Mass: 18.015 Da / Num. of mol.: 71 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.3 Å3/Da / Density % sol: 46.55 %
Crystal grow
Temperature: 291 K / Method: vapor diffusion, hanging drop Details: Hanging drop vapor diffusion method; mixture of 4 uL 3.9 mg/mL protein solution, 2 uL of precipitating solution (0.2 M MgCl2, 0.1 M Tris pH 7, 30%(w/v) PEG 4000) and 1uL of additive solution ...Details: Hanging drop vapor diffusion method; mixture of 4 uL 3.9 mg/mL protein solution, 2 uL of precipitating solution (0.2 M MgCl2, 0.1 M Tris pH 7, 30%(w/v) PEG 4000) and 1uL of additive solution (0.6 M MgCl2) at 291 K.
Resolution: 2.343→30.28 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.895 / SU B: 15.166 / SU ML: 0.331 / Cross valid method: THROUGHOUT / ESU R: 0.485 / ESU R Free: 0.298 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.27845
891
5.1 %
RANDOM
Rwork
0.21126
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obs
0.21463
16605
96.06 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å