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Yorodumi- PDB-5woy: NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra -
+Open data
-Basic information
Entry | Database: PDB / ID: 5woy | ||||||
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Title | NMR solution structure of Enzyme I (nEIt) protein using two 4D-spectra | ||||||
Components | Phosphoenolpyruvate-protein phosphotransferasePhosphoenolpyruvate—protein phosphotransferase | ||||||
Keywords | TRANSFERASE | ||||||
Function / homology | Function and homology information phosphoenolpyruvate-protein phosphotransferase / phosphoenolpyruvate-protein phosphotransferase activity / phosphoenolpyruvate-dependent sugar phosphotransferase system / kinase activity / phosphorylation / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Caldanaerobacter subterraneus subsp. tengcongensis (bacteria) | ||||||
Method | SOLUTION NMR / na | ||||||
Authors | Evangelidis, T. / Nerli, S. / Sgourakis, N.G. / Tripsianes, K. | ||||||
Citation | Journal: Nat Commun / Year: 2018 Title: Automated NMR resonance assignments and structure determination using a minimal set of 4D spectra. Authors: Evangelidis, T. / Nerli, S. / Novacek, J. / Brereton, A.E. / Karplus, P.A. / Dotas, R.R. / Venditti, V. / Sgourakis, N.G. / Tripsianes, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5woy.cif.gz | 750.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5woy.ent.gz | 631.3 KB | Display | PDB format |
PDBx/mmJSON format | 5woy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wo/5woy ftp://data.pdbj.org/pub/pdb/validation_reports/wo/5woy | HTTPS FTP |
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-Related structure data
Related structure data | 5wotC 5woxC 5wozC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 27297.273 Da / Num. of mol.: 1 / Fragment: PEP-utilizers residues 1-248 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (bacteria) Strain: DSM 15242 / JCM 11007 / NBRC 100824 / MB4 / Gene: PtsA, TTE2334 / Production host: Escherichia coli (E. coli) References: UniProt: Q8R7R4, phosphoenolpyruvate-protein phosphotransferase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1.8 mM [U-13C; U-15N] Enzyme I (nEIt) protein, 95% H2O/5% D2O Label: 13C_15N_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 1.8 mM / Component: Enzyme I (nEIt) protein / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 100 mM / Label: conditions_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 850 MHz |
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-Processing
NMR software |
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Refinement | Method: na / Software ordinal: 2 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 |