+Open data
-Basic information
Entry | Database: PDB / ID: 5aem | |||||||||
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Title | Structure of t131 N-terminal TPR array | |||||||||
Components | TRANSCRIPTION FACTOR TAU 131 KDA SUBUNIT | |||||||||
Keywords | TRANSCRIPTION / RNA POLYMERASE III | |||||||||
Function / homology | Function and homology information 5S class rRNA transcription by RNA polymerase III / transcription factor TFIIIC complex / RNA Polymerase III Transcription Initiation From Type 2 Promoter / transcription initiation at RNA polymerase III promoter / transcription by RNA polymerase III / DNA binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | SACCHAROMYCES CEREVISIAE S288C (yeast) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 3.4 Å | |||||||||
Authors | Taylor, N.M.I. / Muller, C.W. | |||||||||
Citation | Journal: Nat Commun / Year: 2015 Title: Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly. Authors: Male, G. / von Appen, A. / Glatt, S. / Taylor, N.M. / Cristovao, M. / Groetsch, H. / Beck, M. / Muller, C.W. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5aem.cif.gz | 165.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5aem.ent.gz | 134.6 KB | Display | PDB format |
PDBx/mmJSON format | 5aem.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ae/5aem ftp://data.pdbj.org/pub/pdb/validation_reports/ae/5aem | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 52366.895 Da / Num. of mol.: 1 / Fragment: N-TERMINAL TPR ARRAY, RESIDUES 123-566 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE S288C (yeast) / Plasmid: PETM11 / Production host: ESCHERICHIA COLI BL21 (bacteria) / Variant (production host): STAR PRARE / References: UniProt: P33339 |
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Sequence details | ADDITIONAL |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.23 Å3/Da / Density % sol: 76.48 % / Description: NONE |
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Crystal grow | pH: 8.9 / Details: 0.1 M BICINE PH 8.9 0.85 M MGCL2 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-2 / Wavelength: 0.8726 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 4, 2010 / Details: MIRRORS |
Radiation | Monochromator: SI (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 3.4→74.34 Å / Num. obs: 14895 / % possible obs: 99.9 % / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 134.41 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 3.4→3.58 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.59 / Mean I/σ(I) obs: 2 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS Starting model: NONE Resolution: 3.4→74.338 Å / SU ML: 0.35 / σ(F): 1.45 / Phase error: 23.77 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 158.29 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.4→74.338 Å
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Refine LS restraints |
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LS refinement shell |
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