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- PDB-6h7o: ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND WEAK PARTIAL AGONI... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6h7o | ||||||
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Title | ACTIVATED TURKEY BETA1 ADRENOCEPTOR WITH BOUND WEAK PARTIAL AGONIST CYANOPINDOLOL AND NANOBODY Nb6B9 | ||||||
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Function / homology | ![]() beta1-adrenergic receptor activity / positive regulation of heart contraction / regulation of circadian sleep/wake cycle, sleep / DNA polymerase processivity factor activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Warne, T. / Edwards, P.C. / Dore, A.S. / Leslie, A.G.W. / Tate, C.G. | ||||||
![]() | ![]() Title: Molecular basis for high-affinity agonist binding in GPCRs. Authors: Warne, T. / Edwards, P.C. / Dore, A.S. / Leslie, A.G.W. / Tate, C.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 217.4 KB | Display | ![]() |
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PDB format | ![]() | 172.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6h7jC ![]() 6h7lC ![]() 6h7mC ![]() 2h6xS ![]() 3p0gS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 0 / Refine code: 0
NCS ensembles :
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Components
-Protein , 2 types, 4 molecules EFAB
#1: Protein | ![]() Mass: 11784.370 Da / Num. of mol.: 2 / Mutation: C32S,C35S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: trxA, fipA, tsnC, b3781, JW5856 / Production host: ![]() ![]() #2: Protein | ![]() Mass: 35002.777 Da / Num. of mol.: 2 / Mutation: R68S,M90V,C116L,F327A,F338M,C358A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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-Antibody , 1 types, 2 molecules CD
#3: Antibody | Mass: 13036.453 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Non-polymers , 4 types, 18 molecules ![](data/chem/img/P32.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/2CV.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/2CV.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | #6: Chemical | ChemComp-2CV / #7: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 67.64 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1 M Hepes-NaOH pH7.5 and 21-24% PEG1500 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Nov 12, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.79→38.22 Å / Num. obs: 45172 / % possible obs: 98.3 % / Redundancy: 9.8 % / Net I/σ(I): 3.7 |
Reflection shell | Resolution: 2.79→2.89 Å |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2H6X,3P0G Resolution: 2.8→38.22 Å / Cor.coef. Fo:Fc: 0.867 / Cor.coef. Fo:Fc free: 0.769 / SU B: 20.948 / SU ML: 0.386 / Cross valid method: THROUGHOUT / ESU R Free: 0.581 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 80.741 Å2
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Refinement step | Cycle: 1 / Resolution: 2.8→38.22 Å
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Refine LS restraints |
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