PDB-6nvy: Crystal structure of penicillin G acylase from Bacillus thermotolerans

ID or keywords:

Entry

Database: PDB / ID: 6nvy

Title

Crystal structure of penicillin G acylase from Bacillus thermotolerans

Components

(Penicillin G acylase) x 2

Keywords

HYDROLASE /

penicillin / acylase

Function / homology

Function and homology information

hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides / antibiotic biosynthetic process /

metal ion binding
Similarity search - Function

Biological species

Quasibacillus thermotolerans (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 2.27 Å

Authors

Blankenfeldt, W.

Funding support

Germany, 1items

Citation

Journal: Appl.Microbiol.Biotechnol. / Year: 2019
Title: Crystal structures and protein engineering of three different penicillin G acylases from Gram-positive bacteria with different thermostability.
Authors: Mayer, J. / Pippel, J. / Gunther, G. / Muller, C. / Lauermann, A. / Knuuti, T. / Blankenfeldt, W. / Jahn, D. / Biedendieck, R.

History

Deposition	Feb 5, 2019	Deposition site: RCSB / Processing site: PDBE
Revision 1.0	Jul 3, 2019	Provider: repository / Type: Initial release
Revision 1.1	Sep 11, 2019	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2	Jan 24, 2024	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_symmetry

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	6nvy.cif.gz	861.1 KB	Display	PDBx/mmCIF format
PDB format	pdb6nvy.ent.gz	730.8 KB	Display	PDB format
PDBx/mmJSON format	6nvy.json.gz		Tree view	PDBx/mmJSON format
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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/nv/6nvy ftp://data.pdbj.org/pub/pdb/validation_reports/nv/6nvy	HTTPS FTP

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Related structure data

Related structure data	6nvwC 6nvxSC S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6nvw-d 4pel-2 4pel-3 5fir-5 5fir-1 1pnl-d 1k5s-d 4pel-1 1k5q-d 1h2g-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#166 - Oct 2013 Proteasome similarity (1) #80 - Aug 2006 AAA+ Proteases similarity (1) #176 - Aug 2014 Dynein similarity (1) #60 - Dec 2004 Ubiquitin similarity (1)

Deposited unit

A: Penicillin G acylase

B: Penicillin G acylase

C: Penicillin G acylase

D: Penicillin G acylase

hetero molecules

173 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Penicillin G acylase

B: Penicillin G acylase

hetero molecules

defined by software
Evidence: gel filtration
86.8 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: Penicillin G acylase

D: Penicillin G acylase

hetero molecules

defined by software
Evidence: gel filtration
86.7 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	172.996, 172.996, 209.566
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	146
Space group name H-M	H3

#1: Protein	Penicillin G acylase Mass: 24785.479 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Quasibacillus thermotolerans (bacteria) Gene: QY95_01219 / Production host: Bacillus megaterium (bacteria) / References: UniProt: A0A0F5I5V4
#2: Protein	Penicillin G acylase Mass: 61664.922 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Quasibacillus thermotolerans (bacteria) Gene: QY95_01219 / Production host: Bacillus megaterium (bacteria) / References: UniProt: A0A0F5I5V4
#3: Chemical	ChemComp-CA / CALCIUM ION Mass: 40.078 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: Ca
#4: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₃H₈O₃
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 721 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.49 Å³/Da / Density % sol: 64.76 %
Crystal grow	Temperature: 309 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 18 mM CaCl2, 90 mM sodium acetate, 27% (v/v) 2-methyl-2,4-pentanediol, 10% (v/v) glycerol

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

PETRA III, DESY

/ Beamline: P11 / Wavelength: 0.978 Å

Detector

Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 9, 2017

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 0.978 Å / Relative weight: 1

Reflection

Resolution: 2.27→49.45 Å / Num. obs: 108061 / % possible obs: 100 % / Redundancy: 10.5 % / CC_1/2: 0.999 / R_merge(I) obs: 0.114 / R_pim(I) all: 0.037 / R_rim(I) all: 0.12 / Net I/σ(I): 13.2

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Num. unique obs	CC_1/2	R_pim(I) all	R_rim(I) all	% possible all
2.27-2.31	10.5	1.549	5364	0.724	0.501	1.628	100
12.43-49.45	10.7	0.037	632	0.999	0.012	0.039	96.8

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Phasing

Phasing	Method: molecular replacement

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Processing

Software

Name	Version	Classification
XDS		data reduction
Aimless	0.7.3	data scaling
PHASER		phasing
PHENIX	dev_3386	refinement
PDB_EXTRACT	3.24	data extraction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 6NVX

6nvx

Resolution: 2.27→43.249 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 25.09

	R_factor	Num. reflection	% reflection
R_free	0.1997	5148	4.77 %
R_work	0.1723	-	-
obs	0.1737	107998	99.93 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Displacement parameters

B_iso max: 188.33 Å² / B_iso mean: 60.8634 Å² / B_iso min: 23.1 Å²

Refinement step

Cycle: final / Resolution: 2.27→43.249 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	11820	0	26	721	12567
B_iso mean	-	-	82.27	52.85	-
Num. residues	-	-	-	-	1454

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 30

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
2.27-2.2958	0.357	214	0.2882	3373	3587	100
2.2958-2.3228	0.2944	125	0.275	3485	3610	100
2.3228-2.3512	0.3271	161	0.2616	3461	3622	100
2.3512-2.3809	0.3186	168	0.2545	3414	3582	100
2.3809-2.4122	0.319	115	0.244	3489	3604	100
2.4122-2.4453	0.2602	164	0.2422	3440	3604	100
2.4453-2.4802	0.2489	148	0.227	3440	3588	100
2.4802-2.5172	0.2829	154	0.2267	3465	3619	100
2.5172-2.5566	0.2472	180	0.2183	3424	3604	100
2.5566-2.5985	0.2416	167	0.2147	3435	3602	100
2.5985-2.6433	0.2187	188	0.2054	3416	3604	100
2.6433-2.6913	0.2325	149	0.2008	3454	3603	100
2.6913-2.7431	0.2209	197	0.1971	3386	3583	100
2.7431-2.7991	0.2071	258	0.1844	3352	3610	100
2.7991-2.8599	0.2084	169	0.1791	3419	3588	100
2.8599-2.9264	0.1959	170	0.1764	3418	3588	100
2.9264-2.9996	0.2177	184	0.1762	3418	3602	100
2.9996-3.0807	0.1962	160	0.1749	3454	3614	100
3.0807-3.1713	0.2005	187	0.1679	3390	3577	100
3.1713-3.2736	0.1932	234	0.1694	3387	3621	100
3.2736-3.3906	0.1753	177	0.156	3444	3621	100
3.3906-3.5263	0.1575	166	0.1499	3432	3598	100
3.5263-3.6867	0.1803	150	0.1378	3456	3606	100
3.6867-3.8809	0.1523	135	0.1362	3426	3561	100
3.8809-4.1239	0.157	135	0.1355	3489	3624	100
4.1239-4.442	0.1483	144	0.1271	3443	3587	100
4.442-4.8885	0.1637	156	0.1358	3475	3631	100
4.8885-5.5946	0.1699	160	0.157	3420	3580	100
5.5946-7.0437	0.2222	210	0.1976	3393	3603	100
7.0437-43.2568	0.2121	223	0.1989	3352	3575	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.5415	1.665	-0.2032	3.3801	0.9291	4.7602	-0.0429	0.1502	-0.1694	-0.1621	0.1002	0.8674	0.0803	-1.1459	-0.0321	0.3817	0.0623	0.0675	0.7281	-0.0106	0.9811	-20.2776	29.9781	114.2512
2	2.2993	0.9688	-1.0296	3.3411	-0.7082	2.563	0.2822	0.2863	0.4489	-0.1515	-0.1289	0.9613	-0.5808	-0.4971	-0.1527	0.4802	0.2167	-0.0056	0.5607	0.0166	0.6899	-10.8098	44.2011	106.1243
3	3.1319	1.0961	-0.4538	4.3619	-0.0061	2.0651	0.0997	0.8955	0.4045	-0.6735	0.1072	0.5611	-0.4908	-0.354	-0.1579	0.6417	0.2048	-0.0636	0.6595	0.1725	0.4766	-1.3818	46.8673	93.6967
4	3.5554	-0.8908	-1.0063	4.0132	0.9818	3.5803	0.4401	0.531	-0.0602	-0.2178	-0.0348	1.0418	-0.5581	-1.09	0.041	0.5034	0.2101	-0.0906	0.7706	0.0932	1.0104	-19.4004	42.3104	103.8779
5	2.9473	0.0041	-1.0919	3.2844	1.3362	3.1697	0.4281	-0.0521	0.6171	-0.0637	-0.1996	1.0021	-0.7425	-0.2765	-0.1044	0.6575	0.2738	0.042	0.5561	0.1102	0.9183	-12.4591	53.4297	110.2738
6	4.3855	-0.3541	-0.9746	2.7529	0.4959	2.3511	0.0983	0.1712	0.6046	-0.401	-0.0561	0.5441	-0.264	-0.2131	-0.1351	0.4965	0.069	-0.0547	0.4357	0.0279	0.4038	1.3141	40.1223	99.5355
7	2.0633	1.5338	-0.6937	3.8109	-1.2687	4.6644	0.1668	0.0303	0.2188	-0.3881	-0.0218	-0.1234	-0.5378	0.3185	-0.17	0.537	-0.0533	0.0793	0.4724	-0.0146	0.298	30.3918	42.9328	97.8369
8	8.4931	3.1672	-1.2373	8.1593	-2.7753	6.8888	-0.1151	0.366	-0.3762	-0.7391	-0.2588	-0.8782	0.1557	0.9839	0.1665	0.4539	0.0158	0.0967	0.5981	-0.0306	0.3285	36.1835	34.9926	97.6821
9	7.1864	0.011	-0.8077	3.0141	-0.3842	5.9616	-0.2405	0.6486	-1.3344	-0.4487	0.0543	-0.2681	1.1169	-0.1606	0.1698	0.5775	0.0178	0.0102	0.4001	-0.1271	0.3802	20.979	18.9533	102.0915
10	1.0203	0.2394	-0.4718	1.7352	-0.3532	1.6466	0.0155	0.1998	-0.1451	-0.1291	-0.0374	0.4258	-0.0495	-0.3093	0.0116	0.3078	0.0192	-0.0384	0.4029	-0.0391	0.4089	-0.4362	26.7836	107.4472
11	1.1341	0.3139	-0.7857	1.3672	-0.3718	1.8114	0.1838	0.2948	0.2423	-0.3722	-0.0455	0.3891	-0.4761	-0.1817	-0.1275	0.5693	0.1061	-0.0975	0.53	0.0368	0.3475	5.4384	42.4172	91.168
12	0.9976	0.5323	-0.7226	2.3373	-0.2769	1.7287	-0.021	0.3347	-0.1646	-0.3434	-0.0052	0.1068	0.0155	-0.0723	0.0197	0.327	0.0309	-0.0489	0.3493	-0.0315	0.2428	14.1251	23.2679	99.8556
13	4.5521	0.4936	-0.1498	2.0361	0.8194	1.8533	-0.0008	-0.3878	-0.2694	0.5669	-0.0092	-0.1397	0.0973	0.2653	0.0221	0.5691	-0.0074	-0.036	0.3992	0.0453	0.2492	23.7319	24.846	128.0979
14	2.1189	2.0609	-1.3589	5.2393	-4.4775	9.4737	0.1824	-0.0808	-0.0559	0.4855	-0.0287	-0.3367	-1.0304	0.5353	-0.1789	0.4275	-0.0428	0.0027	0.4058	-0.09	0.2701	30.5402	37.7147	116.4375
15	3.7549	-0.1155	1.2802	3.4212	1.4019	9.0823	0.0553	-0.0791	-0.0459	0.2433	0.1464	-0.4684	-0.1031	0.9498	-0.2358	0.2959	-0.0457	-0.0092	0.3244	0.0268	0.2347	35.2499	31.4192	117.2746
16	1.8321	-0.2376	-0.0299	1.806	-0.1021	1.2409	0.0227	-0.1415	0.1653	0.3682	-0.0244	0.3863	-0.2168	-0.1446	0.0205	0.4642	0.002	0.1241	0.3561	-0.0172	0.3759	3.5779	34.682	122.5049
17	4.8035	-0.8098	0.7823	3.8782	0.4388	4.0762	-0.0238	-0.4276	-0.296	0.343	0.005	1.2242	-0.1287	-0.6864	0.0484	0.481	0.0007	0.2101	0.5618	-0.0088	0.7714	-12.897	31.6332	121.9193
18	2.7892	-2.0493	4.1767	2.9527	-1.3881	8.2343	0.1162	0.3376	0.6629	-0.0384	0.0137	-0.9369	-0.5111	1.0312	-0.2552	0.4349	-0.1605	0.0407	0.5196	-0.1173	0.9586	36.0608	102.5873	145.3327
19	2.7578	-1.1066	1.8635	3.3857	0.3926	1.9347	0.0653	0.4456	0.6215	-0.1028	-0.1715	-0.638	-0.2263	0.5607	0.0885	0.278	-0.1073	0.1147	0.6701	-0.0127	0.771	43.6947	87.3501	137.0065
20	4.9367	1.1703	3.8002	5.2888	1.6238	3.0294	-0.0596	-0.0096	-0.7401	0.3983	0.1371	-0.4855	0.6705	0.8833	-0.1084	0.4801	0.0956	0.071	0.7864	-0.081	0.7884	44.6963	68.6901	131.1299
21	6.2789	-3.9482	1.308	3.1893	-1.8166	2.4818	0.3901	0.9927	0.7637	-1.3837	-0.399	-1.7314	-0.1761	0.9668	0.2295	0.6543	-0.2258	0.2859	0.88	-0.0043	0.7338	38.5067	86.091	118.8685
22	2.7525	-1.7091	2.117	3.123	-1.3448	4.9757	-0.4061	0.4427	1.0522	-0.2007	0.1268	-0.8569	-0.6226	0.8617	0.2185	0.4442	-0.2265	0.1723	0.8041	-0.0061	1.0082	46.3419	95.7939	134.8119
23	1.9607	-1.7279	2.3196	4.095	-2.914	5.4916	-0.0646	0.0903	0.4883	-0.0961	-0.1127	-0.9065	0.3808	0.8424	0.0242	0.3976	-0.0605	0.0947	0.9345	-0.082	0.9051	52.5058	83.8814	141.3336
24	5.3239	3.7197	0.8699	9.57	0.119	3.782	0.034	0.4666	-0.2268	-0.3778	-0.1731	-0.5293	0.374	0.3452	0.1768	0.306	0.0629	0.0773	0.4807	-0.036	0.4557	34.1573	78.9593	130.3072
25	7.6091	7.1572	-4.0365	9.4876	-4.2619	7.255	-0.0202	0.3279	-0.4739	-0.567	0.085	-1.1332	0.7129	0.4566	-0.1154	0.4849	0.1038	0.0238	0.4484	-0.0964	0.547	22.5205	52.3615	128.4339
26	9.3708	-3.9406	-4.0667	3.1222	4.9706	9.4201	-0.3118	0.3446	-0.7379	-0.6778	-0.1346	-0.0876	0.5377	-0.526	0.4713	0.5806	-0.0056	0.0822	0.3637	0.0071	0.5183	12.8413	51.0588	128.1923
27	2.9366	1.0906	0.1795	6.0987	0.0007	5.6077	0.2607	0.4988	0.3906	-0.7185	-0.3749	0.6321	-0.4845	-0.5993	0.0931	0.3909	0.0556	-0.0176	0.3851	-0.0174	0.4431	5.9276	72.3296	132.6565
28	1.1927	-0.3718	-0.3038	1.5571	0.7935	1.6758	-0.0559	0.0633	0.2461	-0.1686	0.0471	-0.2508	-0.1829	0.2843	-0.0513	0.3097	-0.0262	0.0206	0.3181	0.0441	0.5023	23.434	87.0006	138.288
29	0.8202	-0.1155	-0.073	1.5436	1.2526	1.9628	-0.0292	0.2153	0.0241	-0.2745	0.2755	-0.4895	0.0549	0.6675	-0.1371	0.4143	-0.0108	0.1346	0.5691	0.0005	0.5427	34.1739	74.0123	121.8773
30	1.1662	-0.0666	0.2918	1.1116	0.2831	1.1506	-0.0561	0.0822	-0.0397	-0.1323	0.0222	-0.0328	0.0321	-0.0145	0.0351	0.2771	0.0047	0.037	0.2495	0.0206	0.3985	12.2081	74.6647	136.1728
31	2.9999	0.1921	0.523	2.6422	1.1353	4.3762	0.0085	-0.5235	-0.4548	0.5855	0.0339	0.1189	0.4563	-0.4006	0.0581	0.4134	0.0825	0.0389	0.4334	0.1449	0.402	10.0144	63.6931	163.7833
32	1.1816	-0.5461	-0.3892	1.3566	0.7986	4.6885	-0.0022	-0.1463	-0.4751	0.0565	-0.0348	-0.0197	0.797	0.0088	-0.0165	0.5071	0.027	0.0179	0.3014	0.079	0.5807	13.2212	54.0233	147.3964
33	1.8156	0.6403	-0.0589	2.3405	-0.1322	1.9929	-0.0551	-0.3389	-0.1048	0.1778	0.0227	-0.5088	0.2196	0.4844	0.0653	0.2843	0.055	-0.0354	0.4232	-0.0239	0.4847	28.2424	79.4624	153.2395
34	5.8427	0.9521	-0.588	3.3374	-0.4989	2.4653	-0.0982	-0.459	1.0215	0.3205	0.1447	-0.3877	-0.7501	0.7258	-0.0229	0.5042	-0.0784	-0.0944	0.5743	-0.1437	0.7452	33.8272	95.2749	152.8968

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 7 through 25 )	A	7 - 25
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 26 through 61 )	A	26 - 61
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 62 through 89 )	A	62 - 89
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 90 through 117 )	A	90 - 117
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 118 through 133 )	A	118 - 133
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 134 through 152 )	A	134 - 152
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 153 through 168 )	A	153 - 168
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 169 through 180 )	A	169 - 180
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 181 through 197 )	A	181 - 197
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 1 through 81 )	B	1 - 81
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 82 through 187 )	B	82 - 187
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 188 through 281 )	B	188 - 281
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 282 through 345 )	B	282 - 345
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 346 through 378 )	B	346 - 378
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 379 through 424 )	B	379 - 424
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 425 through 493 )	B	425 - 493
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 494 through 536 )	B	494 - 536
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 7 through 25 )	C	7 - 25
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 26 through 61 )	C	26 - 61
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 62 through 74 )	C	62 - 74
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 75 through 89 )	C	75 - 89
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 90 through 117 )	C	90 - 117
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 118 through 133 )	C	118 - 133
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 134 through 152 )	C	134 - 152
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 153 through 168 )	C	153 - 168
26	X-RAY DIFFRACTION	26	chain 'C' and (resid 169 through 180 )	C	169 - 180
27	X-RAY DIFFRACTION	27	chain 'C' and (resid 181 through 197 )	C	181 - 197
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 1 through 81 )	D	1 - 81
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 82 through 187 )	D	82 - 187
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 188 through 312 )	D	188 - 312
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 313 through 345 )	D	313 - 345
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 346 through 424 )	D	346 - 424
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 425 through 493 )	D	425 - 493
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 494 through 536 )	D	494 - 536

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