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- PDB-6w89: Structure of DNMT3A (R882H) in complex with CGA DNA -

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Basic information

Entry
Database: PDB / ID: 6w89
TitleStructure of DNMT3A (R882H) in complex with CGA DNA
Components
  • (DNA (cytosine-5)-methyltransferase ...) x 2
  • CGA DNA (25-MER)
KeywordsTRANSFERASE/DNA / DNA methylation / DNMT3A(R882H) / AML / epigenetics / TRANSFERASE / TRANSFERASE-DNA complex
Function / homology
Function and homology information


retrotransposon silencing by heterochromatin formation / epigenetic programing of female pronucleus / chorionic trophoblast cell differentiation / positive regulation of cellular response to hypoxia / DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates / epigenetic programming of gene expression / cellular response to bisphenol A / protein-cysteine methyltransferase activity / genomic imprinting / DNA (cytosine-5-)-methyltransferase ...retrotransposon silencing by heterochromatin formation / epigenetic programing of female pronucleus / chorionic trophoblast cell differentiation / positive regulation of cellular response to hypoxia / DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates / epigenetic programming of gene expression / cellular response to bisphenol A / protein-cysteine methyltransferase activity / genomic imprinting / DNA (cytosine-5-)-methyltransferase / unmethylated CpG binding / DNA (cytosine-5-)-methyltransferase activity / autosome genomic imprinting / S-adenosylmethionine metabolic process / negative regulation of DNA methylation-dependent heterochromatin formation / SUMOylation of DNA methylation proteins / DNA methylation-dependent heterochromatin formation / ESC/E(Z) complex / XY body / cellular response to ethanol / response to vitamin A / negative regulation of gene expression via chromosomal CpG island methylation / DNA metabolic process / negative regulation of gene expression, epigenetic / response to ionizing radiation / hepatocyte apoptotic process / male meiosis I / chromosome, centromeric region / catalytic complex / heterochromatin / enzyme activator activity / DNA methylation / Transferases; Transferring one-carbon groups; Methyltransferases / PRC2 methylates histones and DNA / response to cocaine / condensed nuclear chromosome / Defective pyroptosis / stem cell differentiation / placenta development / cellular response to amino acid stimulus / response to lead ion / euchromatin / neuron differentiation / response to toxic substance / RMTs methylate histone arginines / nuclear matrix / transcription corepressor activity / response to estradiol / cellular response to hypoxia / methylation / spermatogenesis / RNA polymerase II-specific DNA-binding transcription factor binding / response to xenobiotic stimulus / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / chromatin binding / enzyme binding / negative regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
DNA (cytosine-5)-methyltransferase 3A, ADD domain / : / DNMT3, ADD PHD zinc finger / DNMT3, cysteine rich ADD domain / DNMT3, cysteine rich ADD domain, GATA1-like zinc finger / ADD domain / ADD domain profile. / DNA methylase, C-5 cytosine-specific, active site / C-5 cytosine-specific DNA methylases active site. / C-5 cytosine-specific DNA methylase (Dnmt) domain profile. ...DNA (cytosine-5)-methyltransferase 3A, ADD domain / : / DNMT3, ADD PHD zinc finger / DNMT3, cysteine rich ADD domain / DNMT3, cysteine rich ADD domain, GATA1-like zinc finger / ADD domain / ADD domain profile. / DNA methylase, C-5 cytosine-specific, active site / C-5 cytosine-specific DNA methylases active site. / C-5 cytosine-specific DNA methylase (Dnmt) domain profile. / C-5 cytosine methyltransferase / C-5 cytosine-specific DNA methylase / domain with conserved PWWP motif / PWWP domain / PWWP domain profile. / PWWP domain / Vaccinia Virus protein VP39 / Zinc finger, FYVE/PHD-type / Zinc finger, RING/FYVE/PHD-type / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
CITRIC ACID / S-ADENOSYL-L-HOMOCYSTEINE / DNA / DNA (> 10) / DNA (cytosine-5)-methyltransferase 3-like / DNA (cytosine-5)-methyltransferase 3A
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.499 Å
AuthorsAnteneh, H. / Song, J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R35GM119721 United States
CitationJournal: Nat Commun / Year: 2020
Title: Structural basis for impairment of DNA methylation by the DNMT3A R882H mutation.
Authors: Anteneh, H. / Fang, J. / Song, J.
History
DepositionMar 20, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 15, 2020Provider: repository / Type: Initial release
Revision 1.1May 20, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Oct 18, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA (cytosine-5)-methyltransferase 3A
B: DNA (cytosine-5)-methyltransferase 3-like
D: DNA (cytosine-5)-methyltransferase 3A
C: DNA (cytosine-5)-methyltransferase 3-like
E: CGA DNA (25-MER)
F: CGA DNA (25-MER)
G: DNA (cytosine-5)-methyltransferase 3A
H: DNA (cytosine-5)-methyltransferase 3-like
J: DNA (cytosine-5)-methyltransferase 3A
I: DNA (cytosine-5)-methyltransferase 3-like
K: CGA DNA (25-MER)
L: CGA DNA (25-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)260,54420
Polymers258,23712
Non-polymers2,3068
Water4,666259
1
A: DNA (cytosine-5)-methyltransferase 3A
B: DNA (cytosine-5)-methyltransferase 3-like
D: DNA (cytosine-5)-methyltransferase 3A
C: DNA (cytosine-5)-methyltransferase 3-like
E: CGA DNA (25-MER)
F: CGA DNA (25-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)130,27210
Polymers129,1196
Non-polymers1,1534
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: DNA (cytosine-5)-methyltransferase 3A
H: DNA (cytosine-5)-methyltransferase 3-like
J: DNA (cytosine-5)-methyltransferase 3A
I: DNA (cytosine-5)-methyltransferase 3-like
K: CGA DNA (25-MER)
L: CGA DNA (25-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)130,27210
Polymers129,1196
Non-polymers1,1534
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)187.559, 187.559, 82.398
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number145
Space group name H-MP32

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Components

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DNA (cytosine-5)-methyltransferase ... , 2 types, 8 molecules ADGJBCHI

#1: Protein
DNA (cytosine-5)-methyltransferase 3A / DNA (cytosine-5)-methyltransferase 3A / Dnmt3a / DNA methyltransferase HsaIIIA / M.HsaIIIA


Mass: 32696.668 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DNMT3A / Production host: Escherichia coli (E. coli)
References: UniProt: Q9Y6K1, DNA (cytosine-5-)-methyltransferase
#2: Protein
DNA (cytosine-5)-methyltransferase 3-like


Mass: 24163.705 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DNMT3L / Production host: Escherichia coli (E. coli) / References: UniProt: Q9UJW3

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DNA chain , 1 types, 4 molecules EFKL

#3: DNA chain
CGA DNA (25-MER)


Mass: 7698.969 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 3 types, 267 molecules

#4: Chemical
ChemComp-CIT / CITRIC ACID / Citric acid


Mass: 192.124 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H8O7
#5: Chemical
ChemComp-SAH / S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine


Type: L-peptide linking / Mass: 384.411 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C14H20N6O5S
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 259 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.24 Å3/Da / Density % sol: 62.09 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / Details: 0.1 M Sodium Citrate pH 4.2, 1% PEG8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jan 26, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.499→37.72 Å / Num. obs: 110518 / % possible obs: 98.5 % / Redundancy: 6.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.078 / Net I/σ(I): 10.4
Reflection shellResolution: 2.5→2.59 Å / Num. unique obs: 110518 / CC1/2: 0.726

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
PDB_EXTRACT3.25data extraction
HKL-3000data reduction
HKL-3000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5YX2

5yx2


Resolution: 2.499→37.72 Å / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 31.32 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2404 2010 1.82 %
Rwork0.196 108507 -
obs0.1968 110517 98.5 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 251.83 Å2 / Biso mean: 90.5492 Å2 / Biso min: 30 Å2
Refinement stepCycle: final / Resolution: 2.499→37.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms14612 2019 156 259 17046
Biso mean--73.97 68.76 -
Num. residues----1989
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.4993-2.56170.381320.3422728793
2.5617-2.6310.31351380.3105747895
2.631-2.70840.38241450.2969760197
2.7084-2.79580.33611370.2835773798
2.7958-2.89570.31591470.2592779699
2.8957-3.01160.28381480.23517910100
3.0116-3.14860.27521460.22417850100
3.1486-3.31450.29031390.21957831100
3.3145-3.5220.29181440.2081777099
3.522-3.79370.20081460.17917871100
3.7937-4.1750.2121440.17177855100
4.175-4.77810.18581400.14977850100
4.7781-6.0160.21891480.1727859100
6.016-37.720.21411560.1765781299
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.37341.22680.81025.2372-0.32933.17190.07030.0326-0.0401-0.1366-0.1220.30640.29220.07060.02940.4196-0.04480.13130.43130.00370.3228229.008-35.15311.6306
23.00631.33450.38311.81670.32362.17850.1335-0.56690.32220.7173-0.29080.73590.1887-0.34210.18210.6273-0.12880.29070.5956-0.06240.6587220.1151-33.35516.7138
34.37045.36970.17467.6219-1.09341.33190.3369-0.82420.45261.0356-0.43430.487-0.0391-0.15650.15620.5714-0.06430.16860.565-0.04480.3993238.2756-19.991120.6393
43.69551.92230.15435.2175-0.63852.1563-0.0187-0.1830.3740.00240.00230.29640.16620.02040.05410.3467-0.00660.08480.4449-0.03330.2892238.6309-21.643610.6216
59.63162.06640.7621.8174-0.18842.25120.1294-0.7109-0.3660.7971-0.41251.44160.4598-0.36990.18720.8103-0.24360.42491.018-0.08561.4968193.1271-49.7320.1827
65.7290.88841.94533.7723-1.54124.25750.2738-0.2298-0.10220.5534-0.12531.11240.1023-0.3702-0.05630.6682-0.16340.48830.7352-0.1381.2767201.5283-41.065616.9551
79.12732.74133.2010.93030.70781.4775-0.51240.74411.0281-0.11230.9030.5312-0.6348-0.7154-0.39130.6818-0.08020.24981.02810.08451.3238197.1854-34.0524.7272
83.4053-3.20372.32973.0415-2.13694.76990.41391.22070.6044-0.9210.51041.19120.3654-1.035-0.70620.6591-0.19620.18721.1940.06171.5635193.1052-40.76975.4419
99.3753.12344.42836.2752-1.92669.23140.53881.47292.342-0.61721.26381.06771.009-0.8295-1.46751.1043-0.020.14071.45790.61852.033187.3488-27.59623.7733
102.8521-0.3545-0.77894.3159-0.0313.1863-0.04481.1758-0.54230.14220.736-0.76980.2072-0.6989-0.340.7193-0.25690.13851.3608-0.05921.5411185.7372-47.962413.6128
115.50311.38120.23465.80170.82562.2575-0.0672-0.03910.25410.1111-0.0715-0.2679-0.02470.32410.11380.4038-0.0187-0.00470.45150.10210.3069263.0416-1.8730.555
122.26930.64021.00322.03391.01672.3189-0.32830.53330.7595-0.38660.07510.0762-0.37150.31850.28730.5244-0.1293-0.06820.60770.30840.6775260.58427.1575-14.3747
138.46384.3284-0.58114.79240.30732.522-0.47170.8320.2611-0.60840.24780.29930.12920.1140.19440.5383-0.0352-0.03990.50820.13610.3129247.2632-11.0203-18.2905
143.11821.91781.06174.10770.33653.2396-0.11540.17820.2509-0.2011-0.02280.37730.11920.08680.08860.39740.00170.01520.34570.07490.3355249.0386-11.3906-8.3202
152.52431.0130.61021.1550.6771.0053-0.52040.94450.5577-0.62020.34980.0192-0.88441.12520.1321.2671-0.9504-0.27341.14261.03512.4314276.457333.5694-18.2778
162.38340.2112-0.99214.3705-0.01434.798-0.09880.56181.3946-0.22540.0368-0.3835-1.36980.5538-0.01931.0889-0.33-0.170.81680.38861.5382266.990828.2988-13.5858
179.12462.01490.03923.88040.21794.04360.8716-0.81060.54481.6767-0.51920.3014-1.67140.4957-0.3771.8791-0.52030.14230.9986-0.00881.8571266.545839.2753-4.7012
184.83421.0231-4.61485.4275-2.436.6065-0.10470.15690.44720.25740.64251.6151-0.0823-1.3546-0.51550.64050.0869-0.05890.89770.31391.287232.3841.6164-10.4388
192.09373.5469-2.83477.5311-6.0286.31180.4284-0.9617-0.13550.9865-0.5970.7501-0.92790.1954-0.01391.3045-0.09930.5921.1578-0.21251.8058220.8668-10.282625.9369
206.02120.7942-0.53759.0877-6.95228.20560.3864-1.10390.64211.30390.22550.3343-1.965-0.31140.14770.9645-0.02010.42280.7093-0.31671.1608226.3392-14.413825.6535
211.35651.5897-1.95166.1425-5.66875.4910.38710.28970.76990.5105-0.02151.2499-1.6874-0.6062-0.59211.47710.23070.05220.8690.05731.653224.661-1.03284.2926
226.97263.1372-5.09975.0906-1.44963.8941-0.83811.2642-0.4511-0.60110.434-0.45570.3538-0.9887-0.06280.9622-0.0545-0.19910.98990.26051.528238.85575.8096-24.9642
233.7486-0.64360.32077.31470.58853.2983-0.02650.1805-0.3965-0.1321-0.03840.02030.120.24720.10750.46380.0140.05680.34990.10170.3396238.8015-90.036-12.1084
241.1949-0.37430.10173.85430.50922.3675-0.2913-0.411-0.49490.72220.05180.4850.42510.05550.25910.68030.06720.17860.48280.2210.6598232.8252-96.7532.944
251.9721-3.64430.42288.53450.12060.8647-0.2337-0.3694-0.08180.73790.19910.32090.11650.01440.03480.58810.00120.10970.51950.12210.3752232.6198-75.23166.5555
262.436-0.95670.57874.98480.24472.207-0.0642-0.26040.09360.4053-0.0390.56140.09970.04680.12770.481-0.01460.08970.39130.06340.3928229.9123-74.1593-1.5549
270.99721.20331.35577.9192-0.46182.8832-0.13360.1833-0.88550.45570.16690.29560.51630.2027-0.26491.14850.04280.37410.64490.24531.6465234.928-131.14692.5424
281.08442.75980.87949.19550.12942.6920.2767-0.554-1.03842.0377-0.33570.74650.364-0.2538-0.22721.42090.02510.36320.83740.37411.3562231.562-124.574512.3697
291.93621.21871.26086.9512-0.22444.1551-0.1006-0.1464-0.7638-0.17890.27860.73090.6192-0.3479-0.12980.8535-0.00630.32970.52960.24361.285227.8295-116.71850.0134
303.9436-1.58372.94144.5322-1.98964.10011.08771.5212-1.2961-2.29570.49780.98041.1357-0.0333-0.58491.5074-0.1125-0.30990.76010.06731.5293224.4574-125.0884-10.9343
313.8297-2.24122.61534.394-2.74833.2962-0.2526-1.0373-0.9484-1.13010.64691.93250.7378-1.2478-1.11351.0442-0.1722-0.22891.06190.48522.9219209.1796-121.2714-4.0947
322.4993-2.68531.01915.5585-2.13774.22710.0327-0.334-0.7468-1.02870.63320.06981.4341-0.3542-0.55461.3424-0.04170.10640.6324-0.01371.8714230.0615-134.8648-0.8296
334.7149-1.2345-0.27525.71861.16452.6624-0.0703-0.12250.40040.0747-0.04610.0262-0.31260.20440.14470.414-0.0174-0.08410.3570.05830.3251226.8838-43.8716-13.1652
341.2898-0.4807-0.18312.13160.68092.5025-0.12180.3120.3561-0.6048-0.10320.6578-0.4996-0.13330.32850.68060.0223-0.27340.43450.08820.7185217.8642-41.499-28.0999
351.606-1.1951-0.80958.35240.43051.871-0.02330.3971-0.0907-1.0075-0.17930.3028-0.07310.16110.1930.56610.0236-0.16060.51570.06420.339226.8859-62.1323-32.0067
362.8059-0.27190.28893.98131.47273.2392-0.03330.2009-0.1687-0.3522-0.07660.4130.06110.18230.02710.40760.0337-0.08320.420.05070.3634228.1875-61.0523-22.117
376.37834.63762.42714.97452.2892.60010.3621-0.32210.8378-1.4494-0.55010.5191-1.9307-0.91510.34882.30330.611-1.02990.34870.44412.3998200.752-12.2128-31.3363
381.525-1.3198-0.91253.6674-2.51034.9148-0.06040.69281.4859-0.50360.36851.4595-0.7547-0.797-0.23071.30020.2274-0.49450.7450.18031.6665205.0519-25.9846-33.0404
391.0913-0.0685-1.96489.38851.04813.97460.3012-0.24991.0790.1048-0.109-0.44-2.25140.37160.28742.0462-0.0128-0.59860.82910.01391.818215.8324-14.4219-24.7786
400.9817-1.4075-0.50072.9654-1.20474.3116-0.5553-0.78891.18710.59930.41951.2158-1.6057-1.62790.01061.12550.4414-0.30731.014-0.13221.8282198.2082-26.7436-18.2616
411.80321.9433-0.70014.49221.9352.7153-0.9742-1.0520.78680.04930.43311.6-0.8953-0.99020.34871.54470.58670.08881.30210.19062.034190.2277-21.1815-20.67
424.3703-1.6691-1.79497.0257-2.61438.46730.20480.5589-1.2396-1.0248-0.60990.70540.5751-0.8864-0.08690.72570.0316-0.39720.6478-0.05750.9148213.6563-60.6599-37.019
432.2754-0.83610.66934.5704-3.90013.92310.0927-1.0998-1.4453-0.28141.02241.42050.919-1.035-0.66760.8779-0.2553-0.19951.27290.35091.762205.5661-73.2331-16.5701
444.87860.3515-2.25670.93230.09146.4056-0.2562-0.4128-0.49321.69460.2889-0.1818-1.1665-0.6934-0.44171.3405-0.00230.45170.90210.42242.1631214.7181-84.765913.3737
455.9483-1.32696.33545.7093-3.41548.65170.2348-0.74150.4081.1654-0.04770.8695-0.6168-1.51350.23090.84190.01580.46290.78850.08381.1481219.5078-82.162512.0899
463.6928-1.94395.1313.882-4.8788.8377-0.435-0.1058-0.30230.05840.74991.4409-0.5865-1.8431-0.32930.82710.0308-0.09351.29410.05731.6268206.9965-76.6448-9.3835
474.7922-3.1803-2.91215.19-2.24127.32430.03980.38860.4655-1.52370.06650.61890.8299-0.1301-0.41960.92540.0381-0.4750.8371-0.11171.3899208.1642-60.8821-38.6635
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 628 through 698 )A628 - 698
2X-RAY DIFFRACTION2chain 'A' and (resid 699 through 784 )A699 - 784
3X-RAY DIFFRACTION3chain 'A' and (resid 785 through 849 )A785 - 849
4X-RAY DIFFRACTION4chain 'A' and (resid 850 through 912 )A850 - 912
5X-RAY DIFFRACTION5chain 'B' and (resid 178 through 228 )B178 - 228
6X-RAY DIFFRACTION6chain 'B' and (resid 229 through 291 )B229 - 291
7X-RAY DIFFRACTION7chain 'B' and (resid 292 through 318 )B292 - 318
8X-RAY DIFFRACTION8chain 'B' and (resid 319 through 332 )B319 - 332
9X-RAY DIFFRACTION9chain 'B' and (resid 333 through 353 )B333 - 353
10X-RAY DIFFRACTION10chain 'B' and (resid 354 through 380 )B354 - 380
11X-RAY DIFFRACTION11chain 'D' and (resid 628 through 698 )D628 - 698
12X-RAY DIFFRACTION12chain 'D' and (resid 699 through 784 )D699 - 784
13X-RAY DIFFRACTION13chain 'D' and (resid 785 through 849 )D785 - 849
14X-RAY DIFFRACTION14chain 'D' and (resid 850 through 912 )D850 - 912
15X-RAY DIFFRACTION15chain 'C' and (resid 185 through 199 )C185 - 199
16X-RAY DIFFRACTION16chain 'C' and (resid 200 through 324 )C200 - 324
17X-RAY DIFFRACTION17chain 'C' and (resid 325 through 378 )C325 - 378
18X-RAY DIFFRACTION18chain 'E' and (resid 423 through 438 )E423 - 438
19X-RAY DIFFRACTION19chain 'E' and (resid 439 through 446 )E439 - 446
20X-RAY DIFFRACTION20chain 'F' and (resid 423 through 428 )F423 - 428
21X-RAY DIFFRACTION21chain 'F' and (resid 429 through 438 )F429 - 438
22X-RAY DIFFRACTION22chain 'F' and (resid 439 through 447 )F439 - 447
23X-RAY DIFFRACTION23chain 'G' and (resid 629 through 698 )G629 - 698
24X-RAY DIFFRACTION24chain 'G' and (resid 699 through 784 )G699 - 784
25X-RAY DIFFRACTION25chain 'G' and (resid 785 through 839 )G785 - 839
26X-RAY DIFFRACTION26chain 'G' and (resid 840 through 912 )G840 - 912
27X-RAY DIFFRACTION27chain 'H' and (resid 178 through 206 )H178 - 206
28X-RAY DIFFRACTION28chain 'H' and (resid 207 through 228 )H207 - 228
29X-RAY DIFFRACTION29chain 'H' and (resid 229 through 319 )H229 - 319
30X-RAY DIFFRACTION30chain 'H' and (resid 320 through 339 )H320 - 339
31X-RAY DIFFRACTION31chain 'H' and (resid 340 through 360 )H340 - 360
32X-RAY DIFFRACTION32chain 'H' and (resid 361 through 380 )H361 - 380
33X-RAY DIFFRACTION33chain 'J' and (resid 628 through 698 )J628 - 698
34X-RAY DIFFRACTION34chain 'J' and (resid 699 through 784 )J699 - 784
35X-RAY DIFFRACTION35chain 'J' and (resid 785 through 849 )J785 - 849
36X-RAY DIFFRACTION36chain 'J' and (resid 850 through 912 )J850 - 912
37X-RAY DIFFRACTION37chain 'I' and (resid 184 through 206 )I184 - 206
38X-RAY DIFFRACTION38chain 'I' and (resid 207 through 270 )I207 - 270
39X-RAY DIFFRACTION39chain 'I' and (resid 271 through 280 )I271 - 280
40X-RAY DIFFRACTION40chain 'I' and (resid 281 through 332 )I281 - 332
41X-RAY DIFFRACTION41chain 'I' and (resid 333 through 378 )I333 - 378
42X-RAY DIFFRACTION42chain 'K' and (resid 423 through 428 )K423 - 428
43X-RAY DIFFRACTION43chain 'K' and (resid 429 through 438 )K429 - 438
44X-RAY DIFFRACTION44chain 'K' and (resid 439 through 447 )K439 - 447
45X-RAY DIFFRACTION45chain 'L' and (resid 423 through 428 )L423 - 428
46X-RAY DIFFRACTION46chain 'L' and (resid 429 through 438 )L429 - 438
47X-RAY DIFFRACTION47chain 'L' and (resid 439 through 447 )L439 - 447

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