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- PDB-6hf1: Mutant oxidoreductase fragment of mouse QSOX1 in complex with an ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6hf1 | ||||||
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Title | Mutant oxidoreductase fragment of mouse QSOX1 in complex with an antibody Fab | ||||||
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Function / homology | ![]() flavin-dependent sulfhydryl oxidase activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Grossman-Haham, I. / Fass, D. | ||||||
Funding support | 1items
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![]() | ![]() Title: cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures. Authors: Javitt, G. / Grossman-Haham, I. / Alon, A. / Resnick, E. / Mutsafi, Y. / Ilani, T. / Fass, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 281.7 KB | Display | ![]() |
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PDB format | ![]() | 223.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5d93S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 26326.746 Da / Num. of mol.: 2 / Mutation: C75A, P122T Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Antibody | | Mass: 23108.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #3: Antibody | Mass: 23087.605 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #4: Antibody | | Mass: 23237.914 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 50.06 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 0.1 M sodium citrate, pH 6.0 16% w/v PEG |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Feb 16, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.937→98 Å / Num. obs: 104667 / % possible obs: 97.6 % / Redundancy: 2.3 % / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 1.94→2.02 Å / Redundancy: 2.3 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 8728 / % possible all: 82.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5D93 Resolution: 1.94→48.886 Å / SU ML: 0.24 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 25.09
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.94→48.886 Å
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Refine LS restraints |
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LS refinement shell |
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