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Yorodumi- PDB-6tl0: Solution structure and 1H, 13C and 15N chemical shift assignments... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6tl0 | |||||||||||||||
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Title | Solution structure and 1H, 13C and 15N chemical shift assignments for the complex of VPS29 with VARP 687-747 | |||||||||||||||
Components |
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Keywords | INTRACELLULAR VESICLE TRAFFICKING / VARP / retromer / NMR complex structure / Zinc finger / endosome | |||||||||||||||
Function / homology | Function and homology information WNT ligand biogenesis and trafficking / negative regulation of SNARE complex assembly / tubular endosome / endosome to melanosome transport / retromer, cargo-selective complex / Golgi to vacuole transport / retromer complex / early endosome to late endosome transport / RAB GEFs exchange GTP for GDP on RABs / endocytic recycling ...WNT ligand biogenesis and trafficking / negative regulation of SNARE complex assembly / tubular endosome / endosome to melanosome transport / retromer, cargo-selective complex / Golgi to vacuole transport / retromer complex / early endosome to late endosome transport / RAB GEFs exchange GTP for GDP on RABs / endocytic recycling / retrograde transport, endosome to Golgi / positive regulation of dendrite morphogenesis / transport vesicle / GTPase activator activity / SNARE binding / guanyl-nucleotide exchange factor activity / neuron projection morphogenesis / intracellular protein transport / cytoplasmic vesicle membrane / small GTPase binding / positive regulation of neuron projection development / melanosome / late endosome / protein transport / lysosome / early endosome / endosome membrane / endosome / neuron projection / intracellular membrane-bounded organelle / membrane / metal ion binding / plasma membrane / cytosol Similarity search - Function | |||||||||||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | |||||||||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||||||||
Authors | Owen, D.J. / Neuhaus, D. / Yang, J.-C. / Crawley-Snowdon, H. | |||||||||||||||
Funding support | United Kingdom, Canada, 4items
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Citation | Journal: Nat Commun / Year: 2020 Title: Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29. Authors: Crawley-Snowdon, H. / Yang, J.C. / Zaccai, N.R. / Davis, L.J. / Wartosch, L. / Herman, E.K. / Bright, N.A. / Swarbrick, J.S. / Collins, B.M. / Jackson, L.P. / Seaman, M.N.J. / Luzio, J.P. / ...Authors: Crawley-Snowdon, H. / Yang, J.C. / Zaccai, N.R. / Davis, L.J. / Wartosch, L. / Herman, E.K. / Bright, N.A. / Swarbrick, J.S. / Collins, B.M. / Jackson, L.P. / Seaman, M.N.J. / Luzio, J.P. / Dacks, J.B. / Neuhaus, D. / Owen, D.J. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6tl0.cif.gz | 1.8 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6tl0.ent.gz | 1.5 MB | Display | PDB format |
PDBx/mmJSON format | 6tl0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tl/6tl0 ftp://data.pdbj.org/pub/pdb/validation_reports/tl/6tl0 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 21626.832 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Vps29 / Plasmid: pGEXVPS29 / Details (production host): cDNA Cloned EcoRI into pGEX4T1 / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): DE3 pLysS / References: UniProt: Q9QZ88 |
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#2: Protein | Mass: 6319.927 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ANKRD27, PP12899 / Plasmid: pGEXVARPfingernail2 / Details (production host): cDNA clone BamHI/NotI pGEX6P1 / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): DE3 pLysS / References: UniProt: Q96NW4 |
#3: Chemical | ChemComp-ZN / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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