+Open data
-Basic information
Entry | Database: PDB / ID: 5lta | ||||||
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Title | Crystal structure of the Prp43-ADP-BeF3-U7-RNA complex | ||||||
Components |
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Keywords | Protein/RNA / RNA HELICASE / DEAH-BOX PROTEIN / DHX15 / Protein-RNA complex | ||||||
Function / homology | Function and homology information RNA helicase activity / nucleic acid binding / hydrolase activity / RNA helicase / ribonucleoprotein complex / ATP binding Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum var. thermophilum DSM 1495 (fungus) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.621 Å | ||||||
Authors | Tauchert, M.J. / Ficner, R. | ||||||
Funding support | Germany, 1items
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Citation | Journal: Elife / Year: 2017 Title: Structural insights into the mechanism of the DEAH-box RNA helicase Prp43. Authors: Tauchert, M.J. / Fourmann, J.B. / Luhrmann, R. / Ficner, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5lta.cif.gz | 302.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5lta.ent.gz | 243.6 KB | Display | PDB format |
PDBx/mmJSON format | 5lta.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lt/5lta ftp://data.pdbj.org/pub/pdb/validation_reports/lt/5lta | HTTPS FTP |
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-Related structure data
Related structure data | 5ltjC 5ltkC 5d0uS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein / RNA chain , 2 types, 2 molecules AE
#1: Protein | Mass: 81641.336 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: THE PROTEIN WAS EXPRESSED WITH 2 ADDITIONAL N-TERMINAL AMINO ACIDS (MA) AND A C-TERMINAL STREP-TAG (WSHPQFEK) Source: (gene. exp.) Chaetomium thermophilum var. thermophilum DSM 1495 (fungus) Gene: CTHT_0005780 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta 2 / References: UniProt: G0RY84, RNA helicase |
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#2: RNA chain | Mass: 4853.695 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Non-polymers , 5 types, 56 molecules
#3: Chemical | ChemComp-ADP / |
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#4: Chemical | ChemComp-MG / |
#5: Chemical | ChemComp-BEF / |
#6: Chemical | ChemComp-CAC / |
#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.55 Å3/Da / Density % sol: 65.31 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 2.5 % (w/v) PGA-LM, 13 % (w/v) PEG 8000 and 100 mM Na Cacodylate pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.82656 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: May 19, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.82656 Å / Relative weight: 1 |
Reflection | Resolution: 2.62→48.56 Å / Num. obs: 36887 / % possible obs: 98.5 % / Redundancy: 5.14 % / Biso Wilson estimate: 65 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.059 / Net I/σ(I): 22.16 |
Reflection shell | Resolution: 2.62→2.7 Å / Redundancy: 5.34 % / Rmerge(I) obs: 0.9 / Mean I/σ(I) obs: 1.85 / CC1/2: 0.652 / % possible all: 86.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5d0u Resolution: 2.621→48.558 Å / SU ML: 0.38 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 23.45
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.621→48.558 Å
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Refine LS restraints |
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LS refinement shell |
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