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- PDB-6s32: Crystal structure of ene-reductase CtOYE from Chroococcidiopsis t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6s32 | |||||||||
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Title | Crystal structure of ene-reductase CtOYE from Chroococcidiopsis thermalis. | |||||||||
![]() | NADH:flavin oxidoreductase/NADH oxidase | |||||||||
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Function / homology | ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Robescu, M.R. / Niero, M. / Hall, M. / Bergantino, E. / Cendron, L. | |||||||||
![]() | ![]() Title: Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE. Authors: Robescu, M.S. / Niero, M. / Hall, M. / Cendron, L. / Bergantino, E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 608.4 KB | Display | ![]() |
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PDB format | ![]() | 497.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6s0gC ![]() 6s23C ![]() 6s31C ![]() 1gwjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 42628.355 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-ACT / ![]() #3: Chemical | ChemComp-BEN / | ![]() #4: Chemical | ChemComp-FMN / ![]() #5: Water | ChemComp-HOH / | ![]() Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 52.99 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.85 M sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.0, 0.1 M NaCl, 0.10 mM Fos-Choline 12 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Jul 10, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.35→48.86 Å / Num. obs: 312849 / % possible obs: 90.89 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.086 / Net I/σ(I): 5.76 |
Reflection shell | Resolution: 1.35→1.39 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.535 / Mean I/σ(I) obs: 1.63 / Num. unique obs: 29361 / % possible all: 85.78 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1GWJ Resolution: 1.35→48.86 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.916 / SU B: 3.004 / SU ML: 0.055 / Cross valid method: THROUGHOUT / ESU R: 0.076 / ESU R Free: 0.074 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.791 Å2
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Refinement step | Cycle: LAST / Resolution: 1.35→48.86 Å
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Refine LS restraints |
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