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Yorodumi- PDB-6scq: Cell Division Protein SepF in complex with C-terminal domain of FtsZ -
+Open data
-Basic information
Entry | Database: PDB / ID: 6scq | ||||||
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Title | Cell Division Protein SepF in complex with C-terminal domain of FtsZ | ||||||
Components | Cell division protein SepF | ||||||
Keywords | CELL CYCLE / Cell Division protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Corynebacterium glutamicum ATCC 13032 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Sogues, A. / Wehenkel, A.M. / Alzari, P.M. | ||||||
Funding support | France, 1items
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Citation | Journal: Nat Commun / Year: 2020 Title: Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum. Authors: Sogues, A. / Martinez, M. / Gaday, Q. / Ben Assaya, M. / Grana, M. / Voegele, A. / VanNieuwenhze, M. / England, P. / Haouz, A. / Chenal, A. / Trepout, S. / Duran, R. / Wehenkel, A.M. / Alzari, P.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6scq.cif.gz | 68.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6scq.ent.gz | 49.8 KB | Display | PDB format |
PDBx/mmJSON format | 6scq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sc/6scq ftp://data.pdbj.org/pub/pdb/validation_reports/sc/6scq | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 8147.301 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Corynebacterium glutamicum ATCC 13032 (bacteria) Gene: sepF, Cgl2152 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q8NNN6 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.44 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6 / Details: 0.1 M MES pH 6, 20 %w/v PEG MME 2K, 0.2 M NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.97625 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 13, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→32.91 Å / Num. obs: 21921 / % possible obs: 95.2 % / Redundancy: 2.6 % / CC1/2: 0.999 / Rmerge(I) obs: 0.026 / Net I/σ(I): 19.6 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.166 / Mean I/σ(I) obs: 4.9 / Num. unique obs: 1127 / CC1/2: 0.985 / % possible all: 97.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.5→29.92 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.934 / SU R Cruickshank DPI: 0.082 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.084 / SU Rfree Blow DPI: 0.076 / SU Rfree Cruickshank DPI: 0.076
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Displacement parameters | Biso max: 138.16 Å2 / Biso mean: 39.64 Å2 / Biso min: 9.58 Å2
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Refine analyze | Luzzati coordinate error obs: 0.21 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.5→29.92 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.51 Å / Rfactor Rfree error: 0 / Total num. of bins used: 50
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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