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- PDB-6rjw: Crystal structure of the N-terminal domain of Lyme disease agent ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rjw | ||||||
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Title | Crystal structure of the N-terminal domain of Lyme disease agent Borrelia burgdorferi major virulence factor BB0323 (Se-Met data) | ||||||
![]() | LysM domain protein![]() | ||||||
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Function / homology | LysM domain superfamily / ![]() ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brangulis, K. / Akopjana, I. / Kazaks, A. / Tars, K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferi. Authors: Brangulis, K. / Akopjana, I. / Kazaks, A. / Tars, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.9 KB | Display | ![]() |
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PDB format | ![]() | 32.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | ![]() Mass: 22436.779 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: The first 4 residues (GAMG) are remnants from the expression tag. Source: (gene. exp.) ![]() Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680 / Gene: BB_0323 / Production host: ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.32 % |
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Crystal grow![]() | Temperature: 294 K / Method: vapor diffusion, sitting drop / Details: 0.2 M KNO3 18% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 9, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.85→43.9 Å / Num. obs: 4510 / % possible obs: 100 % / Redundancy: 12.6 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.85→3 Å / Redundancy: 13.4 % / Rmerge(I) obs: 0.219 / Mean I/σ(I) obs: 15.6 / Num. unique obs: 636 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 104.11 Å2 / Biso mean: 39.171 Å2 / Biso min: 16.72 Å2
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Refinement step | Cycle: final / Resolution: 2.85→43.9 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.85→2.924 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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