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- PDB-4xoi: Structure of hsAnillin bound with RhoA(Q63L) at 2.1 Angstroms res... -

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Basic information

Entry
Database: PDB / ID: 4xoi
TitleStructure of hsAnillin bound with RhoA(Q63L) at 2.1 Angstroms resolution
Components
  • Actin-binding protein anillin
  • Transforming protein RhoA
KeywordsCELL CYCLE / RhoA-anillin complex
Function / homology
Function and homology information


positive regulation of bleb assembly / septin ring organization / actomyosin contractile ring assembly / septin ring assembly / podocyte cell migration / actomyosin contractile ring / aortic valve formation / alpha-beta T cell lineage commitment / mitotic cleavage furrow formation / bone trabecula morphogenesis ...positive regulation of bleb assembly / septin ring organization / actomyosin contractile ring assembly / septin ring assembly / podocyte cell migration / actomyosin contractile ring / aortic valve formation / alpha-beta T cell lineage commitment / mitotic cleavage furrow formation / bone trabecula morphogenesis / positive regulation of lipase activity / endothelial tube lumen extension / bleb / skeletal muscle satellite cell migration / positive regulation of vascular associated smooth muscle contraction / angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure / SLIT2:ROBO1 increases RHOA activity / RHO GTPases Activate Rhotekin and Rhophilins / Roundabout signaling pathway / negative regulation of intracellular steroid hormone receptor signaling pathway / Axonal growth inhibition (RHOA activation) / Axonal growth stimulation / regulation of neural precursor cell proliferation / cleavage furrow formation / regulation of exit from mitosis / regulation of modification of postsynaptic actin cytoskeleton / regulation of osteoblast proliferation / forebrain radial glial cell differentiation / cell junction assembly / apical junction assembly / regulation of systemic arterial blood pressure by endothelin / cellular response to chemokine / negative regulation of cell migration involved in sprouting angiogenesis / beta selection / establishment of epithelial cell apical/basal polarity / regulation of modification of postsynaptic structure / negative regulation of cell size / RHO GTPases Activate ROCKs / negative regulation of oxidative phosphorylation / negative regulation of motor neuron apoptotic process / RHO GTPases activate CIT / PCP/CE pathway / Sema4D induced cell migration and growth-cone collapse / RHO GTPases activate KTN1 / apolipoprotein A-I-mediated signaling pathway / positive regulation of podosome assembly / negative regulation of cell-substrate adhesion / Wnt signaling pathway, planar cell polarity pathway / Sema4D mediated inhibition of cell attachment and migration / ossification involved in bone maturation / positive regulation of alpha-beta T cell differentiation / odontogenesis / motor neuron apoptotic process / PI3K/AKT activation / wound healing, spreading of cells / positive regulation of leukocyte adhesion to vascular endothelial cell / apical junction complex / regulation of focal adhesion assembly / negative chemotaxis / RHOB GTPase cycle / myosin binding / EPHA-mediated growth cone collapse / stress fiber assembly / regulation of neuron projection development / RHOC GTPase cycle / androgen receptor signaling pathway / positive regulation of cytokinesis / cerebral cortex cell migration / ERBB2 Regulates Cell Motility / cellular response to cytokine stimulus / cleavage furrow / semaphorin-plexin signaling pathway / mitotic cytokinesis / Rho protein signal transduction / ficolin-1-rich granule membrane / mitotic spindle assembly / RHOA GTPase cycle / endothelial cell migration / positive regulation of T cell migration / hematopoietic progenitor cell differentiation / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / regulation of microtubule cytoskeleton organization / cytoplasmic microtubule organization / skeletal muscle tissue development / regulation of cell migration / negative regulation of reactive oxygen species biosynthetic process / RHO GTPases activate PKNs / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / EPHB-mediated forward signaling / substantia nigra development / positive regulation of neuron differentiation / substrate adhesion-dependent cell spreading / cell-matrix adhesion / small monomeric GTPase / G protein activity / secretory granule membrane / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / kidney development / RHO GTPases Activate Formins
Similarity search - Function
Anillin homology domain / Anillin, N-terminal domain / Anillin, PH domain / Cell division protein anillin / Anillin N-terminus / Small GTPase Rho / small GTPase Rho family profile. / PH domain / PH domain profile. / Pleckstrin homology domain. ...Anillin homology domain / Anillin, N-terminal domain / Anillin, PH domain / Cell division protein anillin / Anillin N-terminus / Small GTPase Rho / small GTPase Rho family profile. / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / PH-like domain superfamily / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Transforming protein RhoA / Anillin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.092 Å
AuthorsSun, L. / Guan, R. / Lee, I.-J. / Liu, Y. / Chen, M. / Wang, J. / Wu, J. / Chen, Z.
Funding support China, United States, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China31270762 China
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM086546 United States
CitationJournal: Dev.Cell / Year: 2015
Title: Mechanistic insights into the anchorage of the contractile ring by anillin and mid1
Authors: Sun, L. / Guan, R. / Lee, I.J. / Liu, Y. / Chen, M. / Wang, J. / Wu, J.Q. / Chen, Z.
History
DepositionJan 16, 2015Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Jul 15, 2015Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2017Group: Author supporting evidence / Derived calculations ...Author supporting evidence / Derived calculations / Source and taxonomy / Structure summary
Category: entity_src_gen / pdbx_audit_support ...entity_src_gen / pdbx_audit_support / pdbx_struct_oper_list / struct_keywords
Item: _entity_src_gen.pdbx_alt_source_flag / _pdbx_audit_support.funding_organization ..._entity_src_gen.pdbx_alt_source_flag / _pdbx_audit_support.funding_organization / _pdbx_struct_oper_list.symmetry_operation / _struct_keywords.text
Revision 1.2Mar 23, 2022Group: Author supporting evidence / Database references / Derived calculations
Category: database_2 / pdbx_audit_support / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_audit_support.funding_organization / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id
Revision 1.3May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transforming protein RhoA
B: Actin-binding protein anillin
C: Transforming protein RhoA
D: Actin-binding protein anillin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)100,9758
Polymers99,8804
Non-polymers1,0954
Water7,512417
1
A: Transforming protein RhoA
B: Actin-binding protein anillin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,4884
Polymers49,9402
Non-polymers5472
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2390 Å2
ΔGint-29 kcal/mol
Surface area19640 Å2
MethodPISA
2
C: Transforming protein RhoA
D: Actin-binding protein anillin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,4884
Polymers49,9402
Non-polymers5472
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2440 Å2
ΔGint-29 kcal/mol
Surface area19290 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.311, 52.434, 103.101
Angle α, β, γ (deg.)77.87, 74.14, 85.73
Int Tables number1
Space group name H-MP1

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Components

#1: Protein Transforming protein RhoA / / Rho cDNA clone 12 / h12


Mass: 20348.336 Da / Num. of mol.: 2 / Fragment: UNP residues 1-180 / Mutation: Q63L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RHOA, ARH12, ARHA, RHO12 / Production host: Escherichia coli (E. coli) / References: UniProt: P61586
#2: Protein Actin-binding protein anillin


Mass: 29591.893 Da / Num. of mol.: 2 / Fragment: RBD domain, UNP residues 712-981
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ANLN / Production host: Escherichia coli (E. coli) / References: UniProt: Q9NQW6
#3: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate


Mass: 523.180 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 417 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.76 Å3/Da / Density % sol: 55.5 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 12% PEG 3,350, 3% PEG 10000, 0.2 M sodium malonate, 5 mM DTT

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Data collection

DiffractionMean temperature: 103 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.987 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 16, 2012
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.987 Å / Relative weight: 1
ReflectionResolution: 2.092→29.688 Å / Num. obs: 61564 / % possible obs: 98.1 % / Redundancy: 3.3 % / Net I/σ(I): 3

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Processing

Software
NameVersionClassification
PHENIX(phenix.refine: 1.7.1_743)refinement
HKL-2000data reduction
HKL-2000data scaling
SHARPphasing
RefinementResolution: 2.092→29.688 Å / SU ML: 0.5 / Cross valid method: FREE R-VALUE / σ(F): 1.98 / Phase error: 20.81 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2061 2017 3.28 %
Rwork0.1726 --
obs0.1737 61564 97.58 %
Solvent computationShrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 44.837 Å2 / ksol: 0.357 e/Å3
Displacement parameters
Baniso -1Baniso -2Baniso -3
1-2.1418 Å20.7282 Å20.0782 Å2
2--1.0376 Å2-3.2 Å2
3----3.1794 Å2
Refinement stepCycle: LAST / Resolution: 2.092→29.688 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6039 0 66 417 6522
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0076208
X-RAY DIFFRACTIONf_angle_d1.0488406
X-RAY DIFFRACTIONf_dihedral_angle_d17.0652351
X-RAY DIFFRACTIONf_chiral_restr0.072978
X-RAY DIFFRACTIONf_plane_restr0.0041069
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.0919-2.14420.24471260.20413887X-RAY DIFFRACTION90
2.1442-2.20220.21711460.19734273X-RAY DIFFRACTION97
2.2022-2.2670.26361510.19414269X-RAY DIFFRACTION97
2.267-2.34010.27641350.18964255X-RAY DIFFRACTION98
2.3401-2.42370.24741470.194231X-RAY DIFFRACTION98
2.4237-2.52070.27261510.19294275X-RAY DIFFRACTION98
2.5207-2.63540.20911380.18754304X-RAY DIFFRACTION98
2.6354-2.77420.22991440.18734288X-RAY DIFFRACTION98
2.7742-2.94790.25221460.18584266X-RAY DIFFRACTION98
2.9479-3.17520.19871490.18374305X-RAY DIFFRACTION98
3.1752-3.49430.21971420.16654319X-RAY DIFFRACTION99
3.4943-3.99890.16651450.15744287X-RAY DIFFRACTION99
3.9989-5.03420.16951430.14324302X-RAY DIFFRACTION99
5.0342-29.69070.17981540.17034286X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.27551.85631.79514.85944.76128.9148-0.0006-0.1357-0.0321-0.20870.0102-0.06350.5110.18120.01460.510.0915-0.05150.30930.03470.1632-51.25518.233931.4028
22.0074-0.2234-1.07452.01742.73772.0176-0.20020.18970.3196-0.4765-0.12191.2139-0.2145-1.33830.21560.44950.0149-0.02880.5612-0.02130.4515-63.94829.586428.891
32.00299.30391.27249.53611.39264.7319-0.10160.07780.3793-0.30710.11960.49140.1095-0.6425-0.00280.32030.0321-0.02290.40540.01480.2045-56.26118.471719.5384
42.82540.0901-1.23853.856-1.29822.00660.0996-0.35020.60640.7447-0.26910.2061-1.2614-0.74920.15390.62770.11240.01380.4479-0.12990.3608-55.853217.398840.3981
53.70311.2885-0.3933.6767-1.11233.052-0.08590.1421-0.2855-0.12270.0768-0.431.30760.51360.02210.59630.1406-0.02250.3308-0.01450.2106-48.0846.563926.6104
62.00157.07572.76385.75510.95053.37920.13570.5935-0.9203-0.62860.0267-0.30070.78930.198-0.1571.78690.3533-0.11730.1851-0.02210.4486-50.7633-9.11128.335
72.0026-0.51842.67167.5197-1.50773.3591-0.0279-0.0977-0.3650.27480.0936-0.42480.34620.4057-0.09330.86160.4756-0.00850.7537-0.01260.4139-41.2099-0.251929.6215
80.1479-0.2186-0.62361.3875-0.48974.2899-0.065-0.147-0.19670.3247-0.0261-0.19420.81290.26910.09080.84470.1705-0.08340.45950.02490.1892-48.83891.919235.3034
93.9771-0.55545.00160.90291.03659.94910.1209-0.5143-0.6729-0.05790.13770.55311.0102-1.2773-0.32081.3651-0.5438-0.18910.91910.30610.4562-67.1372-8.642731.909
108.7019-4.18283.69422.00341.29832.55340.1034-0.2943-0.1890.4355-0.12480.14810.3454-0.2820.07451.647-0.1475-0.23780.7037-0.04190.4918-65.9932-15.42623.9953
114.6054-0.75984.7912.64133.77852.00010.72151.26040.3689-2.0214-0.6884-0.5013-3.3036-0.80530.03932.0705-0.2052-0.04070.81440.04310.357-58.5131-17.958626.9698
124.79832.45784.0191.64512.3483.5830.22310.292-1.02030.0417-0.1040.1282.3098-0.5334-0.12371.791-0.1218-0.12590.6554-0.0650.3852-57.4831-12.59638.1435
131.3517-0.3806-0.42530.66481.15622.0621-0.0665-0.0268-0.40070.0414-0.0487-0.12180.70680.10760.03891.87510.6357-0.30810.07370.7109-0.3804-48.7127-5.847140.7101
149.2681-7.09746.95757.7027-7.31092.0087-0.5004-0.6329-0.11550.40480.47870.2872-0.0043-0.7504-0.01010.9501-0.33880.06390.76520.00270.3248-64.69681.120635.6586
156.74274.61772.88137.09192.80632.0230.0768-0.866-0.08090.6726-0.15140.02420.4334-0.21080.04840.8304-0.0151-0.02130.4956-0.00930.1509-55.11858.145942.9106
162.00335.51183.61227.53472.79292.00430.28210.0679-0.4886-0.0817-0.28680.01370.1029-0.19070.02590.2274-0.0175-0.08070.28020.05450.4013-72.06914.66670.4915
178.22.74116.18372.60652.01997.86830.2436-0.65840.07870.181-0.15560.23370.2869-0.404-0.10280.1430.02460.02410.20020.03880.2349-56.880313.302411.9835
184.1709-4.19195.21612.0027-1.9878.0726-0.1449-0.66870.53350.7492-0.1059-1.0619-0.56861.05860.2220.3449-0.1253-0.10790.8803-0.12720.4905-35.918119.659123.9558
196.956-0.87423.372.6326-0.67455.9402-0.0273-0.2982-0.10060.00620.0473-0.15370.10930.2269-0.08520.09510.02760.03210.16380.02470.2141-47.545713.01278.4265
202.0125-1.01514.32422.0129-1.97392.015-0.01261.47030.3808-1.4870.3561.15020.0504-0.1984-0.10780.4024-0.0301-0.07210.51010.09710.5254-66.712413.2614-5.3725
214.23251.63823.234.91280.46214.06280.15270.4935-0.9762-0.37690.10790.18230.9773-0.6027-0.41280.4182-0.15850.02760.3949-0.13920.4883-43.0957-2.0735-8.7654
222.01710.02136.7862.66840.232.0133-0.3759-0.32570.73640.24480.003-0.2508-0.63610.08810.28310.1663-0.027-0.01080.3882-0.05390.3839-21.668414.78431.8542
239.47833.11483.97836.3452.4737.7323-0.0075-0.54-0.10880.07040.3054-0.5223-0.1480.4373-0.19670.09990.0220.03710.2868-0.06210.3198-34.235412.5115.3742
241.9998-2.493-1.22465.15821.42199.4086-0.0219-0.4332-0.72180.2083-0.03111.60170.8229-1.77120.04130.4054-0.15930.0790.762-0.07850.776-54.28914.7092-3.1306
259.74992.2659-2.17893.0259-0.00854.0896-0.2075-0.0624-0.51910.1155-0.0536-0.39240.23150.44250.25260.22440.04060.01360.21290.07480.3534-34.00785.88353.7079
266.77881.89132.79744.73010.85265.29570.1186-0.3528-0.8597-0.26340.00760.46851.0307-1.1319-0.13740.352-0.18440.05880.42480.00170.5653-46.5089-0.1967-7.0462
278.75570.92337.79526.84115.48422.0807-0.3708-0.22190.65810.04880.02690.1952-0.4552-0.32160.58160.213-0.15720.10020.3740.00460.2616-39.071714.65941.8138
282.3389-0.6224-0.7982.34550.27753.2283-0.11510.2689-0.0256-0.24150.00380.14150.0566-0.40210.10020.1176-0.074-0.01580.2020.02860.2572-43.227311.1559-5.0648
291.34472.43030.59247.42122.87283.6502-0.27230.2951-0.17-0.15630.2484-0.24080.13050.07030.06190.1938-0.06030.0090.22560.03950.3104-34.141812.8347-9.4786
301.99749.52784.78898.79144.42892.22480.6437-0.5169-0.80570.497-0.3377-1.13410.4047-0.0235-0.29280.5851-0.09430.03010.3634-0.03140.6301-44.3598-7.364-12.0813
316.38252.48652.41192.00255.87916.72120.17380.2926-0.7048-0.0966-0.2640.15260.7959-0.08180.07120.5339-0.04550.06990.4112-0.05330.28-24.2732-23.6664-35.4056
323.5434-1.0273-0.22532.97070.6863.6957-0.23780.052-0.03520.27780.35530.353-0.3184-0.0041-0.12750.3996-0.01190.06250.24630.02360.2496-32.3504-11.4748-26.9731
332.0031-3.64622.49332.002222.0027-0.43770.4194-0.59480.0858-0.8992.22880.4736-1.77541.31550.5882-0.1437-0.06030.6509-0.13410.8197-43.2504-14.8594-26.7714
348.8045-5.0992-2.68053.82210.11173.24580.16880.2592-0.1733-0.21590.11620.492-0.2826-0.263-0.28580.53080.01610.05530.30410.04450.2326-32.7201-14.5331-17.8726
352.71490.62560.33033.5219-4.38492.0042-0.07210.4765-0.4051-0.6880.03650.290.7879-0.86890.0090.6383-0.0555-0.0150.4896-0.15820.3516-32.2265-22.2107-36.5898
362.1010.31163.07772.75253.39522.00910.1590.1863-0.5257-0.5763-0.21250.50590.5214-0.3861-0.00280.5577-0.0596-0.030.3031-0.06850.2944-28.6228-22.5547-35.2132
378.2009-1.6048-0.30052.0065-4.44392.002-0.2148-0.8660.88951.4569-0.0526-0.2369-0.89630.18760.27010.6596-0.0086-0.04080.3278-0.07790.3625-23.5055-10.698-15.6234
380.93350.53550.49730.8398-0.671.9036-0.13920.12490.025-0.23130.195-0.0465-0.3480.2197-0.0670.441-0.06240.040.316-0.01190.2086-24.0448-10.0724-28.6298
392.0036-9.0346-2.07716.7947-1.71759.8770.0123-0.63911.68890.1635-0.009-0.9527-1.29310.5138-0.0530.6696-0.15460.02430.3849-0.04410.4304-22.33792.4111-26.3515
404.6859-1.515-0.86663.07840.75132.94670.09810.36250.4761-0.3027-0.0828-0.3742-0.55930.2801-0.02160.4601-0.11720.00850.35640.04810.2391-24.4706-5.5431-30.6131
412.00777.77746.67646.06725.17662.00430.00090.1921.1071-0.2503-0.31780.8668-0.9089-0.65030.29560.75410.11350.02760.38150.05030.4839-40.265610.4948-23.5277
425.69570.5642-0.43289.02199.33139.82810.03980.05330.5606-0.49230.18070.1346-0.84370.0124-0.15470.89620.00010.05270.35510.04260.3942-32.297211.4452-26.3756
432.004-6.12720.26576.2599-2.62365.82270.34340.48440.7962-0.4949-0.2632-0.3913-0.60140.0639-0.09971.0412-0.1990.09470.54020.10340.3081-26.03884.0691-38.0667
445.08310.8491-2.26964.1946-0.42022.7154-0.09260.82490.187-0.7811-0.02420.3144-0.7612-0.09230.09740.6214-0.0609-0.05650.41770.04110.1781-32.4592-6.8866-36.862
454.3433-0.88462.78958.27081.62032.00780.14690.6098-0.0479-0.640.0876-0.20790.13510.435-0.20140.786-0.07920.04860.4835-0.06630.1758-25.7955-15.6817-42.7094
462.0037-0.7099-1.42478.34436.06822.00870.35910.25221.0271-0.157-0.26760.8995-0.7836-0.8602-0.05010.26480.01270.09930.33690.08550.5635-48.2434-11.79270.4546
478.653-3.6798-8.16243.30082.45188.3510.2620.3587-0.0613-0.2953-0.09320.3117-0.3047-0.3307-0.180.1477-0.0345-0.00890.17090.01210.1956-33.3907-19.9132-10.0322
482.00182.8304-0.66149.89920.37947.7320.2440.4532-0.2588-0.4239-0.0435-0.89140.4691.1675-0.27820.37290.09760.0350.4913-0.05760.3417-15.2642-26.9069-20.4163
494.02890.3447-1.5631.5527-0.16594.37660.0975-0.0265-0.017-0.1172-0.0057-0.10460.01040.4508-0.12640.1213-0.0050.00060.2298-0.01460.2329-21.8848-20.1084-8.4666
502.0121-1.2027-4.43167.3906-0.5862.01120.0874-0.8563-0.08750.61320.2320.4803-0.0492-0.0434-0.33760.1560.0309-0.02360.39410.08160.3603-42.1291-19.57295.7479
511.98651.5939-0.74921.2551-0.58760.27130.1384-0.71261.07450.8471-0.28770.89550.154-0.4820.04650.77860.24590.22251.039-0.09721.0044-37.3529-4.012217.5207
526.4267-4.1172-1.70319.79951.62475.15370.0552-0.27150.85230.06090.1835-0.3926-0.57680.0467-0.23310.18710.004-0.01560.13410.00860.3267-15.6625-5.91068.2594
539.935-1.1917-3.92284.14111.60562.012-0.23430.3707-0.7999-0.20960.2623-0.3540.41740.4909-0.02110.1470.01610.01730.3282-0.04730.42931.4192-21.06570.1645
540.8971-0.7233-2.32724.50812.35736.34890.0470.60620.1297-0.1880.08580.0975-0.083-0.283-0.17970.13170.0113-0.07450.2893-0.01290.3952-17.293-16.5822-0.4223
552.0051-2.23113.20293.16630.56234.7031-0.23770.09340.2173-0.1537-0.0494-0.9309-0.32750.82730.29360.1862-0.0281-0.02440.32140.08860.5253-11.0409-12.0422-1.5947
563.9791-1.0245-0.45044.93010.60334.49070.00380.04420.24140.275-0.01420.3923-0.3085-0.3931-0.01860.13930.0259-0.01050.20960.04180.3274-21.4927-10.74676.8477
570.41640.7077-0.35921.2115-0.59210.31290.02130.1816-0.9777-0.33310.0036-0.13460.37070.0099-0.00720.22970.0445-0.01510.2739-0.12060.3706-19.9511-28.4035-9.4788
581.7762-0.1932-0.40685.40573.11425.5316-0.038-0.22910.02080.5663-0.18280.1690.0273-0.15290.11560.1530.02680.00440.18980.00150.29-17.3458-13.601213.7166
594.2188-5.6801-1.7928.1733.62533.4815-0.1637-0.2772-0.03170.25770.1694-0.18420.14480.1035-0.03210.17660.03410.00990.21750.02970.2876-10.3591-22.432410.4379
609.3032-6.6701-8.74182.02387.81698.4629-0.0924-0.34371.54290.17040.0357-0.4364-0.9039-0.3555-0.00930.29820.1135-0.03730.2691-0.03860.6155-17.3672-2.337512.7407
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(CHAIN A AND RESID 4:18)A4 - 18
2X-RAY DIFFRACTION2(CHAIN A AND RESID 19:32)A19 - 32
3X-RAY DIFFRACTION3(CHAIN A AND RESID 33:40)A33 - 40
4X-RAY DIFFRACTION4(CHAIN A AND RESID 41:54)A41 - 54
5X-RAY DIFFRACTION5(CHAIN A AND RESID 55:86)A55 - 86
6X-RAY DIFFRACTION6(CHAIN A AND RESID 87:97)A87 - 97
7X-RAY DIFFRACTION7(CHAIN A AND RESID 98:105)A98 - 105
8X-RAY DIFFRACTION8(CHAIN A AND RESID 106:119)A106 - 119
9X-RAY DIFFRACTION9(CHAIN A AND RESID 120:124)A120 - 124
10X-RAY DIFFRACTION10(CHAIN A AND RESID 125:133)A125 - 133
11X-RAY DIFFRACTION11(CHAIN A AND RESID 134:138)A134 - 138
12X-RAY DIFFRACTION12(CHAIN A AND RESID 139:143)A139 - 143
13X-RAY DIFFRACTION13(CHAIN A AND RESID 144:156)A144 - 156
14X-RAY DIFFRACTION14(CHAIN A AND RESID 157:165)A157 - 165
15X-RAY DIFFRACTION15(CHAIN A AND RESID 166:180)A166 - 180
16X-RAY DIFFRACTION16(CHAIN B AND RESID 4:8)B4 - 8
17X-RAY DIFFRACTION17(CHAIN B AND RESID 9:33)B9 - 33
18X-RAY DIFFRACTION18(CHAIN B AND RESID 34:40)B34 - 40
19X-RAY DIFFRACTION19(CHAIN B AND RESID 41:63)B41 - 63
20X-RAY DIFFRACTION20(CHAIN B AND RESID 64:72)B64 - 72
21X-RAY DIFFRACTION21(CHAIN B AND RESID 88:101)B88 - 101
22X-RAY DIFFRACTION22(CHAIN B AND RESID 102:114)B102 - 114
23X-RAY DIFFRACTION23(CHAIN B AND RESID 115:125)B115 - 125
24X-RAY DIFFRACTION24(CHAIN B AND RESID 126:131)B126 - 131
25X-RAY DIFFRACTION25(CHAIN B AND RESID 132:147)B132 - 147
26X-RAY DIFFRACTION26(CHAIN B AND RESID 148:166)B148 - 166
27X-RAY DIFFRACTION27(CHAIN B AND RESID 167:176)B167 - 176
28X-RAY DIFFRACTION28(CHAIN B AND RESID 222:244)B222 - 244
29X-RAY DIFFRACTION29(CHAIN B AND RESID 245:263)B245 - 263
30X-RAY DIFFRACTION30(CHAIN B AND RESID 264:269)B264 - 269
31X-RAY DIFFRACTION31(CHAIN C AND RESID 4:8)C4 - 8
32X-RAY DIFFRACTION32(CHAIN C AND RESID 9:26)C9 - 26
33X-RAY DIFFRACTION33(CHAIN C AND RESID 27:32)C27 - 32
34X-RAY DIFFRACTION34(CHAIN C AND RESID 33:40)C33 - 40
35X-RAY DIFFRACTION35(CHAIN C AND RESID 41:49)C41 - 49
36X-RAY DIFFRACTION36(CHAIN C AND RESID 50:59)C50 - 59
37X-RAY DIFFRACTION37(CHAIN C AND RESID 60:70)C60 - 70
38X-RAY DIFFRACTION38(CHAIN C AND RESID 71:89)C71 - 89
39X-RAY DIFFRACTION39(CHAIN C AND RESID 90:97)C90 - 97
40X-RAY DIFFRACTION40(CHAIN C AND RESID 98:124)C98 - 124
41X-RAY DIFFRACTION41(CHAIN C AND RESID 125:133)C125 - 133
42X-RAY DIFFRACTION42(CHAIN C AND RESID 134:139)C134 - 139
43X-RAY DIFFRACTION43(CHAIN C AND RESID 140:149)C140 - 149
44X-RAY DIFFRACTION44(CHAIN C AND RESID 150:171)C150 - 171
45X-RAY DIFFRACTION45(CHAIN C AND RESID 172:180)C172 - 180
46X-RAY DIFFRACTION46(CHAIN D AND RESID 4:11)D4 - 11
47X-RAY DIFFRACTION47(CHAIN D AND RESID 12:30)D12 - 30
48X-RAY DIFFRACTION48(CHAIN D AND RESID 31:37)D31 - 37
49X-RAY DIFFRACTION49(CHAIN D AND RESID 38:61)D38 - 61
50X-RAY DIFFRACTION50(CHAIN D AND RESID 62:71)D62 - 71
51X-RAY DIFFRACTION51(CHAIN D AND RESID 72:91)D72 - 91
52X-RAY DIFFRACTION52(CHAIN D AND RESID 92:101)D92 - 101
53X-RAY DIFFRACTION53(CHAIN D AND RESID 102:114)D102 - 114
54X-RAY DIFFRACTION54(CHAIN D AND RESID 115:131)D115 - 131
55X-RAY DIFFRACTION55(CHAIN D AND RESID 132:147)D132 - 147
56X-RAY DIFFRACTION56(CHAIN D AND RESID 148:175)D148 - 175
57X-RAY DIFFRACTION57(CHAIN D AND RESID 176:227)D176 - 227
58X-RAY DIFFRACTION58(CHAIN D AND RESID 228:248)D228 - 248
59X-RAY DIFFRACTION59(CHAIN D AND RESID 249:261)D249 - 261
60X-RAY DIFFRACTION60(CHAIN D AND RESID 262:268)D262 - 268

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