+Open data
-Basic information
Entry | Database: PDB / ID: 5u6h | ||||||
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Title | Solution structure of the zinc fingers 1 and 2 of MBNL1 | ||||||
Components | Muscleblind-like protein 1 | ||||||
Keywords | RNA BINDING PROTEIN / Zinc fingers RNA-binding / Structure from MOLMOL | ||||||
Function / homology | Function and homology information regulatory region RNA binding / myoblast differentiation / embryonic limb morphogenesis / regulation of RNA splicing / RNA splicing / mRNA processing / cytoplasmic stress granule / double-stranded RNA binding / nervous system development / in utero embryonic development ...regulatory region RNA binding / myoblast differentiation / embryonic limb morphogenesis / regulation of RNA splicing / RNA splicing / mRNA processing / cytoplasmic stress granule / double-stranded RNA binding / nervous system development / in utero embryonic development / RNA binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Phukan, P.D. / Park, S. / Martinez-Yamout, M.M. / Zeeb, M. / Dyson, H.J. / Wright, P.E. | ||||||
Citation | Journal: Biochemistry / Year: 2017 Title: Structural Basis for Interaction of the Tandem Zinc Finger Domains of Human Muscleblind with Cognate RNA from Human Cardiac Troponin T. Authors: Park, S. / Phukan, P.D. / Zeeb, M. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5u6h.cif.gz | 577.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5u6h.ent.gz | 480.3 KB | Display | PDB format |
PDBx/mmJSON format | 5u6h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u6/5u6h ftp://data.pdbj.org/pub/pdb/validation_reports/u6/5u6h | HTTPS FTP |
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-Related structure data
Related structure data | 5u6lC 5u9bC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10577.180 Da / Num. of mol.: 1 / Fragment: Zinc fingers 1 and 2 (UNP residues 1-92) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MBNL1, EXP, KIAA0428, MBNL / Production host: Escherichia coli (E. coli) / References: UniProt: Q9NR56 |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 0.4 mM [U-98% 13C; U-98% 15N] Zinc fingers 1 and 2 of MBNL1, 90% H2O/10% D2O Label: 13C,15N_sample / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.4 mM / Component: Zinc fingers 1 and 2 of MBNL1 / Isotopic labeling: [U-98% 13C; U-98% 15N] |
Sample conditions | Ionic strength: 50 mM NaCl mM / Label: conditions_1 / pH: 7.0 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |