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Open data
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Basic information
Entry | Database: PDB / ID: 4xw4 | ||||||
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Title | X-ray structure of PKAc with AMPPNP, SP20, calcium ions | ||||||
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Function / homology | ![]() spontaneous exocytosis of neurotransmitter / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / negative regulation of meiotic cell cycle / HDL assembly / DARPP-32 events / Rap1 signalling / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gerlits, O. / Tian, J. / Das, A. / Taylor, S. / Langan, P. / Heller, T.W. / Kovalevsky, A. | ||||||
![]() | ![]() Title: Phosphoryl Transfer Reaction Snapshots in Crystals: INSIGHTS INTO THE MECHANISM OF PROTEIN KINASE A CATALYTIC SUBUNIT. Authors: Gerlits, O. / Tian, J. / Das, A. / Langan, P. / Heller, W.T. / Kovalevsky, A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.9 KB | Display | ![]() |
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PDB format | ![]() | 73.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | ![]() Mass: 41566.172 Da / Num. of mol.: 1 / Fragment: UNP residues 15-351 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() | ||||
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#2: Protein/peptide | ![]() Mass: 2199.386 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() ![]() | ||||
#3: Chemical | #4: Chemical | ChemComp-ANP / | #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 52.88 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: 100 mM MES pH 6.5, 5 mM DTT, 15-20% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Nov 13, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.82→40 Å / Num. obs: 36595 / % possible obs: 97.8 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.036 / Net I/σ(I): 33.08 |
Reflection shell | Resolution: 1.82→1.89 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.467 / Mean I/σ(I) obs: 2.2 / % possible all: 94.4 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Refine analyze | Num. disordered residues: 2 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 3297 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.82→20 Å
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Refine LS restraints |
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