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- PDB-6eyo: Structure of extended IgE-Fc in complex with two anti-IgE Fabs -

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Basic information

Entry
Database: PDB / ID: 6eyo
TitleStructure of extended IgE-Fc in complex with two anti-IgE Fabs
Components
  • 8D6 Fab heavy chain
  • 8D6 Fab light chain
  • Immunoglobulin heavy constant epsilon
KeywordsIMMUNE SYSTEM / Immunoglobulin E / Fab / antibody / complex
Function / homology
Function and homology information


IgE B cell receptor complex / adaptive immune memory response / primary adaptive immune response / type I hypersensitivity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / eosinophil degranulation / IgE immunoglobulin complex / macrophage activation / type 2 immune response ...IgE B cell receptor complex / adaptive immune memory response / primary adaptive immune response / type I hypersensitivity / B cell antigen processing and presentation / Fc receptor-mediated immune complex endocytosis / eosinophil degranulation / IgE immunoglobulin complex / macrophage activation / type 2 immune response / Fc epsilon receptor (FCERI) signaling / antibody-dependent cellular cytotoxicity / mast cell degranulation / B cell proliferation / macrophage differentiation / Role of LAT2/NTAL/LAB on calcium mobilization / immunoglobulin complex, circulating / immunoglobulin receptor binding / FCERI mediated Ca+2 mobilization / complement activation, classical pathway / antigen binding / FCERI mediated MAPK activation / B cell receptor signaling pathway / FCERI mediated NF-kB activation / antibacterial humoral response / Interleukin-4 and Interleukin-13 signaling / adaptive immune response / inflammatory response / immune response / extracellular space / extracellular region / plasma membrane
Similarity search - Function
Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold ...Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Immunoglobulin heavy constant epsilon
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.7 Å
AuthorsChen, J.B. / Ramadani, F. / Pang, M.O.Y. / Beavil, R.L. / Holdom, M.D. / Mitropoulou, A.N. / Beavil, A.J. / Gould, H.J. / Chang, T.W. / Sutton, B.J. ...Chen, J.B. / Ramadani, F. / Pang, M.O.Y. / Beavil, R.L. / Holdom, M.D. / Mitropoulou, A.N. / Beavil, A.J. / Gould, H.J. / Chang, T.W. / Sutton, B.J. / McDonnell, J.M. / Davies, A.M.
Funding support United Kingdom, 3items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)G1100090 United Kingdom
Asthma UKAUK-PG-2013-183 United Kingdom
Wellcome Trust085944 United Kingdom
CitationJournal: Sci Rep / Year: 2018
Title: Structural basis for selective inhibition of immunoglobulin E-receptor interactions by an anti-IgE antibody.
Authors: Chen, J.B. / Ramadani, F. / Pang, M.O.Y. / Beavil, R.L. / Holdom, M.D. / Mitropoulou, A.N. / Beavil, A.J. / Gould, H.J. / Chang, T.W. / Sutton, B.J. / McDonnell, J.M. / Davies, A.M.
History
DepositionNov 13, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 15, 2018Provider: repository / Type: Initial release
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_entity_id / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Jan 17, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Immunoglobulin heavy constant epsilon
B: Immunoglobulin heavy constant epsilon
I: 8D6 Fab heavy chain
H: 8D6 Fab heavy chain
M: 8D6 Fab light chain
L: 8D6 Fab light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)171,7358
Polymers169,4276
Non-polymers2,3082
Water0
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18740 Å2
ΔGint-44 kcal/mol
Surface area66380 Å2
MethodPISA
Unit cell
Length a, b, c (Å)119.483, 119.601, 132.994
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Immunoglobulin heavy constant epsilon / Ig epsilon chain C region / Ig epsilon chain C region ND


Mass: 36354.781 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: Fc region of immunoglobulin E / Source: (gene. exp.) Homo sapiens (human) / Gene: IGHE / Cell line (production host): MOUSE MYELOMA NS0 / Production host: Mus musculus (house mouse) / References: UniProt: P01854
#2: Antibody 8D6 Fab heavy chain


Mass: 24555.291 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: 8D6 Fab heavy chain / Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody 8D6 Fab light chain


Mass: 23803.211 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: 8D6 Fab light chain / Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1235.105 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-3[DManpa1-3DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,7,6/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-f1_d2-e1_f3-g1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#5: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1072.964 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3/a4-b1_b4-c1_c3-d1_c6-f1_d2-e1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3 Å3/Da / Density % sol: 58.94 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8
Details: 0.1M Tris-HCl pH8.0 and 45% (v/v) pentaerythritol propoxylate (5/4PO/OH)

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Feb 23, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.92 Å / Relative weight: 1
ReflectionResolution: 3.7→88.93 Å / Num. obs: 20732 / % possible obs: 98.8 % / Redundancy: 6.6 % / CC1/2: 0.989 / Rmerge(I) obs: 0.224 / Rpim(I) all: 0.094 / Net I/σ(I): 6.8
Reflection shellResolution: 3.7→4.05 Å / Redundancy: 6.8 % / Rmerge(I) obs: 0.691 / Mean I/σ(I) obs: 2.8 / Num. unique obs: 4698 / CC1/2: 0.819 / Rpim(I) all: 0.284 / % possible all: 95.3

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Processing

Software
NameVersionClassification
PHENIX(1.10_2155: ???)refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2wqr

2wqr


Resolution: 3.7→88.93 Å / SU ML: 0.49 / Cross valid method: THROUGHOUT / σ(F): 1.25 / Phase error: 29.32
RfactorNum. reflection% reflection
Rfree0.2882 1125 5.44 %
Rwork0.2473 --
obs0.2496 20683 98.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 3.7→88.93 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10230 0 155 0 10385
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00210674
X-RAY DIFFRACTIONf_angle_d0.5114687
X-RAY DIFFRACTIONf_dihedral_angle_d11.5416261
X-RAY DIFFRACTIONf_chiral_restr0.041748
X-RAY DIFFRACTIONf_plane_restr0.0051866
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.7001-3.86850.34421080.3232220X-RAY DIFFRACTION91
3.8685-4.07240.33491510.28582413X-RAY DIFFRACTION100
4.0724-4.32760.33291330.26052464X-RAY DIFFRACTION100
4.3276-4.66170.27641400.22452432X-RAY DIFFRACTION100
4.6617-5.13080.24911520.22682447X-RAY DIFFRACTION100
5.1308-5.87310.25691420.23352455X-RAY DIFFRACTION100
5.8731-7.39890.31031430.2592522X-RAY DIFFRACTION100
7.3989-88.95550.27641560.23122605X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0723-0.0114-0.00050.04620.0047-0.00380.01290.02060.093-0.0307-0.0062-0.0049-0.0542-0.0313-0.03250.20620.28240.05470.06010.05230.069814.4946-60.332819.3387
20.04630.05470.07260.05590.07670.09890.11160.0822-0.17370.016-0.0108-0.068-0.1352-0.04640.01980.22270.02270.06230.34350.1380.3655-16.9842-84.911622.2831
30.07930.0195-0.04210.02730.01660.047-0.0292-0.0856-0.1008-0.4469-0.02450.26880.1592-0.1648-0.00010.5770.1513-0.0250.68830.18290.701-23.181-91.989517.1848
40.021-0.0202-0.02750.03270.02870.026-0.0541-0.2957-0.0892-0.1863-0.015-0.0027-0.0087-0.2038-00.43410.01260.03810.58980.06140.4644-30.0893-109.080920.3943
50.2847-0.1277-0.12260.10390.11360.11720.07280.00120.0921-0.02180.0843-0.074-0.05430.13990.4189-0.01120.29920.11890.2735-0.22930.06727.238-76.200916.2891
60.1023-0.01810.03450.0604-0.07420.1174-0.1091-0.0896-0.22790.22060.2394-0.04290.1148-0.07690.32250.40070.1428-0.16440.32690.01080.6167-5.2054-114.98029.7936
70.00220.0012-0.00010.0005-0.00120.0008-0.0042-0.0850.03630.0102-0.0518-0.1177-0.0320.1267-0.08620.2154-0.21550.05210.5471-0.03450.24924.7898-82.4511-13.0605
80.010.00880.00670.00510.00530.0057-0.00180.0206-0.0143-0.01710.0133-0.00360.0120.0324-0.06450.14860.1528-0.08670.4374-0.28680.3077-0.2347-91.0356-11.4042
90.22550.0637-0.20170.4609-0.16360.3174-0.1182-0.0069-0.14670.0692-0.1239-0.06710.03220.058-0.40930.1414-0.06550.08590.4425-0.07410.22380.4018-78.3586-19.4836
100.01430.00650.00290.0143-0.01160.0134-0.2192-0.05840.1711-0.0476-0.0897-0.1098-0.12590.128301.2841-0.14320.01540.5435-0.02990.9633-2.5446-59.8278-27.2393
110.00080.0030.00180.01990.0090.0044-0.03350.02520.0209-0.05410.0288-0.0018-0.0480.0296-0.00910.5499-0.2566-0.02040.3759-0.16680.39773.1496-83.129656.11
120.09040.01730.03930.00920.00310.0195-0.0716-0.05490.02370.04760.1092-0.0344-0.0118-0.04690.02770.3489-0.0466-0.02190.3349-0.0560.09181.9893-83.533442.6327
130.00770.00270.01010.0030.00070.02250.0263-0.00450.02050.0010.03060.0038-0.0213-0.04510.1150.0379-0.02870.04150.09360.07970.2341-1.3965-95.323448.9959
140.20190.0580.18850.1546-0.0360.23390.1522-0.0281-0.04310.0684-0.04330.0631-0.0008-0.02010.08240.26270.0123-0.03520.1867-0.16390.3778-7.0959-83.932643.7668
15-0.001-0.000700.0015-0.00330.00210.0164-0.0210.0614-0.0482-0.0035-0.031-0.0243-0.004800.58850.0462-0.01620.65450.12480.4936-4.473-86.062556.0724
160.00610.00580.00220.009-0.00360.00760.0226-0.00790.18150.0843-0.0404-0.0980.00050.08760.00010.4070.01850.01040.35880.1290.39764.6963-90.216238.9764
170.00060.0022-0.00230.0029-0.0010.0021-0.0307-0.05610.03540.0220.0143-0.030.0013-0.053200.8607-0.11570.0520.61140.07120.6145.23-85.753964.2743
180.00320.00040.0010.00310.00570.0078-0.0655-0.02640.06880.0492-0.15940.0447-0.0454-0.0203-00.8349-0.2069-0.20710.6814-0.24361.101825.9227-90.282672.1335
190.002-0.00080.0005-0.00150.0001-0.0008-0.00890.0091-0.00820.060.027-0.0643-0.04450.0019-01.04440.1016-0.22260.74550.19430.786821.7333-88.238262.3398
200.01-0.0083-0.00090.0035-0.0050.001-0.061-0.06880.07740.0793-0.05560.1483-0.0277-0.0926-01.0836-0.3045-0.08850.61940.00610.896818.7028-89.666968.1212
210.0021-0.0020.00070.0005-0.0004-0.00040.04560.02040.00190.00850.026-0.0319-0.0253-0.0224-01.11350.1715-0.11441.02950.04921.020527.2723-82.140871.2929
220.03280.0110.02510.00280.00650.02770.03140.0706-0.061-0.02240.04640.01340.0482-0.0570.02960.15420.1243-0.16770.2539-0.05340.3363-24.031-81.5203-9.7163
230.5122-0.1750.31950.0596-0.11060.2071-0.1115-0.05670.10240.0534-0.0780.05430.0761-0.1463-0.32650.14370.01750.10320.4133-0.13870.2841-16.0422-82.1561-1.7002
240.0530.00260.02460.00980.00630.0348-0.0263-0.02950.0423-0.0096-0.04530.0218-0.0372-0.0531-0.12480.3041-0.1356-0.25320.29360.06640.5231-15.6544-81.3878-7.8904
250.0030.00330.00610.00230.00410.00690.04430.0214-0.01460.01420.07740.00730.03680.000300.991-0.1039-0.22060.5166-0.03630.6517-25.6374-63.2807-17.2843
260.0067-0.00550.00140.0054-0.00470.0061-0.0143-0.01780.0237-0.0008-0.0285-0.0366-0.00240.0238-01.242-0.0963-0.05680.87540.18790.8217-9.8912-53.0745-38.2326
270.0129-0.00940.01420.0033-0.0080.0143-0.0161-0.0092-0.0557-0.04690.02170.0181-0.0553-0.017-0.0281.10190.1833-0.59070.866-0.16360.7571-20.5758-60.9001-33.6352
280.00090.00080.00080.0022-0.0020.0035-0.0003-0.01590.0109-0.00880.0103-0.00450.0318-0.0107-00.8245-0.0038-0.15490.48540.20820.5534-18.1809-63.1271-17.7368
290.00720.0025-0.00370.00360.00340.00610.05970.12930.013-0.03860.05360.0077-0.0477-0.0448-0.00011.0159-0.208-0.10260.90810.19960.8775-14.153-58.7685-41.1597
300.0103-0.01040.010.0098-0.010.0094-0.044-0.06110.0378-0.0261-0.0491-0.0413-0.0676-0.0502-0.03620.9480.1997-0.12530.81870.15960.8206-24.1798-52.8331-35.8112
310.0032-0.00620.00060.00810.001-0.0005-0.0963-0.0142-0.0447-0.0332-0.1129-0.03790.1566-0.074700.3862-0.0323-0.07060.45830.09690.70769.1941-111.867344.1519
320.0279-0.02660.00520.08860.06410.07950.075-0.0344-0.0182-0.0637-0.07610.00420.0926-0.0099-0.07310.2971-0.01420.0140.36440.25060.39235.1566-101.389638.3451
330.0017-0.0002-0.00250.0010.00060.0026-0.06810.0296-0.06090.0036-0.0059-0.02160.0643-0.008400.9460.04530.23380.86170.31260.81315.3199-101.238333.2715
340.0048-0.00510.00280.0071-0.00490.00180.06610.0344-0.0223-0.02280.0627-0.02980.03860.0779-00.67540.0889-0.04140.48220.03590.85239.2227-110.313935.5899
350.0342-0.0832-0.10510.20590.25870.3271-0.0702-0.0115-0.02240.1569-0.2615-0.0685-0.04030.0641-0.13540.225-0.0204-0.09550.41420.21470.619711.7016-105.159245.8727
36-0.00060.0001-0.00050.0019-0.00270.0033-0.016-0.0092-0.0451-0.0296-0.015-0.04610.0168-0.010601.1443-0.0362-0.45610.6540.05380.6726.9111-95.959377.1556
370.061-0.01330.04590.04920.06190.14390.05410.01420.10570.04630.0033-0.0612-0.0147-0.03720.07150.746-0.0501-0.19860.48890.16650.82523.218-106.47668.1207
380.0054-0.0059-0.0040.01190.00110.0042-0.033-0.02460.04480.01220.02630.04550.0115-0.00560.02590.9781-0.2332-0.26040.6220.09190.603922.958-99.66283.0409
390.0206-0.01130.01230.0111-0.00920.00770.0177-0.01940.0348-0.0314-0.0512-0.00160.00160.07840.02560.8929-0.0437-0.49630.51780.07720.683631.9619-106.973777.7789
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 231 through 326 )
2X-RAY DIFFRACTION2chain 'A' and (resid 327 through 396 )
3X-RAY DIFFRACTION3chain 'A' and (resid 397 through 458 )
4X-RAY DIFFRACTION4chain 'A' and (resid 459 through 544 )
5X-RAY DIFFRACTION5chain 'B' and (resid 231 through 337 )
6X-RAY DIFFRACTION6chain 'B' and (resid 338 through 544 )
7X-RAY DIFFRACTION7chain 'I' and (resid 2 through 40 )
8X-RAY DIFFRACTION8chain 'I' and (resid 41 through 60 )
9X-RAY DIFFRACTION9chain 'I' and (resid 61 through 151 )
10X-RAY DIFFRACTION10chain 'I' and (resid 152 through 217 )
11X-RAY DIFFRACTION11chain 'H' and (resid 2 through 16 )
12X-RAY DIFFRACTION12chain 'H' and (resid 17 through 39 )
13X-RAY DIFFRACTION13chain 'H' and (resid 40 through 51 )
14X-RAY DIFFRACTION14chain 'H' and (resid 52 through 77 )
15X-RAY DIFFRACTION15chain 'H' and (resid 78 through 92 )
16X-RAY DIFFRACTION16chain 'H' and (resid 93 through 109 )
17X-RAY DIFFRACTION17chain 'H' and (resid 110 through 125 )
18X-RAY DIFFRACTION18chain 'H' and (resid 126 through 151 )
19X-RAY DIFFRACTION19chain 'H' and (resid 152 through 171 )
20X-RAY DIFFRACTION20chain 'H' and (resid 172 through 210 )
21X-RAY DIFFRACTION21chain 'H' and (resid 211 through 220 )
22X-RAY DIFFRACTION22chain 'M' and (resid 1 through 25 )
23X-RAY DIFFRACTION23chain 'M' and (resid 26 through 79 )
24X-RAY DIFFRACTION24chain 'M' and (resid 80 through 106 )
25X-RAY DIFFRACTION25chain 'M' and (resid 107 through 117 )
26X-RAY DIFFRACTION26chain 'M' and (resid 118 through 135 )
27X-RAY DIFFRACTION27chain 'M' and (resid 136 through 167 )
28X-RAY DIFFRACTION28chain 'M' and (resid 168 through 178 )
29X-RAY DIFFRACTION29chain 'M' and (resid 179 through 195 )
30X-RAY DIFFRACTION30chain 'M' and (resid 196 through 216 )
31X-RAY DIFFRACTION31chain 'L' and (resid 1 through 25 )
32X-RAY DIFFRACTION32chain 'L' and (resid 26 through 52 )
33X-RAY DIFFRACTION33chain 'L' and (resid 53 through 65 )
34X-RAY DIFFRACTION34chain 'L' and (resid 66 through 79 )
35X-RAY DIFFRACTION35chain 'L' and (resid 80 through 117 )
36X-RAY DIFFRACTION36chain 'L' and (resid 118 through 141 )
37X-RAY DIFFRACTION37chain 'L' and (resid 142 through 178 )
38X-RAY DIFFRACTION38chain 'L' and (resid 179 through 194 )
39X-RAY DIFFRACTION39chain 'L' and (resid 195 through 218 )

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