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Yorodumi- PDB-4wvw: Chicken Galectin-8 N-terminal domain complexed with 3'-sialyl-lactose -
+Open data
-Basic information
Entry | Database: PDB / ID: 4wvw | |||||||||
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Title | Chicken Galectin-8 N-terminal domain complexed with 3'-sialyl-lactose | |||||||||
Components | Galectin | |||||||||
Keywords | SUGAR BINDING PROTEIN / Lectin / carbohydrate recognition domain | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Gallus gallus (chicken) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.47 Å | |||||||||
Authors | Ruiz, F.M. / Romero, A. | |||||||||
Citation | Journal: Chemistry / Year: 2015 Title: Combining Crystallography and Hydrogen-Deuterium Exchange to Study Galectin-Ligand Complexes. Authors: Ruiz, F.M. / Gilles, U. / Lindner, I. / Andre, S. / Romero, A. / Reusch, D. / Gabius, H.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4wvw.cif.gz | 82.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4wvw.ent.gz | 59 KB | Display | PDB format |
PDBx/mmJSON format | 4wvw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wv/4wvw ftp://data.pdbj.org/pub/pdb/validation_reports/wv/4wvw | HTTPS FTP |
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-Related structure data
Related structure data | 4wvvC 2yv8S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 16524.283 Da / Num. of mol.: 1 / Fragment: N-terminal domain, UNP residues 9-152 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Plasmid: pqe60 / Production host: Escherichia coli (E. coli) / References: UniProt: Q1W2P6 |
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#2: Polysaccharide | N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose / 3'-sialyl-alpha-lactose |
#3: Chemical | ChemComp-PEG / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.6 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 30% w/v PEG 4000, 100 Mm Tris-HCl pH 8.5, 200 mM Sodium Acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.98011 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 24, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98011 Å / Relative weight: 1 |
Reflection | Resolution: 1.47→49.75 Å / Num. all: 33211 / Num. obs: 33211 / % possible obs: 100 % / Redundancy: 16.5 % / Rmerge(I) obs: 0.13 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 1.47→1.5 Å / Redundancy: 14.9 % / Rmerge(I) obs: 1.807 / Mean I/σ(I) obs: 2.2 / Num. measured obs: 23984 / Num. unique all: 1610 / % possible all: 100 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2YV8 Resolution: 1.47→47.48 Å / SU ML: 0.14 / Cross valid method: FREE R-VALUE / σ(F): 1.39 / Phase error: 18.07 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 68.05 Å2 / Biso mean: 21.5999 Å2 / Biso min: 8.15 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.47→47.48 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 22 / % reflection obs: 100 %
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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