- PDB-6uhm: Crystal Structure of a Physical Mixture of C148 mGFP and scDNA-1 -
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基本情報
登録情報
データベース: PDB / ID: 6uhm
タイトル
Crystal Structure of a Physical Mixture of C148 mGFP and scDNA-1
要素
C148 mGFP
キーワード
FLUORESCENT PROTEIN (蛍光タンパク質) / green fluorescent protein (緑色蛍光タンパク質) / beta-barrel (Βバレル) / protein-DNA mixture
機能・相同性
Green fluorescent protein, GFP / Green fluorescent protein-related / 緑色蛍光タンパク質 / 緑色蛍光タンパク質 / 生物発光 / generation of precursor metabolites and energy / 緑色蛍光タンパク質
分子量: 31038.908 Da / 分子数: 2 / 変異: S148C / 由来タイプ: 組換発現 詳細: This mutant of EGFP has a single surface cysteine at residue 148 (counting from M37 and counting CRO as 3 residues) and an N-terminal his-tag. 由来: (組換発現) Aequorea victoria (オワンクラゲ) 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 参照: UniProt: P42212*PLUS
マシュー密度: 1.96 Å3/Da / 溶媒含有率: 37.29 % / 解説: Green crystal with sharp facets
結晶化
温度: 295 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.4 詳細: 1 microliter of a physical mixture of C148 mGFP and scDNA-1 (5 mg/mL C148 mGFP and equimolar scDNA-1 in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M ...詳細: 1 microliter of a physical mixture of C148 mGFP and scDNA-1 (5 mg/mL C148 mGFP and equimolar scDNA-1 in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol) in a sitting drop with a 70 microliter reservoir (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol)
解像度: 2.1→56.25 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.928 / WRfactor Rfree: 0.2788 / WRfactor Rwork: 0.2201 / FOM work R set: 0.7653 / SU B: 7.102 / SU ML: 0.181 / SU R Cruickshank DPI: 0.2426 / SU Rfree: 0.2117 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.243 / ESU R Free: 0.212 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2701
1512
5.2 %
RANDOM
Rwork
0.2107
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obs
0.2139
27645
99.61 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK