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- PDB-6jh8: Crystal structure of an actin monomer in complex with a chimeric ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jh8 | ||||||
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Title | Crystal structure of an actin monomer in complex with a chimeric peptide of Cordon-Bleu WH2 mutant and MIM. | ||||||
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Function / homology | ![]() somite specification / floor plate development / actin filament network formation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Scipion, C.P.M. / Robinson, R.C. | ||||||
![]() | ![]() Title: Design of an actin-severing peptide. Authors: Scipion, C.P.M. / Robinson, R.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.7 KB | Display | ![]() |
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PDB format | ![]() | 73 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6jcuC ![]() 6jh9C ![]() 5ypuS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 42067.871 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() ![]() | ||
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#2: Protein/peptide | Mass: 3232.653 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() | ||
#3: Chemical | ChemComp-ATP / ![]() | ||
#4: Chemical | #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.39 % |
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Crystal grow![]() | Temperature: 298.15 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 0.1M sodium acetate trihydrate, pH 4.6, 10% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 23, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.149→42.47 Å / Num. obs: 22798 / % possible obs: 99.2 % / Redundancy: 3.7 % / CC1/2: 0.995 / Rmerge(I) obs: 0.124 / Rpim(I) all: 0.075 / Rrim(I) all: 0.145 / Net I/σ(I): 7.3 |
Reflection shell | Resolution: 2.149→2.21 Å / Redundancy: 3.7 % / Num. unique obs: 1810 / CC1/2: 0.702 / Rpim(I) all: 0.524 / % possible all: 96.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5YPU Resolution: 2.149→42.467 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.86
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.149→42.467 Å
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Refine LS restraints |
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LS refinement shell |
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