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Open data
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Basic information
Entry | Database: PDB / ID: 5j95 | ||||||
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Title | MAP4K4 in complex with inhibitor | ||||||
![]() | Mitogen-activated protein kinase kinase kinase kinase 4 | ||||||
![]() | Transferase/Transferase Inhibitor / ![]() ![]() | ||||||
Function / homology | ![]() positive regulation of ARF protein signal transduction / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, S. | ||||||
![]() | ![]() Title: MAP4K4 in complex with inhibitor Authors: Liu, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 249.5 KB | Display | ![]() |
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PDB format | ![]() | 201.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 35733.059 Da / Num. of mol.: 2 / Fragment: UNP residues 2-313 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: Insect cell expression vector pTIE1 (others) References: UniProt: O95819, ![]() #2: Chemical | ChemComp-6HH / | #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.49 Å3/Da / Density % sol: 50.7 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / Details: 0.1 M Bis-Tris pH=6.5, 0.2 M NaCl, 18% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 23, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→96.4 Å / Num. obs: 25413 / % possible obs: 100 % / Observed criterion σ(F): 0 / Redundancy: 5.1 % / Biso Wilson estimate: 75.38 Å2 / Rmerge(I) obs: 0.103 / Net I/σ(I): 11.7 |
Reflection shell | Resolution: 2.5→2.8 Å / Redundancy: 6.8 % / Rmerge(I) obs: 1.137 / Mean I/σ(I) obs: 1.6 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Displacement parameters | Biso mean: 77.06 Å2
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Refine analyze | Luzzati coordinate error obs: 0.415 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.5→66.66 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.6 Å / Total num. of bins used: 13
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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