+Open data
-Basic information
Entry | Database: PDB / ID: 5mnw | ||||||
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Title | Solution structure of the cinaciguat bound human beta1 H-NOX. | ||||||
Components | Guanylate cyclase soluble subunit beta-1 | ||||||
Keywords | LYASE / soluble guanylate cyclase / H-NOX / cinaciguat | ||||||
Function / homology | Function and homology information cytidylate cyclase activity / guanylate cyclase complex, soluble / trans-synaptic signaling by nitric oxide, modulating synaptic transmission / guanylate cyclase / cGMP biosynthetic process / guanylate cyclase activity / response to oxygen levels / presynaptic active zone cytoplasmic component / Nitric oxide stimulates guanylate cyclase / adenylate cyclase activity ...cytidylate cyclase activity / guanylate cyclase complex, soluble / trans-synaptic signaling by nitric oxide, modulating synaptic transmission / guanylate cyclase / cGMP biosynthetic process / guanylate cyclase activity / response to oxygen levels / presynaptic active zone cytoplasmic component / Nitric oxide stimulates guanylate cyclase / adenylate cyclase activity / blood circulation / nitric oxide-cGMP-mediated signaling / cGMP-mediated signaling / Smooth Muscle Contraction / cellular response to nitric oxide / nitric oxide mediated signal transduction / Hsp90 protein binding / signaling receptor activity / glutamatergic synapse / heme binding / protein-containing complex binding / GTP binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Matzapetakis, M. / Saraiva, I.H. | ||||||
Citation | Journal: To Be Published Title: Solution structure of the cinaciguat bound human beta1 H-NOX. Authors: Matzapetakis, M. / Saraiva, I.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5mnw.cif.gz | 696.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5mnw.ent.gz | 594.7 KB | Display | PDB format |
PDBx/mmJSON format | 5mnw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mn/5mnw ftp://data.pdbj.org/pub/pdb/validation_reports/mn/5mnw | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 22460.486 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GUCY1B3, GUC1B3, GUCSB3, GUCY1B1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q02153, guanylate cyclase |
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#2: Chemical | ChemComp-Z90 / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 1 mM [U-99% 13C; U-99% 15N] beta1 H-NOX, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O Label: H-NOX / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||
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Sample |
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Sample conditions |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 2 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 10 |