+Open data
-Basic information
Entry | Database: PDB / ID: 6rvq | ||||||
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Title | SaFtsz-GDP-EthGLy | ||||||
Components | Cell division protein FtsZ | ||||||
Keywords | CELL CYCLE / Cell division protein | ||||||
Function / homology | Function and homology information FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.136 Å | ||||||
Authors | Fernandez-Tornero, C. / Andreu, J.M. | ||||||
Citation | Journal: Febs J. / Year: 2020 Title: Nucleotide-induced folding of cell division protein FtsZ from Staphylococcus aureus. Authors: Huecas, S. / Canosa-Valls, A.J. / Araujo-Bazan, L. / Ruiz, F.M. / Laurents, D.V. / Fernandez-Tornero, C. / Andreu, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6rvq.cif.gz | 126.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6rvq.ent.gz | 97.2 KB | Display | PDB format |
PDBx/mmJSON format | 6rvq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rv/6rvq ftp://data.pdbj.org/pub/pdb/validation_reports/rv/6rvq | HTTPS FTP |
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-Related structure data
Related structure data | 6rvmC 6rvnSC 6rvpC 6si9C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31825.014 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: ftsZ / Production host: Escherichia coli (E. coli) / References: UniProt: P0A031 |
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#2: Chemical | ChemComp-GDP / |
#3: Chemical | ChemComp-CA / |
#4: Chemical | ChemComp-EDO / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.92 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 27.5 % PEG 5K MME, 10 % EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 9.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.9793 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 1, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.136→40 Å / Num. obs: 108093 / % possible obs: 99.1 % / Redundancy: 3.3 % / CC1/2: 0.998 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 1.14→1.2 Å / Num. unique obs: 15595 / CC1/2: 0.716 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6RVN Resolution: 1.136→33.464 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 0.32 / Phase error: 29.19
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.136→33.464 Å
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Refine LS restraints |
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LS refinement shell |
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