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Yorodumi- PDB-5w17: Crystal structure of Campylobacter jejuni YCEI protein that cryst... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5w17 | ||||||||||||
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Title | Crystal structure of Campylobacter jejuni YCEI protein that crystallizes with large solvent channels for nanotechnology applications | ||||||||||||
Components | Putative periplasmic proteinPeriplasm | ||||||||||||
Keywords | UNKNOWN FUNCTION / nanomaterial nanoporous | ||||||||||||
Function / homology | Function and homology information Lipid/polyisoprenoid-binding, YceI-like / Lipid/polyisoprenoid-binding, YceI-like / Lipid/polyisoprenoid-binding, YceI-like superfamily / YceI-like domain / YceI-like domain / Lipocalin / Beta Barrel / Mainly Beta Similarity search - Domain/homology | ||||||||||||
Biological species | Campylobacter jejuni subsp. jejuni serotype O:2 (Campylobacter) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.58 Å | ||||||||||||
Authors | Huber, T.R. / Snow, C.D. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: Bioconjug. Chem. / Year: 2018 Title: Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals. Authors: Huber, T.R. / McPherson, E.C. / Keating, C.E. / Snow, C.D. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5w17.cif.gz | 50.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5w17.ent.gz | 33.7 KB | Display | PDB format |
PDBx/mmJSON format | 5w17.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w1/5w17 ftp://data.pdbj.org/pub/pdb/validation_reports/w1/5w17 | HTTPS FTP |
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-Related structure data
Related structure data | 5w2dC 5w2kC 5w2rC 5w2vC 5w2xC 5w2zC 5w30C 5w31C 5w32C 5w37C 5w39C 5w3aC 5w3bC 5w3cC 2fgsS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20199.791 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (Campylobacter) Strain: ATCC 700819 / NCTC 11168 / Gene: Cj0420 / Plasmid: pSB3 / Production host: Escherichia coli (E. coli) / Strain (production host): C41(DE3) / References: UniProt: Q0PB90 | ||
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#2: Chemical | ChemComp-LFA / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Mosaicity: 0.3 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 / Details: 3.2 M Ammonium Sulfate, 0.1 M Bis-Tris |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 4.2.2 / Wavelength: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: RDI CMOS_8M / Detector: CMOS / Date: Apr 4, 2017 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Monochromator: Si (111) double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.58→38.167 Å / Num. obs: 15452 / % possible obs: 99.9 % / Redundancy: 20.3 % / Rpim(I) all: 0.051 / Rrim(I) all: 0.23 / Rsym value: 0.224 / Net I/av σ(I): 2.8 / Net I/σ(I): 10.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2fgs Resolution: 2.58→36.07 Å / Cor.coef. Fo:Fc: 0.95 / Cor.coef. Fo:Fc free: 0.924 / SU B: 11.614 / SU ML: 0.211 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.224 / ESU R Free: 0.204 / Details: U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 159.4 Å2 / Biso mean: 68.195 Å2 / Biso min: 39.17 Å2
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Refinement step | Cycle: final / Resolution: 2.58→36.07 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.58→2.647 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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