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Yorodumi- PDB-6fll: SPECTROSCOPIC AND STRUCTURAL STUDY OF QW, A EGFP MUTANT SHOWING P... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6fll | |||||||||
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Title | SPECTROSCOPIC AND STRUCTURAL STUDY OF QW, A EGFP MUTANT SHOWING PHOTOSWITCHING PROPERTIES | |||||||||
Components | Green fluorescent protein | |||||||||
Keywords | FLUORESCENT PROTEIN / GFP / EGFP / LUMINESCENCE / FLUORESCENT / PROTEIN / BIOLUMINESCENCE / PHOTOACTIVE / CHROMOPHORE | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Aequorea victoria (jellyfish) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.79 Å | |||||||||
Authors | Garau, G. / Margheritis, E. | |||||||||
Funding support | Italy, 1items
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Citation | Journal: Acs Chem.Biol. / Year: 2018 Title: Role of Gln222 in Photoswitching of Aequorea Fluorescent Proteins: A Twisting and H-Bonding Affair? Authors: Storti, B. / Margheritis, E. / Abbandonato, G. / Domenichini, G. / Dreier, J. / Testa, I. / Garau, G. / Nifosi, R. / Bizzarri, R. #1: Journal: To Be Published Title: STRUCTURE OF THE GREEN FLUORESCENCE PROTEIN WQ Authors: Garau, G. / Margheritis, E. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6fll.cif.gz | 63.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6fll.ent.gz | 43.3 KB | Display | PDB format |
PDBx/mmJSON format | 6fll.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fl/6fll ftp://data.pdbj.org/pub/pdb/validation_reports/fl/6fll | HTTPS FTP |
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-Related structure data
Related structure data | 2o24S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28554.172 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aequorea victoria (jellyfish) / Gene: GFP / Production host: Escherichia coli (E. coli) / References: UniProt: P42212 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.82 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5 Details: PEG 3350, 0.1 M AMMONIUM ACETATE, 0.2 M NH4F, PH 5.0 PH range: 5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ELETTRA / Beamline: 5.2R / Wavelength: 0.96 Å |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Oct 10, 2017 |
Radiation | Monochromator: SI111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96 Å / Relative weight: 1 |
Reflection | Resolution: 1.79→66.13 Å / Num. obs: 20947 / % possible obs: 100 % / Redundancy: 6 % / Rmerge(I) obs: 0.183 / Rsym value: 0.12 / Net I/σ(I): 7.4 |
Reflection shell | Resolution: 1.79→1.82 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.766 / Mean I/σ(I) obs: 1.4 / Rsym value: 0.761 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2O24 Resolution: 1.79→45.5 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.928 / SU B: 3.555 / SU ML: 0.106 / Cross valid method: THROUGHOUT / ESU R: 0.147 / ESU R Free: 0.132 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.796 Å2
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Refinement step | Cycle: LAST / Resolution: 1.79→45.5 Å
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Refine LS restraints |
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