Nitric oxide stimulates guanylate cyclase / negative regulation of hepatic stellate cell contraction / synaptic signaling by nitric oxide / negative regulation of iron ion transmembrane transport / ROS and RNS production in phagocytes / azurophil granule / negative regulation of vasoconstriction / retrograde trans-synaptic signaling by nitric oxide / positive regulation of sodium ion transmembrane transport / response to nitric oxide ...Nitric oxide stimulates guanylate cyclase / negative regulation of hepatic stellate cell contraction / synaptic signaling by nitric oxide / negative regulation of iron ion transmembrane transport / ROS and RNS production in phagocytes / azurophil granule / negative regulation of vasoconstriction / retrograde trans-synaptic signaling by nitric oxide / positive regulation of sodium ion transmembrane transport / response to nitric oxide / Ion homeostasis / nitric oxide metabolic process / postsynaptic specialization, intracellular component / positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / negative regulation of cytosolic calcium ion concentration / peptidyl-cysteine S-nitrosylation / cadmium ion binding / behavioral response to cocaine / positive regulation of the force of heart contraction / negative regulation of potassium ion transport / calyx of Held / negative regulation of calcium ion transport / negative regulation of serotonin uptake / regulation of neurogenesis / sodium channel regulator activity / negative regulation of insulin secretion / response to vitamin E / nitric-oxide synthase (NADPH) / regulation of postsynaptic membrane potential / postsynaptic density, intracellular component / multicellular organismal response to stress / nitric-oxide synthase activity / xenobiotic catabolic process / negative regulation of peptidyl-serine phosphorylation / NADPH binding / arginine catabolic process / striated muscle contraction / regulation of sodium ion transport / nitric oxide mediated signal transduction / T-tubule / sarcoplasmic reticulum membrane / response to organonitrogen compound / cellular response to epinephrine stimulus / nitric oxide biosynthetic process / negative regulation of blood pressure / photoreceptor inner segment / response to nutrient levels / sarcoplasmic reticulum / secretory granule / response to activity / response to hormone / positive regulation of long-term synaptic potentiation / female pregnancy / muscle contraction / establishment of localization in cell / cell periphery / phosphoprotein binding / response to lead ion / response to nicotine / establishment of protein localization / potassium ion transport / response to organic cyclic compound / sarcolemma / cellular response to growth factor stimulus / response to peptide hormone / Z disc / cellular response to mechanical stimulus / response to estrogen / vasodilation / calcium ion transport / calcium-dependent protein binding / FMN binding / positive regulation of peptidyl-serine phosphorylation / NADP binding / flavin adenine dinucleotide binding / ATPase binding / response to heat / scaffold protein binding / nuclear membrane / response to ethanol / mitochondrial outer membrane / negative regulation of neuron apoptotic process / transmembrane transporter binding / response to lipopolysaccharide / dendritic spine / postsynaptic density / cytoskeleton / calmodulin binding / response to hypoxia / membrane raft / negative regulation of cell population proliferation / glutamatergic synapse / synapse / dendrite / heme binding / negative regulation of apoptotic process / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / enzyme binding / positive regulation of transcription by RNA polymerase II Similarity search - Function
NITRICOXIDESYNTHASE, BRAIN / / BNOS / CONSTITUTIVE NOS / NC-NOS / NOS TYPE I / NEURONAL NOS / N-NOS / NNOS / PEPTIDYL-CYSTEINE S- ...BNOS / CONSTITUTIVE NOS / NC-NOS / NOS TYPE I / NEURONAL NOS / N-NOS / NNOS / PEPTIDYL-CYSTEINE S-NITROSYLASE NOS1 / NEURONAL NITRIC OXIDE SYNTHASE
Mass: 48812.527 Da / Num. of mol.: 2 / Fragment: HEME DOMAIN, RESIDUES 297-718 Source method: isolated from a genetically manipulated source Source: (gene. exp.) RATTUS NORVEGICUS (Norway rat) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P29476, nitric-oxide synthase (NADPH)
-
Non-polymers , 6 types, 278 molecules
#2: Chemical
ChemComp-HEM / PROTOPORPHYRINIXCONTAININGFE / HEME / Heme B
Mass: 616.487 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C34H32FeN4O4
Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1 Å / Relative weight: 1
Reflection
Resolution: 1.9→50 Å / Num. obs: 75185 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 4.9 % / Biso Wilson estimate: 29.43 Å2 / Rmerge(I) obs: 0.14 / Net I/σ(I): 6
Reflection shell
Resolution: 1.9→1.96 Å / Redundancy: 4.9 % / Rmerge(I) obs: 1.5 / Mean I/σ(I) obs: 0.8 / % possible all: 100
-
Processing
Software
Name
Version
Classification
PHENIX
(PHENIX.REFINE)
refinement
MOSFLM
datareduction
Aimless
datascaling
REFMAC
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.9→49.276 Å / SU ML: 0.27 / σ(F): 0.01 / Phase error: 29.96 / Stereochemistry target values: ML Details: RESIDUES 339-349 IN CHAIN A AND 339-347 IN CHAIN B ARE DISORDERED.
Rfactor
Num. reflection
% reflection
Rfree
0.2387
7115
5 %
Rwork
0.1964
-
-
obs
0.1985
74815
99.35 %
Solvent computation
Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement step
Cycle: LAST / Resolution: 1.9→49.276 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
6659
0
175
269
7103
Refine LS restraints
Refine-ID
Type
Dev ideal
Number
X-RAY DIFFRACTION
f_bond_d
0.007
7062
X-RAY DIFFRACTION
f_angle_d
1.166
9614
X-RAY DIFFRACTION
f_dihedral_angle_d
15.873
2565
X-RAY DIFFRACTION
f_chiral_restr
0.073
992
X-RAY DIFFRACTION
f_plane_restr
0.005
1213
LS refinement shell
Resolution (Å)
Rfactor Rfree
Num. reflection Rfree
Rfactor Rwork
Num. reflection Rwork
Refine-ID
% reflection obs (%)
1.9-1.9216
0.513
249
0.4998
4514
X-RAY DIFFRACTION
99
1.9216-1.9442
0.3507
234
0.3383
4492
X-RAY DIFFRACTION
99
1.9442-1.9679
0.3541
271
0.3001
4505
X-RAY DIFFRACTION
100
1.9679-1.9928
0.3725
241
0.3096
4528
X-RAY DIFFRACTION
99
1.9928-2.019
0.3399
203
0.2813
4528
X-RAY DIFFRACTION
100
2.019-2.0467
0.3074
243
0.2825
4547
X-RAY DIFFRACTION
99
2.0467-2.0759
0.3553
252
0.2966
4507
X-RAY DIFFRACTION
99
2.0759-2.1069
0.3466
202
0.2754
4543
X-RAY DIFFRACTION
100
2.1069-2.1398
0.3621
196
0.2582
4571
X-RAY DIFFRACTION
100
2.1398-2.1749
0.3383
223
0.2463
4542
X-RAY DIFFRACTION
100
2.1749-2.2124
0.3102
228
0.2655
4515
X-RAY DIFFRACTION
100
2.2124-2.2527
0.4487
239
0.396
4302
X-RAY DIFFRACTION
94
2.2527-2.296
0.3446
285
0.2666
4423
X-RAY DIFFRACTION
99
2.296-2.3429
0.2669
248
0.2182
4555
X-RAY DIFFRACTION
100
2.3429-2.3938
0.2737
226
0.207
4509
X-RAY DIFFRACTION
100
2.3938-2.4495
0.2886
226
0.2125
4617
X-RAY DIFFRACTION
100
2.4495-2.5107
0.2877
222
0.2124
4506
X-RAY DIFFRACTION
100
2.5107-2.5786
0.2769
255
0.2001
4587
X-RAY DIFFRACTION
100
2.5786-2.6545
0.2528
210
0.2043
4531
X-RAY DIFFRACTION
100
2.6545-2.7402
0.2723
243
0.2115
4530
X-RAY DIFFRACTION
100
2.7402-2.8381
0.262
223
0.2012
4577
X-RAY DIFFRACTION
100
2.8381-2.9517
0.2351
243
0.1912
4491
X-RAY DIFFRACTION
100
2.9517-3.086
0.2442
264
0.1873
4554
X-RAY DIFFRACTION
100
3.086-3.2487
0.242
233
0.1903
4575
X-RAY DIFFRACTION
100
3.2487-3.4522
0.2121
219
0.1776
4552
X-RAY DIFFRACTION
100
3.4522-3.7187
0.2091
252
0.1598
4531
X-RAY DIFFRACTION
100
3.7187-4.0927
0.1898
243
0.1476
4486
X-RAY DIFFRACTION
100
4.0927-4.6845
0.1566
248
0.1371
4524
X-RAY DIFFRACTION
99
4.6845-5.9005
0.181
282
0.1461
4444
X-RAY DIFFRACTION
99
5.9005-49.2922
0.1673
212
0.1629
4448
X-RAY DIFFRACTION
97
Refinement TLS params.
Method: refined / Refine-ID: X-RAY DIFFRACTION
ID
L11 (°2)
L12 (°2)
L13 (°2)
L22 (°2)
L23 (°2)
L33 (°2)
S11 (Å °)
S12 (Å °)
S13 (Å °)
S21 (Å °)
S22 (Å °)
S23 (Å °)
S31 (Å °)
S32 (Å °)
S33 (Å °)
T11 (Å2)
T12 (Å2)
T13 (Å2)
T22 (Å2)
T23 (Å2)
T33 (Å2)
Origin x (Å)
Origin y (Å)
Origin z (Å)
1
0.9292
-0.1038
-0.2151
1.3044
-0.143
8.3137
-0.0359
0.0931
-0.0059
0.0478
-0.1197
0.0447
0.1578
-0.4198
0.0695
0.2176
-0.0101
0.011
0.2078
-0.0101
0.2591
11.3082
4.6603
22.5376
2
0.9692
-0.2626
-0.1676
1.27
0.5617
3.6712
0.0048
-0.0201
0.0694
-0.1251
-0.0684
-0.0278
0.1686
0.0839
0.0449
0.1817
0.0162
0.0376
0.2317
0.0223
0.2645
12.3296
4.8174
59.808
Refinement TLS group
ID
Refine-ID
Refine TLS-ID
Selection details
1
X-RAY DIFFRACTION
1
(CHAINAANDRESID299:716)
2
X-RAY DIFFRACTION
2
(CHAINBANDRESID299:718)
+
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