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Yorodumi- PDB-6ehd: OmpT (in-vitro folded), an outer membrane protein of Vibrio cholerae -
+Open data
-Basic information
Entry | Database: PDB / ID: 6ehd | ||||||
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Title | OmpT (in-vitro folded), an outer membrane protein of Vibrio cholerae | ||||||
Components | OmpT protein | ||||||
Keywords | MEMBRANE PROTEIN / Outer membrane protein / Porin / OmpF or OmpC ortholog / ion-transport / membrane beta barrel / ion-channel / diffusion porin / diffusion channel / non-specific porin. | ||||||
Function / homology | Gram-negative porin / Porin domain, Gram-negative type / Porin domain superfamily / porin activity / pore complex / monoatomic ion transport / cell outer membrane / OmpT protein Function and homology information | ||||||
Biological species | Vibrio cholerae serotype O1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.66 Å | ||||||
Authors | van den berg, B. / Pathania, M. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Structure / Year: 2018 Title: Unusual Constriction Zones in the Major Porins OmpU and OmpT from Vibrio cholerae. Authors: Pathania, M. / Acosta-Gutierrez, S. / Bhamidimarri, S.P. / Basle, A. / Winterhalter, M. / Ceccarelli, M. / van den Berg, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ehd.cif.gz | 85.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ehd.ent.gz | 63.2 KB | Display | PDB format |
PDBx/mmJSON format | 6ehd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eh/6ehd ftp://data.pdbj.org/pub/pdb/validation_reports/eh/6ehd | HTTPS FTP |
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-Related structure data
Related structure data | 5oykC 6ehbSC 6ehcC 6eheC 6ehfC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35424.594 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (bacteria) Strain: ATCC 39541 / Classical Ogawa 395 / O395 / Gene: ompT, VC0395_A1445 / Plasmid: pET28a / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0H3AME7 | ||||
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#2: Chemical | ChemComp-C8E / ( #3: Chemical | ChemComp-MES / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.46 Å3/Da / Density % sol: 64 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M NaCl, 0.1 M MES, 33% v/v PEG 400, 4% v/v ethylene glycol |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9282 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Aug 10, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9282 Å / Relative weight: 1 |
Reflection | Resolution: 1.66→49.64 Å / Num. obs: 62456 / % possible obs: 99 % / Redundancy: 3.4 % / CC1/2: 0.9 / Rpim(I) all: 0.022 / Net I/σ(I): 14.5 |
Reflection shell | Highest resolution: 1.66 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 1.1 / CC1/2: 0.5 / Rpim(I) all: 0.61 / % possible all: 97.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6EHB Resolution: 1.66→49.64 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.952 / SU B: 2.18 / SU ML: 0.066 / Cross valid method: THROUGHOUT / ESU R: 0.076 / ESU R Free: 0.079 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.331 Å2
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Refinement step | Cycle: 1 / Resolution: 1.66→49.64 Å
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Refine LS restraints |
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