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Yorodumi- PDB-5h5j: Complex between ferredoxin and ferredoxin-NADP+ reductase from ma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5h5j | ||||||
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Title | Complex between ferredoxin and ferredoxin-NADP+ reductase from maize root | ||||||
Components |
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Keywords | OXIDOREDUCTASE/ELECTRON TRANSPORT / Electron transfer complex / OXIDOREDUCTASE-ELECTRON TRANSPORT complex | ||||||
Function / homology | Function and homology information etioplast stroma / oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / NADPH binding / photosynthesis / chloroplast / electron transport chain ...etioplast stroma / oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor / ferredoxin-NADP+ reductase / ferredoxin-NADP+ reductase activity / NADPH dehydrogenase activity / chloroplast stroma / NADPH binding / photosynthesis / chloroplast / electron transport chain / 2 iron, 2 sulfur cluster binding / electron transfer activity / nucleotide binding / metal ion binding Similarity search - Function | ||||||
Biological species | Zea mays (maize) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Kurisu, G. / Hase, T. | ||||||
Funding support | Japan, 1items
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Citation | Journal: Photosyn. Res. / Year: 2017 Title: Structural basis for the isotype-specific interactions of ferredoxin and ferredoxin: NADP(+) oxidoreductase: an evolutionary switch between photosynthetic and heterotrophic assimilation Authors: Shinohara, F. / Kurisu, G. / Hanke, G. / Bowsher, C. / Hase, T. / Kimata-Ariga, Y. #1: Journal: Photosyn. Res. / Year: 2004 Title: Fd : FNR Electron Transfer Complexes: Evolutionary Refinement of Structural Interactions Authors: Hanke, G.T. / Kurisu, G. / Kusunoki, M. / Hase, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5h5j.cif.gz | 163.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5h5j.ent.gz | 125.3 KB | Display | PDB format |
PDBx/mmJSON format | 5h5j.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h5/5h5j ftp://data.pdbj.org/pub/pdb/validation_reports/h5/5h5j | HTTPS FTP |
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-Related structure data
Related structure data | 5h57C 5h59SC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 34632.242 Da / Num. of mol.: 2 / Fragment: UNP residues 19-327 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zea mays (maize) / Production host: Escherichia coli (E. coli) References: UniProt: Q41736, UniProt: B4G043*PLUS, ferredoxin-NADP+ reductase #2: Protein | | Mass: 10362.236 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Zea mays (maize) / Gene: FDX3, PFD3 / Production host: Escherichia coli (E. coli) / References: UniProt: P27788 #3: Chemical | #4: Chemical | ChemComp-FES / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.41 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 / Details: 20 % PEG6000, 100 mM Sodium cacodylate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 18, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→50 Å / Num. obs: 91157 / % possible obs: 99.4 % / Redundancy: 3.74 % / Biso Wilson estimate: 19.9 Å2 / Net I/σ(I): 25.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5H59 Resolution: 2.5→19.89 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 4560843.87 / Data cutoff low absF: 0 / Cross valid method: THROUGHOUT / σ(F): 2 / Details: RESOLUTION-DEPENDENT WEIGHTING SCHEME USE
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Solvent computation | Bsol: 35.577 Å2 / ksol: 0.370307 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.2 Å2
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Refine analyze |
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Refinement step | Cycle: 1 / Resolution: 2.5→19.89 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.021 / Total num. of bins used: 6
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Xplor file |
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