NAD-dependentproteindeacetylasesirtuin-2 / Regulatory protein SIR2 homolog 2 / SIR2-like protein 2
Mass: 34416.727 Da / Num. of mol.: 2 / Fragment: UNP residues 56-356 Source method: isolated from a genetically manipulated source Details: Gly1 originates from TEV cleavage site His2/Met3 originate from NdeI restriction site of the vector Source: (gene. exp.) Homo sapiens (human) / Gene: SIRT2, SIR2L, SIR2L2 / Production host: Escherichia coli (E. coli) References: UniProt: Q8IXJ6, Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In linear amides
Resolution: 2.01→46.23 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.935 / SU B: 8.889 / SU ML: 0.132 / Cross valid method: THROUGHOUT / ESU R: 0.198 / ESU R Free: 0.156 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22459
2349
5 %
RANDOM
Rwork
0.21097
-
-
-
obs
0.21166
44359
99.4 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK