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- PDB-5wmb: Structure of the 10S (-)-cis-BP-dG modified Rev1 ternary complex ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5wmb | |||||||||
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Title | Structure of the 10S (-)-cis-BP-dG modified Rev1 ternary complex (the BP residue is disordered) | |||||||||
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![]() | transferase/dna / benzo[a]pyrene Rev1 polymerase carcinogen lesion bypass / ![]() | |||||||||
Function / homology | ![]() deoxycytidyl transferase activity / Translesion synthesis by REV1 / Translesion synthesis by POLK / Translesion synthesis by POLI / Termination of translesion DNA synthesis / error-free translesion synthesis / error-prone translesion synthesis / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() synthetic construct (others) | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Rechkoblit, O. / Kolbanovsky, A. / Landes, H. / Geacintov, N.E. / Aggarwal, A.K. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Mechanism of error-free replication across benzo[a]pyrene stereoisomers by Rev1 DNA polymerase. Authors: Rechkoblit, O. / Kolbanovskiy, A. / Landes, H. / Geacintov, N.E. / Aggarwal, A.K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 130.7 KB | Display | ![]() |
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PDB format | ![]() | 94.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5wm1C ![]() 5wm8C ![]() 2aq4S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-DNA chain , 2 types, 2 molecules PT
#1: DNA chain | Mass: 3798.475 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#2: DNA chain | Mass: 5037.278 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Protein , 1 types, 1 molecules A
#3: Protein | ![]() Mass: 49452.965 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: REV1, YOR346W, O6339 / Production host: ![]() ![]() ![]() References: UniProt: P12689, ![]() |
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-Non-polymers , 6 types, 178 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/DCP.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/DCP.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-MG / #5: Chemical | ChemComp-DCP / | ![]() #6: Chemical | ChemComp-GOL / ![]() #7: Chemical | ChemComp-EDO / ![]() #8: Chemical | ChemComp-PEG / | ![]() #9: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.47 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 0.25 M sodium citrate pH 6.0 buffer 15-20% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 24, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.25→65.83 Å / Num. obs: 26824 / % possible obs: 100 % / Redundancy: 7.4 % / Net I/σ(I): 18.6 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2AQ4 Resolution: 2.25→65.83 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.934 / SU B: 6.251 / SU ML: 0.151 / Cross valid method: THROUGHOUT / ESU R: 0.282 / ESU R Free: 0.204 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.837 Å2
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Refinement step | Cycle: 1 / Resolution: 2.25→65.83 Å
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