Mass: 21496.318 Da / Num. of mol.: 2 / Mutation: Del112-199 Source method: isolated from a genetically manipulated source Details: No density was visible for the residue regions 1 to 11 and 78 to 111. Source: (gene. exp.) Homo sapiens (human) / Gene: SSSCA1 Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: O60232
Resolution: 2.3→2.36 Å / Redundancy: 6.9 % / Mean I/σ(I) obs: 1.4 / Num. unique obs: 701 / Rpim(I) all: 0.631 / % possible all: 98.5
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0230
refinement
XDS
datareduction
SCALA
datascaling
PHASER
phasing
Refinement
Method to determine structure: SAD / Resolution: 2.3→25 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.955 / SU B: 18.719 / SU ML: 0.203 / Cross valid method: THROUGHOUT / ESU R: 0.243 / ESU R Free: 0.209 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2603
483
5 %
RANDOM
Rwork
0.22151
-
-
-
obs
0.22354
9179
99.18 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK