346927813-1.022768002021.032116579880.2338060040921.485106521050.2856223840060.0611357889865-1.03820827597-0.354304447513-0.479548337068-0.01318361805570.276838400432-0.459986780896-0.0009236434191110.814339208941-0.0702476627008-0.1655834133090.619203247189-0.05114601463430.819901867909-18.698146360749.81310850974.41403304936
220.107004623660.11525715357-0.3854321578280.123274444858-0.4496061664150.960957363769-0.220661659214-0.114239189141-0.653714126393-0.663923708978-0.3795771421180.4537090944280.5521008135410.689830834849-0.0003030132331861.01261481039-0.06532169111720.04461178533780.6013314009330.05058335672881.37488852952-17.230693355533.081037110711.1206302829
231.26532152542-1.24630590312-1.464754171211.274787587741.624899839712.65509919301-0.03564211319370.374071125991-0.936019005906-0.804094728804-0.2791422794920.921853028580.0635022903689-0.745747771709-0.0007188228836740.711288715831-0.0409840584917-0.2129606898280.662791135594-0.08709542938850.836297248166-24.785813939354.45518151469.34267934976
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 7 through 17 )
2X-RAY DIFFRACTION2chain 'A' and (resid 18 through 22 )
3X-RAY DIFFRACTION3chain 'A' and (resid 23 through 32 )
4X-RAY DIFFRACTION4chain 'B' and (resid 43 through 51 )
5X-RAY DIFFRACTION5chain 'C' and (resid 7 through 17 )
6X-RAY DIFFRACTION6chain 'C' and (resid 18 through 32 )
7X-RAY DIFFRACTION7chain 'D' and (resid 43 through 51 )
8X-RAY DIFFRACTION8chain 'F' and (resid 7 through 17 )
9X-RAY DIFFRACTION9chain 'F' and (resid 18 through 27 )
10X-RAY DIFFRACTION10chain 'F' and (resid 28 through 32 )
11X-RAY DIFFRACTION11chain 'G' and (resid 43 through 51 )
12X-RAY DIFFRACTION12chain 'I' and (resid 7 through 12 )
13X-RAY DIFFRACTION13chain 'I' and (resid 13 through 22 )
14X-RAY DIFFRACTION14chain 'I' and (resid 23 through 32 )
15X-RAY DIFFRACTION15chain 'J' and (resid 43 through 51 )
16X-RAY DIFFRACTION16chain 'M' and (resid 7 through 17 )
17X-RAY DIFFRACTION17chain 'M' and (resid 18 through 22 )
18X-RAY DIFFRACTION18chain 'M' and (resid 23 through 32 )
19X-RAY DIFFRACTION19chain 'N' and (resid 43 through 51 )
20X-RAY DIFFRACTION20chain 'O' and (resid 7 through 12 )
21X-RAY DIFFRACTION21chain 'O' and (resid 13 through 22 )
22X-RAY DIFFRACTION22chain 'O' and (resid 23 through 32 )
23X-RAY DIFFRACTION23chain 'P' and (resid 43 through 51 )

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