+Open data
-Basic information
Entry | Database: PDB / ID: 5jol | ||||||
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Title | Calcium-free EF-hand domain of L-plastin | ||||||
Components | Plastin-2 | ||||||
Keywords | METAL BINDING PROTEIN / Calcium-binding / EF-hand / L-plastin | ||||||
Function / homology | Function and homology information actin filament network formation / actin crosslink formation / T cell activation involved in immune response / podosome / positive regulation of podosome assembly / cortical actin cytoskeleton organization / regulation of intracellular protein transport / actin filament bundle / glial cell projection / phagocytic cup ...actin filament network formation / actin crosslink formation / T cell activation involved in immune response / podosome / positive regulation of podosome assembly / cortical actin cytoskeleton organization / regulation of intracellular protein transport / actin filament bundle / glial cell projection / phagocytic cup / actin filament bundle assembly / extracellular matrix disassembly / animal organ regeneration / ruffle / protein kinase A signaling / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / filopodium / actin filament / ruffle membrane / actin filament binding / cell migration / actin cytoskeleton / integrin binding / GTPase binding / cell junction / actin binding / focal adhesion / calcium ion binding / perinuclear region of cytoplasm / extracellular space / extracellular exosome / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Ishida, H. / Jensen, K.V. / Woodman, A.G. / Hyndman, M.E. / Vogel, H.J. | ||||||
Citation | Journal: Sci Rep / Year: 2017 Title: The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling. Authors: Ishida, H. / Jensen, K.V. / Woodman, A.G. / Hyndman, M.E. / Vogel, H.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5jol.cif.gz | 676.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5jol.ent.gz | 569.6 KB | Display | PDB format |
PDBx/mmJSON format | 5jol.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jo/5jol ftp://data.pdbj.org/pub/pdb/validation_reports/jo/5jol | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9274.443 Da / Num. of mol.: 1 / Fragment: EF-hand domain (UNP residues 1-82) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LCP1, PLS2 / Production host: Escherichia coli (E. coli) / References: UniProt: P13796 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: conditions_1 / pH: 6.8 / Pressure: 1 atm / Temperature: 25 C |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 2 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 30 |