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- PDB-6f4h: Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex -
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Open data
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Basic information
Entry | Database: PDB / ID: 6f4h | ||||||
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Title | Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex | ||||||
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Function / homology | ![]() primary sex determination, soma / snRNA stem-loop binding / pre-mRNA intronic pyrimidine-rich binding / mRNA Splicing - Major Pathway / female germ-line sex determination / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Weber, G. / Holton, N. / Hall, K.B. / DeKoster, G. / Wahl, M.C. | ||||||
![]() | ![]() Title: Molecular principles underlying dual RNA specificity in the Drosophila SNF protein. Authors: Weber, G. / DeKoster, G.T. / Holton, N. / Hall, K.B. / Wahl, M.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.3 KB | Display | ![]() |
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PDB format | ![]() | 86.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 11082.006 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: RNA chain | Mass: 7000.209 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() ![]() #3: Chemical | ChemComp-FLC / | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.05 % |
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Crystal grow![]() | Temperature: 277 K / Method: batch mode Details: 0.2 M tri-lithium citrate, 20 % (w/v) PEG 3350 and 15 mM NiCl2 as an additive |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Jul 18, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2→50 Å / Num. obs: 39021 / % possible obs: 99.7 % / Redundancy: 10.9 % / Rsym value: 0.11 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 2→2.12 Å |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→38.302 Å
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Refine LS restraints |
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LS refinement shell |
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