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- PDB-5xtn: Crystal structure of baculoviral sulfhydryl oxidase P33 (C155A, C... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5xtn | ||||||
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Title | Crystal structure of baculoviral sulfhydryl oxidase P33 (C155A, C158A mutant) | ||||||
![]() | FAD-linked sulfhydryl oxidase | ||||||
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Function / homology | FAD-linked sulfhydryl oxidase / Baculovirus P33 / ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kuang, W. / Hu, Z. / Gong, P. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Three Conserved Regions in Baculovirus Sulfhydryl Oxidase P33 Are Critical for Enzymatic Activity and Function Authors: Kuang, W. / Zhang, H. / Wang, M. / Zhou, N.Y. / Deng, F. / Wang, H. / Gong, P. / Hu, Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 216.2 KB | Display | ![]() |
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PDB format | ![]() | 170.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5xkiC ![]() 5xtoC ![]() 5xtpC ![]() 5xtqC ![]() 5xtrC ![]() 3qzyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34462.812 Da / Num. of mol.: 4 / Mutation: C155A, C158A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: P33, ORF92 / Plasmid: pET28 / Production host: ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-FAD / ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.86 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.4 / Details: PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 3, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.54→60 Å / Num. obs: 40775 / % possible obs: 98.7 % / Redundancy: 3.7 % / Biso Wilson estimate: 38.44 Å2 / Rmerge(I) obs: 0.098 / Χ2: 1.032 / Net I/σ(I): 6.7 / Num. measured all: 149523 |
Reflection shell | Resolution: 2.54→2.63 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.401 / Num. unique all: 4172 / Num. unique obs: 4034 / CC1/2: 0.996 / Χ2: 1.021 / % possible all: 98 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3QZY Resolution: 2.54→37.6 Å / SU ML: 0.3 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 33.61
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 99.57 Å2 / Biso mean: 38.63 Å2 / Biso min: 15.38 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.54→37.6 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14
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