+Open data
-Basic information
Entry | Database: PDB / ID: 6kzg | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | 14-3-3 protein in Complex with CIC S301 phosphorylated peptide | ||||||||||||
Components |
| ||||||||||||
Keywords | SIGNALING PROTEIN / 14-3-3 protein / CIC / phosphorylation / transcriptional regulation | ||||||||||||
Function / homology | Function and homology information negative regulation of monoatomic ion transmembrane transport / small GTPase-mediated signal transduction / social behavior / Regulation of localization of FOXO transcription factors / protein targeting / Activation of BAD and translocation to mitochondria / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs ...negative regulation of monoatomic ion transmembrane transport / small GTPase-mediated signal transduction / social behavior / Regulation of localization of FOXO transcription factors / protein targeting / Activation of BAD and translocation to mitochondria / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs / substantia nigra development / 14-3-3 protein binding / learning / Translocation of SLC2A4 (GLUT4) to the plasma membrane / TP53 Regulates Metabolic Genes / RNA polymerase II transcription regulatory region sequence-specific DNA binding / brain development / memory / transmembrane transporter binding / DNA-binding transcription factor activity, RNA polymerase II-specific / protein domain specific binding / focal adhesion / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / synapse / chromatin / regulation of transcription by RNA polymerase II / signal transduction / protein-containing complex / extracellular exosome / nucleoplasm / membrane / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||||||||
Authors | Wen, Y. / Shao, Y. | ||||||||||||
Funding support | China, 3items
| ||||||||||||
Citation | Journal: To Be Published Title: 14-3-3 protein in Complex with CIC S301 phosphorylated peptide Authors: Wen, Y. / Shao, Y. | ||||||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6kzg.cif.gz | 208.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6kzg.ent.gz | 165.7 KB | Display | PDB format |
PDBx/mmJSON format | 6kzg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kz/6kzg ftp://data.pdbj.org/pub/pdb/validation_reports/kz/6kzg | HTTPS FTP |
---|
-Related structure data
Related structure data | 5iqpS S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 29857.580 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: YWHAQ / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P27348 #2: Protein/peptide | Mass: 948.913 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CIC, KIAA0306 / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q96RK0 #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.61 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 8% v/v TacsimateTM pH 6.0, 20% w/v Polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.98 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 26, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2→44.17 Å / Num. obs: 40758 / % possible obs: 99.81 % / Redundancy: 12.8 % / CC1/2: 1 / Rmerge(I) obs: 0.113 / Net I/σ(I): 16.9 |
Reflection shell | Resolution: 2→2.07 Å / Num. unique obs: 3950 / CC1/2: 0.61 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5IQP Resolution: 2→44.17 Å / Cross valid method: THROUGHOUT
| ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 129.51 Å2 / Biso mean: 52.403 Å2 / Biso min: 20 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→44.17 Å
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Origin x: -69.3197 Å / Origin y: -0.6816 Å / Origin z: -2.95 Å
| ||||||||||||||||||||||||||||||||||||||||
Refinement TLS group | Selection details: all |