+Open data
-Basic information
Entry | Database: PDB / ID: 5mn5 | |||||||||
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Title | S. aureus FtsZ 12-316 T66W GTP Closed form (2TCm) | |||||||||
Components | Cell division protein FtsZ | |||||||||
Keywords | HYDROLASE / bacterial cell division / bacterial cytoskeleton / filamentous / gtpase | |||||||||
Function / homology | Function and homology information chloroplast fission / FtsZ-dependent cytokinesis / division septum assembly / cell division site / protein polymerization / GTPase activity / GTP binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Staphylococcus aureus (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.802 Å | |||||||||
Authors | Wagstaff, J.M. / Tsim, M. / Kureisaite-Ciziene, D. / Lowe, J. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: MBio / Year: 2017 Title: A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments. Authors: Wagstaff, J.M. / Tsim, M. / Oliva, M.A. / Garcia-Sanchez, A. / Kureisaite-Ciziene, D. / Andreu, J.M. / Lowe, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5mn5.cif.gz | 118.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5mn5.ent.gz | 90.7 KB | Display | PDB format |
PDBx/mmJSON format | 5mn5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mn/5mn5 ftp://data.pdbj.org/pub/pdb/validation_reports/mn/5mn5 | HTTPS FTP |
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-Related structure data
Related structure data | 5mn4C 5mn6C 5mn7C 5mn8C 3wglS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 31611.738 Da / Num. of mol.: 2 / Mutation: T66W Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: ftsZ / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P0A031 #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.74 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 10 mg/ml; LiCl 1.136 M, PEG 6,000 31.4 %, 0.1 M MES pH 6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9792 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 4, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 12051 / % possible obs: 98.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.4 % / CC1/2: 0.995 / Rmerge(I) obs: 0.109 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 2.8→2.95 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.554 / Mean I/σ(I) obs: 3 / CC1/2: 0.781 / % possible all: 95.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3WGL Resolution: 2.802→43.245 Å / SU ML: 0.36 / Cross valid method: THROUGHOUT / σ(F): 0.85 / Phase error: 26.77
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.802→43.245 Å
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Refine LS restraints |
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LS refinement shell |
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