+
Open data
-
Basic information
Entry | Database: PDB / ID: 5z4t | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Complex structure - AxMan113A-M3 | |||||||||
![]() | beta-1,4-mannanas | |||||||||
![]() | ![]() ![]() | |||||||||
Function / homology | ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Jiang, Z.Q. / You, X. / Yang, S.Q. / Huang, P. / Ma, J.W. | |||||||||
![]() | ![]() Title: Structural insights into the catalytic mechanism of a novel glycoside hydrolase family 113 beta-1,4-mannanase fromAmphibacillus xylanus Authors: You, X. / Qin, Z. / Yan, Q. / Yang, S. / Li, Y. / Jiang, Z. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 163.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 125.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 5ylhSC ![]() 5yliC ![]() 5ylkC ![]() 5yllC S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 36223.473 Da / Num. of mol.: 2 / Mutation: E223A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01 Gene: AXY_22370 / Production host: ![]() ![]() ![]() #2: Polysaccharide | #3: Water | ChemComp-HOH / | ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.03 % |
---|---|
Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: HEPES sodium pH 7.5, 2-Propanol, PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225-HS / Detector: CCD / Date: Jun 12, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.68→32.92 Å / Num. obs: 91072 / % possible obs: 93 % / Redundancy: 9.3 % / Rmerge(I) obs: 0.198 / Rsym value: 0.196 / Net I/σ(I): 6.8 |
Reflection shell | Resolution: 1.68→1.74 Å / Num. unique obs: 91702 / CC1/2: 0.976 / % possible all: 93 |
-
Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: 5YLH Resolution: 1.68→32.92 Å / Cross valid method: FREE R-VALUE
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.68→32.92 Å
| ||||||||||||||||
LS refinement shell | Highest resolution: 1.68 Å |