+Open data
-Basic information
Entry | Database: PDB / ID: 6ooq | ||||||
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Title | protein C | ||||||
Components | Multidrug transporter MdfA | ||||||
Keywords | TRANSPORT PROTEIN / Complex | ||||||
Function / homology | Function and homology information potassium:proton antiporter activity / sodium:proton antiporter activity / xenobiotic detoxification by transmembrane export across the plasma membrane / regulation of cellular pH / transmembrane transporter activity / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 3 Å | ||||||
Authors | Lu, M. | ||||||
Funding support | United States, 1items
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Citation | Journal: Commun Biol / Year: 2019 Title: Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition. Authors: Wu, H.H. / Symersky, J. / Lu, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ooq.cif.gz | 88.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ooq.ent.gz | 65.4 KB | Display | PDB format |
PDBx/mmJSON format | 6ooq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oo/6ooq ftp://data.pdbj.org/pub/pdb/validation_reports/oo/6ooq | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 42492.570 Da / Num. of mol.: 1 / Fragment: residues 9-400 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: A9R57_05385, BJJ90_17655 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1E5MBX7, UniProt: P0AEY8*PLUS |
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#2: Chemical | ChemComp-DXC / ( |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.18 Å3/Da / Density % sol: 70.58 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å |
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: Oct 10, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3→15 Å / Num. obs: 10817 / % possible obs: 98 % / Redundancy: 15 % / Net I/σ(I): 40 |
Reflection shell | Resolution: 3→3.1 Å / Num. unique obs: 300 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 3→15 Å / Cross valid method: FREE R-VALUE /
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Refinement step | Cycle: LAST / Resolution: 3→15 Å
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