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- PDB-6vm7: SILv44 T cell receptor bound to HLA-A2 presenting gp100 peptide (... -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6vm7
TitleSILv44 T cell receptor bound to HLA-A2 presenting gp100 peptide (ITDQVPFSV)
Components
  • (SILv44 T cell receptor ...) x 2
  • Beta-2-microglobulinBeta-2 microglobulin
  • MHC class I antigen, A-2 alpha chain
  • Melanocyte protein PMEL
KeywordsIMMUNE SYSTEM / TCR / MHC class I / HLA A2 / autoimmune / vitiligo / melanoma
Function / homology
Function and homology information


positive regulation of melanin biosynthetic process / cis-Golgi network membrane / melanin biosynthetic process / melanosome membrane / multivesicular body, internal vesicle / melanosome organization / multivesicular body membrane / antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib ...positive regulation of melanin biosynthetic process / cis-Golgi network membrane / melanin biosynthetic process / melanosome membrane / multivesicular body, internal vesicle / melanosome organization / multivesicular body membrane / antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / lumenal side of endoplasmic reticulum membrane / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / ER to Golgi transport vesicle membrane / regulation of erythrocyte differentiation / peptide antigen assembly with MHC class I protein complex / response to molecule of bacterial origin / regulation of iron ion transport / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / cellular response to iron ion / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / recycling endosome membrane / specific granule lumen / phagocytic vesicle membrane / peptide antigen binding / positive regulation of cellular senescence / antigen processing and presentation of exogenous peptide antigen via MHC class II / negative regulation of epithelial cell proliferation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / melanosome / sensory perception of smell / positive regulation of T cell activation / positive regulation of protein binding / tertiary granule lumen / DAP12 signaling / negative regulation of neuron projection development / MHC class II protein complex binding / late endosome membrane / T cell differentiation in thymus / ER-Phagosome pathway / iron ion transport / early endosome membrane / protein refolding / protein homotetramerization / intracellular iron ion homeostasis / amyloid fibril formation / learning or memory / immune response / Amyloid fiber formation / lysosomal membrane / external side of plasma membrane / endoplasmic reticulum lumen / Golgi membrane / signaling receptor binding / focal adhesion / Neutrophil degranulation / endoplasmic reticulum membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / Golgi apparatus / cell surface / endoplasmic reticulum / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / membrane / identical protein binding / plasma membrane / cytosol
Similarity search - Function
PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / PKAT, KLD domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / MHC class I, alpha chain, C-terminal ...PKD- and KLD-Associated Transmembrane / PKAT, KLD domain / PKAT, KLD domain / PKD domain / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
HLA class I histocompatibility antigen, A alpha chain / MHC class I antigen / Melanocyte protein PMEL / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.41 Å
AuthorsSmith, A.R. / Baker, B.M.
Funding support1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2021
Title: Structurally silent peptide anchor modifications allosterically modulate T cell recognition in a receptor-dependent manner
Authors: Smith, A.R. / Alonso, J.A. / Ayres, C.M. / Singh, N.K. / Hellman, L.M. / Baker, B.M.
History
DepositionJan 27, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 20, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 27, 2021Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
D: SILv44 T cell receptor alpha chain
E: SILv44 T cell receptor beta chain
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,9958
Polymers95,7185
Non-polymers2763
Water1,928107
1
A: MHC class I antigen, A-2 alpha chain
B: Beta-2-microglobulin
C: Melanocyte protein PMEL
hetero molecules

D: SILv44 T cell receptor alpha chain
E: SILv44 T cell receptor beta chain


Theoretical massNumber of molelcules
Total (without water)95,9958
Polymers95,7185
Non-polymers2763
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation4_545x+1/2,-y-1/2,-z1
Buried area11710 Å2
ΔGint-54 kcal/mol
Surface area37800 Å2
MethodPISA
Unit cell
Length a, b, c (Å)68.118, 94.403, 143.849
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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SILv44 T cell receptor ... , 2 types, 2 molecules DE

#1: Protein SILv44 T cell receptor alpha chain


Mass: 23287.006 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRAV8-1, TRAC / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS
#2: Protein SILv44 T cell receptor beta chain


Mass: 27692.809 Da / Num. of mol.: 1 / Mutation: 0
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRBV19, TRBC2 / Plasmid: pGMT7 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): pLysS

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Protein , 2 types, 2 molecules AB

#3: Protein MHC class I antigen, A-2 alpha chain


Mass: 31854.203 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: A0A5B8RNS7, UniProt: A0A140T913*PLUS
#4: Protein Beta-2-microglobulin / Beta-2 microglobulin


Mass: 11879.356 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Plasmid: pHN1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1-Blue / References: UniProt: P61769

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Protein/peptide , 1 types, 1 molecules C

#5: Protein/peptide Melanocyte protein PMEL / / ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma- ...ME20-M / ME20M / Melanocyte protein Pmel 17 / Melanocytes lineage-specific antigen GP100 / Melanoma-associated ME20 antigen / P1 / P100 / Premelanosome protein / Silver locus protein homolog


Mass: 1005.121 Da / Num. of mol.: 1 / Fragment: epitope (UNP residues 209-217) / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P40967

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Non-polymers , 2 types, 110 molecules

#6: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O3
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 107 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.42 Å3/Da / Density % sol: 49.09 %
Crystal growTemperature: 296 K / Method: vapor diffusion, hanging drop / Details: 16% v/v PEG3350, 280 mM ammonium citrate dibasic

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 24, 2018
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.41→50 Å / Num. obs: 35998 / % possible obs: 98.8 % / Redundancy: 6 % / Biso Wilson estimate: 39.57 Å2 / CC1/2: 0.99 / CC star: 0.998 / Rmerge(I) obs: 0.126 / Rpim(I) all: 0.055 / Rrim(I) all: 0.138 / Χ2: 0.933 / Net I/σ(I): 13.1
Reflection shellResolution: 2.41→2.45 Å / Redundancy: 5.1 % / Rmerge(I) obs: 0.566 / Mean I/σ(I) obs: 2 / Num. unique obs: 1725 / CC1/2: 0.779 / CC star: 0.936 / Rpim(I) all: 0.267 / Rrim(I) all: 0.629 / Χ2: 0.746 / % possible all: 97

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Processing

Software
NameVersionClassification
PHENIX1.13_2998refinement
PHENIX1.13_2998refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 1TVH

1tvh


Resolution: 2.41→39.46 Å / SU ML: 0.3262 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 24.9829
RfactorNum. reflection% reflection
Rfree0.2412 1921 5.58 %
Rwork0.1992 --
obs0.2015 34444 94.66 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 50.71 Å2
Refinement stepCycle: LAST / Resolution: 2.41→39.46 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6652 0 18 107 6777
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00236855
X-RAY DIFFRACTIONf_angle_d0.49459304
X-RAY DIFFRACTIONf_chiral_restr0.0428967
X-RAY DIFFRACTIONf_plane_restr0.00271213
X-RAY DIFFRACTIONf_dihedral_angle_d17.8632507
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.41-2.470.33281220.28832026X-RAY DIFFRACTION83.94
2.47-2.540.3591280.28272128X-RAY DIFFRACTION87.99
2.54-2.620.38741250.27782170X-RAY DIFFRACTION89.54
2.62-2.70.31311290.26552184X-RAY DIFFRACTION90.74
2.7-2.80.31061280.25372189X-RAY DIFFRACTION90.93
2.8-2.910.28311390.24232326X-RAY DIFFRACTION95.47
2.91-3.040.26851400.23352367X-RAY DIFFRACTION96.95
3.04-3.20.24421390.22052367X-RAY DIFFRACTION97.59
3.2-3.40.27761420.21142427X-RAY DIFFRACTION98.85
3.4-3.670.26421440.19822418X-RAY DIFFRACTION99.26
3.67-4.030.21431410.172383X-RAY DIFFRACTION96.37
4.03-4.620.17061450.15142457X-RAY DIFFRACTION99.31
4.62-5.810.19321480.16382509X-RAY DIFFRACTION99.89
5.81-39.460.22361510.18572572X-RAY DIFFRACTION97.74
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.087701623332.89971075221-2.14266503953.2559532519-0.8024391969632.170905563560.374662169187-0.743690781170.8209593254330.0985902694245-0.101132030305-0.345650898855-0.1347388307430.824669994593-0.2198818661010.314238491523-0.0508994766458-0.05116839508580.462111360275-0.1337634381720.51013847852-21.9384431826-19.857699975429.2722224201
27.798131058950.932739460336-2.283004659624.07287496244-0.2429471649942.96196547263-0.2871146654640.2027290256590.0760809385273-0.1826442639420.236748710647-0.2392714396150.0433416766231-0.03639886575490.05283798137820.219034766909-0.0333458882203-0.02062432588770.301118475937-0.05130767344840.230037644932-30.0181840932-23.830466099521.9185273695
37.871353567826.81708637383-1.084995545986.8844651221-2.461269105842.38937564392-0.058661888334-0.804813047669-0.175695840392-0.42092550919-0.206433954749-0.5843678560630.07253568706120.4221865224170.1983799589830.320024804543-0.0635344213844-0.02519086240880.48408698617-0.1727854108490.889547477542-8.18261950875-9.5935920659322.8665398578
44.5950150089-0.4780297922570.6685254406190.0704144623313-0.3772402375786.5495651759-0.03224458747550.0905964102857-0.125418308796-0.01496456601230.114620175567-0.419131645526-0.3943998894560.251690635351-0.04130302565110.463510907882-0.1507232951960.1317134250820.402582050173-0.1612861075170.560316972484-7.122826167979.6555569061523.1864932797
57.194233140350.015864488356-0.2300245896445.289654194651.543561220939.584942327660.03430766173880.232684037926-1.11362606611-0.000399940594642-0.076232935351-0.01574677979751.27507116897-0.06015867526340.1753028352350.367112056114-0.03044320995490.02560975263230.406026072745-0.1067724558090.549730136169-10.7515671065-0.38408538866618.7072547259
65.44228948471-2.05639894495-0.5007321997168.423458333430.1019322101083.02820395687-0.0524420177832-0.1725094251650.413642385390.02087082898850.365375260031-1.11515257946-0.2709810974920.746584354927-0.3209076499910.418195418594-0.0834071733570.02284744896280.675597664064-0.1293162567450.600901662086-3.988664275739.5339136191125.3008497053
73.063406429911.160802199410.8509147904685.977124603872.368848972983.95640822919-0.139464866817-0.2866415899470.4011293842350.042026671246-0.04934987967840.0594146585845-0.449079894954-0.0218163035980.1481182460180.285713602023-0.0100352496938-0.02255604752060.321901395062-0.06164982166250.412252531875-45.0367906984-7.4915847347521.6629637888
84.260643850282.309250533160.4681903674022.29697848928-0.1915230969192.306237777920.2912862985260.1155327455160.08361114934080.469979683274-0.9012259273880.1090612315240.00573385471428-0.6528570640560.5857101877580.609511973904-0.0708671032623-0.1352353006630.326347465987-0.07376237020760.659559812824-35.441473679112.915119632926.0771742081
92.19812100429-1.36724059833-1.095240745711.933935214970.9886490350862.3082980366-0.04632142572230.00692624337622-0.177778816487-0.2914971705540.048772090125-0.0456979771992-0.03445698974450.0983061798617-0.07755065682220.493961176221-0.1002528370630.05900544369410.37908831181-0.1305237552280.439743749128-16.02056277518.0600382241314.4564807335
108.00400065284-2.79293950647-6.007044546443.967763440622.022870326347.72448990549-0.1300340762080.6413909684070.502061784175-0.4608410816610.06442336243310.36525609727-0.41455139803-0.8226535273070.01292644583250.596137695377-0.0369411388049-0.1577552668220.435254480723-0.002522412714020.482036975681-30.982264350717.47065377714.0113977191
111.61872062750.121062381223-0.9239904630392.43435272906-0.3414655379082.810959117870.008218001160190.06854209576530.03673219915440.0211800553815-0.00165326593267-0.0235204156423-0.075653908312-0.0103493536361-0.01133113058570.2051296241740.0199724947052-0.01498756960730.294577211691-0.05814512284970.384970536183-22.955409819-13.8957753795-13.105160795
124.69683137969-3.04252068528-1.977116737075.204603223531.951139496683.28743218816-0.205945563512-0.5318338283280.3227426932460.5779338493010.1753542389180.135946445434-0.135502207494-0.120267934766-0.024268339050.5036153106820.0867298348041-0.08279808834760.403728348549-0.1015562719810.448968227187-40.203410136915.6866060487-6.48973936095
134.2185259489-0.5669764099311.794276099829.96032099832-8.174772144779.77251756290.130657774326-0.5723223741630.04456729401760.6475224469590.1308363983720.729427056755-0.3613606243030.163226343716-0.3637530039220.3152962735250.07772272410720.06818821366950.494278388076-0.04884947094330.360800381719-44.2365946426-6.09169194883-7.10110935133
149.38973424256-2.107722048366.519043612210.472176199127-1.458634586814.64618264707-0.09024618056180.9186269237861.28101230638-0.733046443771-0.874028973638-0.0911135267025-1.771152743910.2538934895690.9418239153980.5424966449410.0566938333265-0.1899595889590.5273088341680.06282668957710.715394211322-48.217078175911.775790419-24.4287237592
153.887155245780.131760547768-0.7899583961882.172676500380.1429116131591.78918693938-0.3569534382560.1019849483330.182503240903-0.167465888843-0.0563166252163-0.207202883811-0.35080496065-0.5873195008320.3069240864820.288725372040.0712550013727-0.0826242142610.35629655596-0.1059817975960.414685988898-44.4579626265-0.529869308476-16.9792819858
167.15578118362-2.899718832713.493162689564.14327928886-2.110219027033.98560392294-0.01272728324230.124729129497-0.287185622814-0.501924411990.02101960890050.492267379357-0.0381421734709-0.255935718155-0.05839118810330.3091259530110.0171569675423-0.0579390754680.323719681434-0.130257253710.490629634845-44.3388841221-6.89796539695-17.6352037109
178.00829117933-4.506906943652.756534577143.59026982126-0.8200551975922.21530744430.1045644275660.7336629088051.450954946010.03391245777180.2731772967951.60739286592-0.947401489388-1.30857429008-0.4247462716670.680899248380.256667560831-0.2566581184150.674181539297-0.0235037486390.935106141942-60.09607434647.70110731696-23.5452435275
185.69161727181-6.516764716125.33642447027.9795930993-5.339523308956.33669855616-0.0140561038159-0.259432715004-0.0213685536843-0.0406175985920.3816879120451.373818278890.0966664246809-0.624254195151-0.4499123436890.297999151365-0.013553878804-0.1045528064610.460424885353-0.008042717232170.728783478168-52.7874959649-9.57699449895-10.7677444894
198.666067903991.27873734749-1.060397277746.11085476957-2.748125640788.26505984469-0.25900271885-0.444699055571.103290772810.2986436789470.08465510831640.564494710547-0.519729072889-0.1955783700440.05538850385740.3594729742920.144021573882-0.06571825231690.444289171538-0.1004883003190.629516383413-54.5124765644.92569880445-13.1929973997
202.61108728825-2.746359751881.616365692173.72636579641-1.007645454071.719833495110.0510516081835-0.205213162590.2047535120370.136614015339-0.009968751305010.209403562969-0.2981414946640.246827911901-0.005264351914580.230843240494-0.05027218470830.02187298228680.268944348124-0.08109436958270.42437137614-17.7713062269-19.4070136471-14.0135144238
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'D' and (resid 3 through 22 )
2X-RAY DIFFRACTION2chain 'D' and (resid 23 through 107 )
3X-RAY DIFFRACTION3chain 'D' and (resid 108 through 121 )
4X-RAY DIFFRACTION4chain 'D' and (resid 122 through 155 )
5X-RAY DIFFRACTION5chain 'D' and (resid 156 through 170 )
6X-RAY DIFFRACTION6chain 'D' and (resid 171 through 203 )
7X-RAY DIFFRACTION7chain 'E' and (resid 2 through 107 )
8X-RAY DIFFRACTION8chain 'E' and (resid 108 through 122 )
9X-RAY DIFFRACTION9chain 'E' and (resid 123 through 213 )
10X-RAY DIFFRACTION10chain 'E' and (resid 214 through 242 )
11X-RAY DIFFRACTION11chain 'A' and (resid 1 through 174 )
12X-RAY DIFFRACTION12chain 'A' and (resid 175 through 275 )
13X-RAY DIFFRACTION13chain 'B' and (resid 1 through 12 )
14X-RAY DIFFRACTION14chain 'B' and (resid 13 through 20 )
15X-RAY DIFFRACTION15chain 'B' and (resid 21 through 31 )
16X-RAY DIFFRACTION16chain 'B' and (resid 32 through 72 )
17X-RAY DIFFRACTION17chain 'B' and (resid 73 through 78 )
18X-RAY DIFFRACTION18chain 'B' and (resid 79 through 91 )
19X-RAY DIFFRACTION19chain 'B' and (resid 92 through 100 )
20X-RAY DIFFRACTION20chain 'C' and (resid 1 through 9 )

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