- PDB-6qsm: mTFP* open conformation: I197C-Y200H-Y204H mutant for enhanced me... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6qsm
Title
mTFP* open conformation: I197C-Y200H-Y204H mutant for enhanced metal binding
Components
GFP-like fluorescent chromoprotein cFP484
Keywords
FLUORESCENT PROTEIN / BETA BARREL / ARTIFICIAL METALLOENZYME / BIOCONJUGATION / PROTEIN DESIGN / THERMOSTABLE
Function / homology
Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / GFP-like fluorescent chromoprotein cFP484
Function and homology information
Biological species
Clavularia sp. (invertebrata)
Method
X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.65 Å
Resolution: 1.65→15 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.946 / SU B: 4.656 / SU ML: 0.069 / Cross valid method: THROUGHOUT / ESU R: 0.155 / ESU R Free: 0.097 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.19612
1209
5 %
RANDOM
Rwork
0.15211
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obs
0.15274
23005
99.68 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å