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- PDB-5t4q: Autoinhibited E. coli ATP synthase state 3 -

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Basic information

Entry
Database: PDB / ID: 5t4q
TitleAutoinhibited E. coli ATP synthase state 3
DescriptorATP synthase gamma chain
ATP synthase epsilon chain
(ATP synthase subunit ...) x 6
KeywordsHYDROLASE / ATP synthase / ATPase / rotary motor / membrane protein
Specimen sourceEscherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
MethodElectron microscopy (8.53 A resolution / Single particle)
AuthorsSobti, M. / Smits, C. / Wong, A.S.W. / Ishmukhametov, R. / Stock, D. / Sandin, S. / Stewart, A.G.
CitationElife, 2016, 5

Elife, 2016, 5 StrPapers
Cryo-EM structures of the autoinhibited E. coli ATP synthase in three rotational states.
Meghna Sobti / Callum Smits / Andrew Sw Wong / Robert Ishmukhametov / Daniela Stock / Sara Sandin / Alastair G Stewart

DateDeposition: Aug 29, 2016 / Release: Jan 4, 2017

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Assembly

Deposited unit
A: ATP synthase subunit alpha
B: ATP synthase subunit alpha
C: ATP synthase subunit alpha
D: ATP synthase subunit beta
E: ATP synthase subunit beta
F: ATP synthase subunit beta
G: ATP synthase gamma chain
H: ATP synthase epsilon chain
I: ATP synthase subunit b
J: ATP synthase subunit b
K: ATP synthase subunit a
L: ATP synthase subunit delta
M: ATP synthase subunit c
N: ATP synthase subunit c
O: ATP synthase subunit c
P: ATP synthase subunit c
Q: ATP synthase subunit c
R: ATP synthase subunit c
S: ATP synthase subunit c
T: ATP synthase subunit c
U: ATP synthase subunit c
V: ATP synthase subunit c
hetero molecules


Theoretical massNumber of molelcules
Total (without water)535,44226
Polyers533,49322
Non-polymers1,9494
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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ATP synthase subunit ... , 6 types, 20 molecules ABCDEFIJKL...

#1: Polypeptide(L)ATP synthase subunit alpha / ATP synthase F1 sector subunit alpha / F-ATPase subunit alpha


Mass: 55138.531 Da / Num. of mol.: 3 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7MGF4, EC: 3.6.3.14
#2: Polypeptide(L)ATP synthase subunit beta / ATP synthase F1 sector subunit beta / F-ATPase subunit beta


Mass: 51664.574 Da / Num. of mol.: 3 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7MGF2, EC: 3.6.3.14
#5: Polypeptide(L)ATP synthase subunit b / ATP synthase F(0) sector subunit b / ATPase subunit I / F-type ATPase subunit b / F-ATPase subunit b


Mass: 17126.691 Da / Num. of mol.: 2 / Source: (gene. exp.) Escherichia coli / References: UniProt: P0ABA2
#6: Polypeptide(L)ATP synthase subunit a / ATP synthase F0 sector subunit a / F-ATPase subunit 6


Mass: 30324.096 Da / Num. of mol.: 1 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7L888
#7: Polypeptide(L)ATP synthase subunit delta / ATP synthase F(1) sector subunit delta / F-type ATPase subunit delta / F-ATPase subunit delta


Mass: 19289.061 Da / Num. of mol.: 1 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7MGF5
#8: Polypeptide(L)
ATP synthase subunit c / ATP synthase F(0) sector subunit c / F-type ATPase subunit c / F-ATPase subunit c / Lipid-binding protein


Mass: 8259.064 Da / Num. of mol.: 10 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7NR39

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Polypeptide(L) , 2 types, 2 molecules GH

#3: Polypeptide(L)ATP synthase gamma chain / ATP synthase F1 sector gamma subunit / F-ATPase gamma subunit


Mass: 31539.285 Da / Num. of mol.: 1 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7MGF3
#4: Polypeptide(L)ATP synthase epsilon chain / ATP synthase F1 sector epsilon subunit / F-ATPase epsilon subunit


Mass: 15087.244 Da / Num. of mol.: 1 / Source: (gene. exp.) Escherichia coli / References: UniProt: B7MGF1

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Non-polymers , 2 types, 4 molecules

#9: ChemicalChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE / ATP (energy-carrying molecule) *YM


Mass: 507.181 Da / Num. of mol.: 3 / Formula: C10H16N5O13P3
#10: ChemicalChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE / ADP *YM


Mass: 427.201 Da / Num. of mol.: 1 / Formula: C10H15N5O10P2

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: ATP synthase / Type: COMPLEX
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD

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Processing

3D reconstructionResolution: 8.53 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 95345

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