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Yorodumi- PDB-6a30: Crystal Structure of Munc13-1 MUN Domain and Synaptobrevin-2 Juxt... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6a30 | |||||||||||||||
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Title | Crystal Structure of Munc13-1 MUN Domain and Synaptobrevin-2 Juxtamembrane Linker Region | |||||||||||||||
Components |
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Keywords | EXOCYTOSIS / Synaptic exocytosis / Membrane fusion / Munc13 / Munc18 / SNARE / Synaptobrevin | |||||||||||||||
Function / homology | Function and homology information dense core granule priming / neuronal dense core vesicle exocytosis / trans-Golgi Network Vesicle Budding / positive regulation of glutamate receptor signaling pathway / regulation of delayed rectifier potassium channel activity / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / Other interleukin signaling / diacylglycerol binding / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex / synaptobrevin 2-SNAP-25-syntaxin-1a complex ...dense core granule priming / neuronal dense core vesicle exocytosis / trans-Golgi Network Vesicle Budding / positive regulation of glutamate receptor signaling pathway / regulation of delayed rectifier potassium channel activity / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / Other interleukin signaling / diacylglycerol binding / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex / synaptobrevin 2-SNAP-25-syntaxin-1a complex / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex / Lysosome Vesicle Biogenesis / regulation of synaptic vesicle priming / Glutamate Neurotransmitter Release Cycle / Norepinephrine Neurotransmitter Release Cycle / zymogen granule membrane / Acetylcholine Neurotransmitter Release Cycle / Serotonin Neurotransmitter Release Cycle / GABA synthesis, release, reuptake and degradation / regulated exocytosis / Dopamine Neurotransmitter Release Cycle / Golgi Associated Vesicle Biogenesis / presynaptic dense core vesicle exocytosis / storage vacuole / synaptic vesicle docking / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / eosinophil degranulation / vesicle fusion / synaptic vesicle maturation / SNARE complex / Golgi to plasma membrane protein transport / SNAP receptor activity / presynaptic active zone cytoplasmic component / regulation of vesicle-mediated transport / Cargo recognition for clathrin-mediated endocytosis / positive regulation of synaptic plasticity / Clathrin-mediated endocytosis / hormone secretion / calcium-ion regulated exocytosis / innervation / positive regulation of intracellular protein transport / regulation of exocytosis / neurotransmitter secretion / positive regulation of dendrite extension / regulation of short-term neuronal synaptic plasticity / neuron projection terminus / regulation of synaptic vesicle recycling / regulation of amyloid precursor protein catabolic process / syntaxin-1 binding / SNARE complex assembly / positive regulation of neurotransmitter secretion / synaptic vesicle priming / syntaxin binding / clathrin-coated vesicle / Golgi-associated vesicle / spectrin binding / neuromuscular junction development / myosin binding / presynaptic active zone / synaptic vesicle exocytosis / calyx of Held / excitatory synapse / synaptic vesicle endocytosis / response to glucose / amyloid-beta metabolic process / vesicle-mediated transport / SNARE binding / secretory granule / synaptic membrane / synaptic transmission, glutamatergic / long-term synaptic potentiation / establishment of localization in cell / phospholipid binding / neuromuscular junction / cytoplasmic vesicle membrane / trans-Golgi network / terminal bouton / synaptic vesicle membrane / cellular response to insulin stimulus / calcium-dependent protein binding / synaptic vesicle / presynapse / presynaptic membrane / cytoplasmic vesicle / protein-containing complex assembly / vesicle / transmembrane transporter binding / membrane fusion / cell differentiation / molecular adaptor activity / calmodulin binding / axon / protein domain specific binding / synapse / glutamatergic synapse / lipid binding / calcium ion binding / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex Similarity search - Function | |||||||||||||||
Biological species | Rattus norvegicus (Norway rat) | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.793 Å | |||||||||||||||
Authors | Wang, S. / Li, Y. / Gong, J.H. / Ye, S. / Yang, X.F. / Zhang, R.G. / Ma, C. | |||||||||||||||
Funding support | China, 4items
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Citation | Journal: Nat Commun / Year: 2019 Title: Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly. Authors: Wang, S. / Li, Y. / Gong, J.H. / Ye, S. / Yang, X.F. / Zhang, R.G. / Ma, C. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6a30.cif.gz | 235.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6a30.ent.gz | 190.2 KB | Display | PDB format |
PDBx/mmJSON format | 6a30.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a3/6a30 ftp://data.pdbj.org/pub/pdb/validation_reports/a3/6a30 | HTTPS FTP |
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-Related structure data
Related structure data | 4y21S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 61800.781 Da / Num. of mol.: 1 / Fragment: MUN domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Unc13a, Unc13h1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q62768 |
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#2: Protein/peptide | Mass: 926.071 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Rattus norvegicus (Norway rat) / References: UniProt: P63045 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.92 Å3/Da / Density % sol: 79.24 % |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, hanging drop Details: PEG 3350, magnesium nitrate, 2-(N-Morpholino)ethanesulfonic acid (MES) PH range: 5.8 - 6.3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9793 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 18, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 2.79→50 Å / Num. obs: 37919 / % possible obs: 99.9 % / Redundancy: 6.4 % / Rsym value: 0.095 / Net I/σ(I): 24.7 |
Reflection shell | Resolution: 2.8→2.85 Å / Rmerge(I) obs: 0.666 / Num. unique obs: 1840 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4Y21 Resolution: 2.793→42.01 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 27.65
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 209.31 Å2 / Biso mean: 88.51 Å2 / Biso min: 41.23 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.793→42.01 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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