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- PDB-6h18: Crystal structure of sarin surrogate NIMP inhibited recombinant h... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6h18 | |||||||||
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Title | Crystal structure of sarin surrogate NIMP inhibited recombinant human bile salt activated lipase | |||||||||
![]() | Bile salt-activated lipase | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Touvrey, C. / Brazzolotto, X. / Nachon, F. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: X-ray structures of human bile-salt activated lipase conjugated to nerve agents surrogates. Authors: Touvrey, C. / Courageux, C. / Guillon, V. / Terreux, R. / Nachon, F. / Brazzolotto, X. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 126.2 KB | Display | ![]() |
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PDB format | ![]() | 93.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6h0tC ![]() 6h0vC ![]() 6h19C ![]() 6h1aC ![]() 1f6wS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 60862.801 Da / Num. of mol.: 1 / Mutation: N186D, A298D, 534STOP Source method: isolated from a genetically manipulated source Details: SGB = serine residue inhibited by sarin (see http://www.rcsb.org/ligand/SGB) Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P19835, ![]() ![]() | ||||
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#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-ACT / ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.56 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG 8000, cacodylate, Zinc Acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 15, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.85→48.43 Å / Num. obs: 52277 / % possible obs: 99.16 % / Redundancy: 4 % / CC1/2: 0.998 / Rmerge(I) obs: 0.09432 / Rpim(I) all: 0.05251 / Rrim(I) all: 0.1086 / Net I/σ(I): 7.88 |
Reflection shell | Resolution: 1.85→1.916 Å / Redundancy: 4.1 % / Num. unique obs: 5101 / % possible all: 98.49 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1f6w Resolution: 1.85→48.43 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.85→48.43 Å
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