Mass: 46222.512 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Mismatches are methylated Valine, methylated Isoleucine, methylated Glycine. The enzyme has a peptide sequence at the C-terminus that is not methylated until it is recombinantly expressed (sequence VISSVVA) Source: (gene. exp.) Dendrothele bispora CBS 962.96 (fungus) Production host: Escherichia coli (E. coli) / References: UniProt: A0A452CSY9*PLUS
Mass: 18.015 Da / Num. of mol.: 187 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.44 Å3/Da / Density % sol: 49.52 %
Crystal grow
Temperature: 282.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 2 mM SAH mixed 0.24 or 0.26 M NaF, 26% PEG3350, and 0.1 M Bis-tris propane at pH 6.5
Method to determine structure: SAD / Resolution: 2.123→25 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.94 / SU B: 6.102 / SU ML: 0.151 / Cross valid method: THROUGHOUT / ESU R: 0.219 / ESU R Free: 0.182 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22701
1216
4.8 %
RANDOM
Rwork
0.18621
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obs
0.18833
23975
96.15 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å