modulation of formation of structure involved in a symbiotic process / rRNA (cytosine-N4-)-methyltransferase activity / ATP-activated inward rectifier potassium channel activity / kinetoplast / quorum sensing / rRNA base methylation / response to arsenic-containing substance / ciliary plasm / mitochondrial small ribosomal subunit / voltage-gated potassium channel activity ...modulation of formation of structure involved in a symbiotic process / rRNA (cytosine-N4-)-methyltransferase activity / ATP-activated inward rectifier potassium channel activity / kinetoplast / quorum sensing / rRNA base methylation / response to arsenic-containing substance / ciliary plasm / mitochondrial small ribosomal subunit / voltage-gated potassium channel activity / mitochondrial respiratory chain complex I / acyl binding / acyl carrier activity / voltage-gated potassium channel complex / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / protein homooligomerization / potassium ion transport / fatty acid biosynthetic process / methylation / mitochondrial matrix / structural constituent of ribosome / translation / mRNA binding / mitochondrion / membrane / cytosol / cytoplasm Similarity search - Function
T-cell activation inhibitor, mitochondrial / Domain of unknown function DUF4460 / Domain of unknown function (DUF4460) / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Ribosomal protein Rsm22-like / Mitochondrial small ribosomal subunit Rsm22 ...T-cell activation inhibitor, mitochondrial / Domain of unknown function DUF4460 / Domain of unknown function (DUF4460) / Probable Zinc-ribbon domain / Probable Zinc-ribbon domain / Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Ribosomal protein Rsm22-like / Mitochondrial small ribosomal subunit Rsm22 / Protein Fyv4 / IGR protein motif / IGR / Phosphotyrosine protein phosphatase I / Phosphotyrosine protein phosphatase I superfamily / Low molecular weight phosphotyrosine protein phosphatase / Low molecular weight phosphatase family / Pentatricopeptide repeat domain / Pentatricopeptide (PPR) repeat profile. / Pentatricopeptide repeat / Potassium channel tetramerisation-type BTB domain / BTB/POZ domain / Acyl carrier protein (ACP) / SKP1/BTB/POZ domain superfamily / Phosphopantetheine attachment site / Phosphopantetheine attachment site. / Phosphopantetheine attachment site / ACP-like superfamily / Carrier protein (CP) domain profile. / Phosphopantetheine binding ACP domain / Translation elongation factor EF1B/ribosomal protein S6 / Tetratricopeptide-like helical domain superfamily / Ribosomal protein S11 superfamily / S15/NS1, RNA-binding / Thioredoxin-like superfamily / S-adenosyl-L-methionine-dependent methyltransferase superfamily Similarity search - Domain/homology
Chem-PM8 / PHOSPHATE ION / RNA / RNA (> 10) / RNA (> 100) / Uncharacterized protein / Uncharacterized protein / Methyltransferase / Uncharacterized protein / Potassium voltage-gated channel, putative ...Chem-PM8 / PHOSPHATE ION / RNA / RNA (> 10) / RNA (> 100) / Uncharacterized protein / Uncharacterized protein / Methyltransferase / Uncharacterized protein / Potassium voltage-gated channel, putative / Uncharacterized protein / Small ribosomal subunit protein mS41 / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Target SNARE / Phosphotyrosine protein phosphatase I domain-containing protein / DUF4460 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Uncharacterized protein / Glutaredoxin domain-containing protein / S-adenosyl-methyltransferase mraW-like protein, putative / Acyl carrier protein / Pentacotripeptide-repeat region of PRORP domain-containing protein / Probable Zinc-ribbon domain-containing protein / Succinate dehydrogenase assembly factor 3, mitochondrial / Uncharacterized protein Similarity search - Component
Biological species
Trypanosoma brucei brucei (eukaryote)
Method
ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å
Journal: Science / Year: 2019 Title: Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery. Authors: Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut ...Authors: Martin Saurer / David J F Ramrath / Moritz Niemann / Salvatore Calderaro / Céline Prange / Simone Mattei / Alain Scaiola / Alexander Leitner / Philipp Bieri / Elke K Horn / Marc Leibundgut / Daniel Boehringer / André Schneider / Nenad Ban / Abstract: Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome ...Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.
Protein , 48 types, 54 molecules CECFCHCKCOCPCQCRCaCbCdCjCnCpDDDIDLDODPDRDUDZF2F3F5F6F7F8F9FA...
#1: Protein
uS5m
Mass: 50147.355 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#2: Protein
bS6m
Mass: 18861.543 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38BW5
#3: Protein
uS8m
Mass: 32766.459 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#4: Protein
uS11m
Mass: 37953.965 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q389T7
#5: Protein
uS15m
Mass: 50401.191 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q4GZ99
#6: Protein
bS16m
Mass: 22077.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q384N9
#7: Protein
uS17m
Mass: 39012.414 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence; C-terminal PTP-tag Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#8: Protein
bS18m
Mass: 36988.691 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#9: Protein
mS22
Mass: 73064.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38DR3
#10: Protein
mS23
Mass: 35358.805 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#11: Protein
mS26
Mass: 51136.684 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38DK6
#12: Protein
mS34
Mass: 28728.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57UK0
#13: Protein
mS38
Mass: 28240.600 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57VQ9
#14: Protein
mS41
Mass: 22421.469 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q389L3
#15: Protein
mS51 (KRIPP1)
Mass: 95080.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#16: Protein
mS56
Mass: 47306.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#17: Protein
mS59
Mass: 35395.676 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#18: Protein
mS62 (KRIPP14)
Mass: 32068.100 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q383D1
#19: Protein
mS63 (KRIPP16)
Mass: 31922.178 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#20: Protein
mS65
Mass: 30617.381 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#21: Protein
mS68
Mass: 26472.807 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#22: Protein
mS73
Mass: 11360.885 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q587C4
#23: Protein
mt-SAF2 (KRIPP2)
Mass: 116189.547 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#24: Protein
mt-SAF3
Mass: 106621.727 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38E61
#25: Protein
mt-SAF5
Mass: 86949.414 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#26: Protein
mt-SAF6
Mass: 76781.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38FQ8
#27: Protein
mt-SAF7 (KRIPP10)
Mass: 76675.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57UW6
#28: Protein
mt-SAF8
Mass: 76725.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#29: Protein
mt-SAF9
Mass: 69781.789 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#30: Protein
mt-SAF10
Mass: 69306.758 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q386U1
#31: Protein
mt-SAF11
Mass: 64916.957 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#32: Protein
mt-SAF13
Mass: 63690.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#33: Protein
mt-SAF18
Mass: 42508.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#34: Protein
mt-SAF21
Mass: 39610.793 Da / Num. of mol.: 2 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#35: Protein
mt-SAF22 (KRIPP17)
Mass: 39132.621 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q389F9
#36: Protein
mt-SAF23
Mass: 38240.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#37: Protein
mt-SAF24
Mass: 34648.047 Da / Num. of mol.: 5 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q387P8
#38: Protein
mt-SAF26
Mass: 30538.557 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q38FI3
#39: Protein
mt-SAF27
Mass: 26936.748 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57YK5
#40: Protein
mt-SAF28
Mass: 21468.707 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#41: Protein
mt-SAF29
Mass: 20048.514 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#42: Protein
mt-SAF30
Mass: 19659.756 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57VU7
#43: Protein
mt-SAF31
Mass: 17673.535 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q581U4
#44: Protein
mt-SAF32
Mass: 16550.807 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57WW9
#45: Protein
mt-SAF33
Mass: 16291.121 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q581T4
#59: Protein
UNK-I
Mass: 39847.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427
#61: Protein
mt-SAF1 (RSM22)
Mass: 119799.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q385R2
#62: Protein
mt-SAF14
Mass: 53320.820 Da / Num. of mol.: 1 / Source method: isolated from a natural source Details: Protein of Trypanosoma brucei brucei Lister strain 427; sequence accessible on TriTrypDB; where necessary, the reference strain TREU927 was used to complete the sequence Source: (natural) Trypanosoma brucei brucei (eukaryote) / Strain: Lister strain 427 / References: UniProt: Q57W60
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES Details: Body domain of the mitochondrial ribosomal small subunit assemblosome of Trypanosoma brucei brucei Lister strain 427
Electron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)
-
Processing
EM software
ID
Name
Category
2
EPU
imageacquisition
4
Gctf
CTFcorrection
9
RELION
initialEulerassignment
10
RELION
finalEulerassignment
12
RELION
3Dreconstruction
CTF correction
Details: On the fly in RELION / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Symmetry
Point symmetry: C1 (asymmetric)
3D reconstruction
Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 161661 / Algorithm: FOURIER SPACE / Symmetry type: POINT
Atomic model building
Protocol: OTHER Details: Starting models of ribosomal proteins of the T. brucei mitochondrial ribosomal small subunit were taken from PDB 6HIW. Mitchondrial ribosomal small subunit assembly factors were built de novo.
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