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- PDB-4v8m: High-resolution cryo-electron microscopy structure of the Trypano... -

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Entry
Database: PDB / ID: 4v8m
TitleHigh-resolution cryo-electron microscopy structure of the Trypanosoma brucei ribosome
Components
  • (40S RIBOSOMAL PROTEIN ...) x 25
  • (60S RIBOSOMAL PROTEIN ...) x 35
  • (RIBOSOMAL PROTEIN ...) x 9
  • 18S RRNA OF THE SMALL RIBOSOMAL SUBUNIT
  • 40S RIBOSOMAL PROTEINS S11, PUTATIVERibosome
  • 5.8S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT
  • 5S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT
  • 60S RIBOSOMAL SUBUNIT PROTEIN L31, PUTATIVE
  • ALPHA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA
  • BETA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA
  • E-SITE TRNA
  • GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN
  • PROBABLE 60S RIBOSOMAL PROTEIN L14
  • RIBOSOMAL PROTEIN
  • SHORT RRNA-I OF THE LARGE RIBOSOMAL SUBUNIT
  • SHORT RRNA-II OF THE LARGE RIBOSOMAL SUBUNIT
  • SHORT RRNA-III OF THE LARGE RIBOSOMAL SUBUNIT
  • SHORT RRNA-IV OF THE LARGE RIBOSOMAL SUBUNIT
  • UBIQUITIN-60S RIBOSOMAL PROTEIN L40
  • UBIQUITIN/RIBOSOMAL PROTEIN S27A, PUTATIVE
KeywordsRIBOSOME / EUKARYOTIC / KINETOPLASTIDS / EXPANSION SEGMENTS
Function / homology
Function and homology information


organellar small ribosomal subunit / organellar large ribosomal subunit / ciliary transition zone / nuclear lumen / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / phosphate ion binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...organellar small ribosomal subunit / organellar large ribosomal subunit / ciliary transition zone / nuclear lumen / mitochondrial large ribosomal subunit / positive regulation of translational fidelity / phosphate ion binding / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / ribosome assembly / small-subunit processome / protein kinase C binding / regulation of cell growth / modification-dependent protein catabolic process / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / protein tag activity / ribosomal small subunit assembly / rRNA processing / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / large ribosomal subunit / ribosome biogenesis / regulation of cell population proliferation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / translation / ribonucleoprotein complex / mRNA binding / ubiquitin protein ligase binding / nucleolus / enzyme binding / RNA binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein S12e / : / S27a-like superfamily / Ribosomal protein S25 ...: / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein S12e / : / S27a-like superfamily / Ribosomal protein S25 / Ribosomal protein S26e signature. / Ribosomal protein S2, eukaryotic / Ribosomal protein S30 / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L29e / Ribosomal protein S3, eukaryotic/archaeal / S25 ribosomal protein / Ribosomal protein L10e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein L10e / Ribosomal protein S19A/S15e / Ribosomal L29e protein family / Ribosomal protein S30 / Ribosomal protein S12e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal protein S27a / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / 40S ribosomal protein S11, N-terminal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein L1, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal S17 / Ribosomal protein L44e / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal protein L27e / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein L27e superfamily / Ribosomal protein S21e / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein S19e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal L38e protein family / Ribosomal L22e protein family / 40S Ribosomal protein S10 / 60S ribosomal protein L35 / Ribosomal protein L1 / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S27 / Ribosomal protein S28e conserved site / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S28e / Ribosomal protein L30e, conserved site / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal_S17 N-terminal / Plectin/S10, N-terminal / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein L44 / Ribosomal protein L34Ae / Ribosomal protein S7e / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Plectin/S10 domain / Ribosomal protein S8e / Ribosomal L27e protein family / Ribosomal protein S4, KOW domain / Ribosomal Protein L6, KOW domain / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein L30/YlxQ / Ribosomal protein L13e / Ribosomal protein L13e / RS4NT (NUC023) domain / Ribosomal protein L34e / Ribosomal protein L1 signature. / Ribosomal protein L37ae
Similarity search - Domain/homology
: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / 60S ribosomal protein L44 / Ubiquitin-ribosomal protein eL40 fusion protein / Large ribosomal subunit protein eL18 / Probable 60S ribosomal protein L14 ...: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / 60S ribosomal protein L44 / Ubiquitin-ribosomal protein eL40 fusion protein / Large ribosomal subunit protein eL18 / Probable 60S ribosomal protein L14 / 60S ribosomal protein L27, putative / 60S ribosomal protein L18a / 40S ribosomal protein S17, putative / Guanine nucleotide-binding protein subunit beta-like protein / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL22 / 60S ribosomal protein L29 / Large ribosomal subunit protein eL34 / 40S ribosomal protein S27, putative / Ribosomal protein / 40S ribosomal protein S26 / 40S ribosomal protein S21, putative / 40S ribosomal protein S5, putative / Large ribosomal subunit protein eL28 / 60S ribosomal protein L17, putative / 40S ribosomal protein S12 / 40S ribosomal protein S4 / Ribosomal protein L37 / 40S ribosomal protein S2, putative / 40S ribosomal protein S3, putative / 60S ribosomal protein L6, putative / 40S ribosomal protein S24 / 60S ribosomal protein L37a, putative / 60S ribosomal protein L24, putative / 40S ribosomal protein S10, putative / 40S ribosomal protein S18, putative / 60S ribosomal protein L30 / Small ribosomal subunit protein eS1 / 60S ribosomal protein L9, putative / 40S ribosomal protein S23, putative / 40S ribosomal proteins S11, putative / 60S ribosomal protein L32, putative / 40S ribosomal protein S6 / Ribosomal protein L36, putative / Ribosomal protein L15 / 60S ribosomal protein L5, putative / 60S ribosomal protein L26, putative / 60S ribosomal subunit protein L31, putative / 60S ribosomal protein L27a / 60S ribosomal protein L23, putative / 60S ribosomal protein L10, putative / 60S ribosomal protein L11, putative / 40S ribosomal protein S7 / 60S ribosomal protein L38, putative / 60S ribosomal protein L35, putative / 60S ribosomal protein L21E, putative / 40S ribosomal protein S14 / 40S ribosomal protein S33, putative / 60S ribosomal protein L39, putative / 60S ribosomal protein L7, putative / 60S ribosomal protein L13 / 60S ribosomal protein L7a / 40S ribosomal protein S9, putative / 40S ribosomal protein S16, putative / 60S ribosomal protein L2, putative / Ubiquitin-ribosomal protein eL40 fusion protein / 40S ribosomal protein S15, putative / 40S ribosomal protein S25 / 60S ribosomal protein L12, putative / 40S ribosomal protein S8 / 60S ribosomal protein L23a / 60S ribosomal protein L19, putative / 40S ribosomal protein S15a, putative / Ribosomal protein L3, mitochondrial, putative / 60S ribosomal protein L4 / Ribosomal protein S19, putative / 60S ribosomal protein L35A, putative / 40S ribosomal protein S30 / 60S ribosomal protein L13a, putative / 40S ribosomal protein S13, putative
Similarity search - Component
Biological speciesTRYPANOSOMA BRUCEI BRUCEI (eukaryote)
TRYPANOSOMA BRUCEI (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 5.57 Å
AuthorsHashem, Y. / des Georges, A. / Fu, J. / Buss, S.N. / Jossinet, F. / Jobe, A. / Zhang, Q. / Liao, H.Y. / Grassucci, R.A. / Bajaj, C. ...Hashem, Y. / des Georges, A. / Fu, J. / Buss, S.N. / Jossinet, F. / Jobe, A. / Zhang, Q. / Liao, H.Y. / Grassucci, R.A. / Bajaj, C. / Westhof, E. / Madison-Antenucci, S. / Frank, J.
CitationJournal: Nature / Year: 2013
Title: High-resolution cryo-electron microscopy structure of the Trypanosoma brucei ribosome.
Authors: Yaser Hashem / Amedee des Georges / Jie Fu / Sarah N Buss / Fabrice Jossinet / Amy Jobe / Qin Zhang / Hstau Y Liao / Robert A Grassucci / Chandrajit Bajaj / Eric Westhof / Susan Madison- ...Authors: Yaser Hashem / Amedee des Georges / Jie Fu / Sarah N Buss / Fabrice Jossinet / Amy Jobe / Qin Zhang / Hstau Y Liao / Robert A Grassucci / Chandrajit Bajaj / Eric Westhof / Susan Madison-Antenucci / Joachim Frank /
Abstract: Ribosomes, the protein factories of living cells, translate genetic information carried by messenger RNAs into proteins, and are thus involved in virtually all aspects of cellular development and ...Ribosomes, the protein factories of living cells, translate genetic information carried by messenger RNAs into proteins, and are thus involved in virtually all aspects of cellular development and maintenance. The few available structures of the eukaryotic ribosome reveal that it is more complex than its prokaryotic counterpart, owing mainly to the presence of eukaryote-specific ribosomal proteins and additional ribosomal RNA insertions, called expansion segments. The structures also differ among species, partly in the size and arrangement of these expansion segments. Such differences are extreme in kinetoplastids, unicellular eukaryotic parasites often infectious to humans. Here we present a high-resolution cryo-electron microscopy structure of the ribosome of Trypanosoma brucei, the parasite that is transmitted by the tsetse fly and that causes African sleeping sickness. The atomic model reveals the unique features of this ribosome, characterized mainly by the presence of unusually large expansion segments and ribosomal-protein extensions leading to the formation of four additional inter-subunit bridges. We also find additional rRNA insertions, including one large rRNA domain that is not found in other eukaryotes. Furthermore, the structure reveals the five cleavage sites of the kinetoplastid large ribosomal subunit (LSU) rRNA chain, which is known to be cleaved uniquely into six pieces, and suggests that the cleavage is important for the maintenance of the T. brucei ribosome in the observed structure. We discuss several possible implications of the large rRNA expansion segments for the translation-regulation process. The structure could serve as a basis for future experiments aimed at understanding the functional importance of these kinetoplastid-specific ribosomal features in protein-translation regulation, an essential step towards finding effective and safe kinetoplastid-specific drugs.
History
DepositionDec 9, 2012Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 9, 2014Provider: repository / Type: Initial release
SupersessionDec 10, 2014ID: 3ZEQ, 3ZEX, 3ZEY, 3ZF7
Revision 1.1Dec 10, 2014Group: Other
Revision 1.2Apr 22, 2015Group: Other
Revision 2.0Aug 2, 2017Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations
Category: atom_site / em_software ...atom_site / em_software / pdbx_validate_close_contact / struct_conn
Item: _atom_site.Cartn_x / _atom_site.Cartn_y ..._atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _em_software.fitting_id / _em_software.image_processing_id / _pdbx_validate_close_contact.auth_atom_id_2
Revision 3.0Jul 31, 2019Group: Advisory / Data collection ...Advisory / Data collection / Database references / Derived calculations / Non-polymer description / Other / Structure summary
Category: chem_comp / database_PDB_caveat ...chem_comp / database_PDB_caveat / pdbx_database_status / pdbx_struct_sheet_hbond / pdbx_validate_close_contact / struct_conf / struct_conn / struct_conn_type / struct_ref_seq_dif / struct_sheet / struct_sheet_order / struct_sheet_range
Item: _chem_comp.formula / _chem_comp.formula_weight ..._chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _pdbx_database_status.process_site / _struct_ref_seq_dif.db_mon_id
Revision 3.1Aug 21, 2019Group: Data collection / Database references / Category: struct_ref_seq_dif / Item: _struct_ref_seq_dif.details
Revision 3.2Oct 23, 2019Group: Data collection / Database references / Category: struct_ref_seq_dif / Item: _struct_ref_seq_dif.details
Revision 3.3Dec 11, 2019Group: Other / Category: atom_sites
Item: _atom_sites.fract_transf_matrix[1][1] / _atom_sites.fract_transf_matrix[2][2] / _atom_sites.fract_transf_matrix[3][3]
Remark 700 SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "5B" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "5B" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "EA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "SA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.

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Assembly

Deposited unit
A0: 40S RIBOSOMAL PROTEIN S3A, PUTATIVE
A1: 40S RIBOSOMAL PROTEIN S4, PUTATIVE
A2: 40S RIBOSOMAL PROTEIN S5, PUTATIVE
A3: 40S RIBOSOMAL PROTEIN S6
A4: RIBOSOMAL PROTEIN S7, PUTATIVE
A5: 40S RIBOSOMAL PROTEIN S8
A6: 40S RIBOSOMAL PROTEIN S9, PUTATIVE
A7: GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN
A8: RIBOSOMAL PROTEIN S29, PUTATIVE
A9: UBIQUITIN/RIBOSOMAL PROTEIN S27A, PUTATIVE
AC: 40S RIBOSOMAL PROTEIN SA, PUTATIVE
AD: 40S RIBOSOMAL PROTEIN S10, PUTATIVE
AE: 40S RIBOSOMAL PROTEINS S11, PUTATIVE
AF: 40S RIBOSOMAL PROTEIN S12
AG: 40S RIBOSOMAL PROTEIN S13, PUTATIVE
AH: 40S RIBOSOMAL PROTEIN S14
AI: 40S RIBOSOMAL PROTEIN S15, PUTATIVE
AJ: 40S RIBOSOMAL PROTEIN S15A, PUTATIVE
AK: 40S RIBOSOMAL PROTEIN S16, PUTATIVE
AL: 40S RIBOSOMAL PROTEIN S17, PUTATIVE
AM: 40S RIBOSOMAL PROTEIN S18, PUTATIVE
AO: RIBOSOMAL PROTEIN S19, PUTATIVE
AP: 40S RIBOSOMAL PROTEIN S2, PUTATIVE
AQ: RIBOSOMAL PROTEIN S20, PUTATIVE
AR: 40S RIBOSOMAL PROTEIN S21, PUTATIVE
AS: 40S RIBOSOMAL PROTEIN S23, PUTATIVE
AT: 40S RIBOSOMAL PROTEIN S24
AU: 40S RIBOSOMAL PROTEIN S25, PUTATIVE
AV: RIBOSOMAL PROTEIN S26, PUTATIVE
AW: 40S RIBOSOMAL PROTEIN S27, PUTATIVE
AX: 40S RIBOSOMAL PROTEIN S3, PUTATIVE
AY: 40S RIBOSOMAL PROTEIN S30, PUTATIVE
AZ: 40S RIBOSOMAL PROTEIN S33, PUTATIVE
BA: ALPHA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA
BB: BETA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA
BC: 5.8S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT
BD: 5S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT
BE: SHORT RRNA-I OF THE LARGE RIBOSOMAL SUBUNIT
BF: SHORT RRNA-II OF THE LARGE RIBOSOMAL SUBUNIT
BG: SHORT RRNA-III OF THE LARGE RIBOSOMAL SUBUNIT
BH: SHORT RRNA-IV OF THE LARGE RIBOSOMAL SUBUNIT
BI: 60S RIBOSOMAL PROTEIN L18
BJ: RIBOSOMAL PROTEIN
BK: 60S RIBOSOMAL PROTEIN L10, PUTATIVE
BL: 60S RIBOSOMAL PROTEIN L11, PUTATIVE
BM: 60S RIBOSOMAL PROTEIN L12, PUTATIVE
BN: 60S RIBOSOMAL PROTEIN L13
BO: 60S RIBOSOMAL PROTEIN L13A, PUTATIVE
BP: PROBABLE 60S RIBOSOMAL PROTEIN L14
BQ: RIBOSOMAL PROTEIN L15
BR: 60S RIBOSOMAL PROTEIN L17, PUTATIVE
BS: 60S RIBOSOMAL PROTEIN L18A
BT: 60S RIBOSOMAL PROTEIN L19, PUTATIVE
BU: 60S RIBOSOMAL PROTEIN L21E, PUTATIVE
BV: 60S RIBOSOMAL PROTEIN L22, PUTATIVE
BW: 60S RIBOSOMAL PROTEIN L23, PUTATIVE
BX: 60S RIBOSOMAL PROTEIN L23A
BY: 60S RIBOSOMAL PROTEIN L24, PUTATIVE
BZ: 60S RIBOSOMAL PROTEIN L26, PUTATIVE
Ba: 60S RIBOSOMAL PROTEIN L27, PUTATIVE
Bb: 60S RIBOSOMAL PROTEIN L27A
Bc: 60S RIBOSOMAL PROTEIN L28, PUTATIVE
Bd: 60S RIBOSOMAL PROTEIN L29, PUTATIVE
Be: 60S RIBOSOMAL PROTEIN L2, PUTATIVE
Bf: RIBOSOMAL PROTEIN L3, MITOCHONDRIAL, PUTATIVE
Bg: 60S RIBOSOMAL PROTEIN L30
Bh: 60S RIBOSOMAL SUBUNIT PROTEIN L31, PUTATIVE
Bi: 60S RIBOSOMAL PROTEIN L32, PUTATIVE
Bj: 60S RIBOSOMAL PROTEIN L34, PUTATIVE
Bk: 60S RIBOSOMAL PROTEIN L35, PUTATIVE
Bl: 60S RIBOSOMAL PROTEIN L35A, PUTATIVE
Bm: RIBOSOMAL PROTEIN L36, PUTATIVE
Bn: RIBOSOMAL PROTEIN L37
Bo: 60S RIBOSOMAL PROTEIN L37A, PUTATIVE
Bp: 60S RIBOSOMAL PROTEIN L38, PUTATIVE
Bq: 60S RIBOSOMAL PROTEIN L39, PUTATIVE
Br: 60S RIBOSOMAL PROTEIN L4
Bs: UBIQUITIN-60S RIBOSOMAL PROTEIN L40
Bt: 60S RIBOSOMAL PROTEIN L44
Bu: 60S RIBOSOMAL PROTEIN L5, PUTATIVE
Bv: 60S RIBOSOMAL PROTEIN L6, PUTATIVE
Bw: 60S RIBOSOMAL PROTEIN L7, PUTATIVE
Bx: 60S RIBOSOMAL PROTEIN L7A, PUTATIVE
By: 60S RIBOSOMAL PROTEIN L9, PUTATIVE
AA: 18S RRNA OF THE SMALL RIBOSOMAL SUBUNIT
AB: E-SITE TRNA


Theoretical massNumber of molelcules
Total (without water)3,603,26986
Polymers3,603,26986
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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40S RIBOSOMAL PROTEIN ... , 25 types, 25 molecules A0A1A2A3A5A6ACADAFAGAHAIAJAKALAMAPARASATAUAWAXAYAZ

#1: Protein 40S RIBOSOMAL PROTEIN S3A, PUTATIVE / Ribosome / RIBOSOMAL PROTEINS S1E


Mass: 29477.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38BK6
#2: Protein 40S RIBOSOMAL PROTEIN S4, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S4E


Mass: 30704.182 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q386F2
#3: Protein 40S RIBOSOMAL PROTEIN S5, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S7


Mass: 21424.857 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q385D9
#4: Protein 40S RIBOSOMAL PROTEIN S6 / / RIBOSOMAL PROTEIN S6E


Mass: 28507.367 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CL9
#6: Protein 40S RIBOSOMAL PROTEIN S8 / / RIBOSOMAL PROTEIN S8E


Mass: 25001.439 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57YL6
#7: Protein 40S RIBOSOMAL PROTEIN S9, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S4


Mass: 22098.736 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57VP3
#11: Protein 40S RIBOSOMAL PROTEIN SA, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S2


Mass: 31395.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383I8
#12: Protein 40S RIBOSOMAL PROTEIN S10, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S10E


Mass: 19322.801 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38B66
#14: Protein 40S RIBOSOMAL PROTEIN S12 / / RIBOSOMAL PROTEIN S12E


Mass: 15889.246 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q385Y8
#15: Protein 40S RIBOSOMAL PROTEIN S13, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S15


Mass: 17347.598 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q586C2
#16: Protein 40S RIBOSOMAL PROTEIN S14 / / RIBOSOMAL PROTEIN S11


Mass: 15540.926 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q4FKG9
#17: Protein 40S RIBOSOMAL PROTEIN S15, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS19


Mass: 17512.377 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57XN7
#18: Protein 40S RIBOSOMAL PROTEIN S15A, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS8


Mass: 14669.270 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q580K6
#19: Protein 40S RIBOSOMAL PROTEIN S16, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS9


Mass: 17031.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57VV0
#20: Protein 40S RIBOSOMAL PROTEIN S17, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS17E


Mass: 16259.235 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q382Z8
#21: Protein 40S RIBOSOMAL PROTEIN S18, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS13


Mass: 17548.488 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38B69
#23: Protein 40S RIBOSOMAL PROTEIN S2, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS5


Mass: 28688.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q388D3
#25: Protein 40S RIBOSOMAL PROTEIN S21, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS21E


Mass: 21156.686 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q385B8
#26: Protein 40S RIBOSOMAL PROTEIN S23, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS12


Mass: 15963.674 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CD1
#27: Protein 40S RIBOSOMAL PROTEIN S24 / / RIBOSOMAL PROTEIN RPS24E


Mass: 15722.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38AM9
#28: Protein 40S RIBOSOMAL PROTEIN S25, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS25E


Mass: 12589.099 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57XX0
#30: Protein 40S RIBOSOMAL PROTEIN S27, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS27E


Mass: 9648.325 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383V7
#31: Protein 40S RIBOSOMAL PROTEIN S3, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS3


Mass: 24259.408 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q389I7
#32: Protein 40S RIBOSOMAL PROTEIN S30, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS30E


Mass: 7454.948 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q584U5
#33: Protein 40S RIBOSOMAL PROTEIN S33, PUTATIVE / Ribosome


Mass: 11186.885 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57U30

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RIBOSOMAL PROTEIN ... , 9 types, 9 molecules A4A8AOAQAVBQBfBmBn

#5: Protein RIBOSOMAL PROTEIN S7, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S7E


Mass: 23889.201 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38FD9
#9: Protein RIBOSOMAL PROTEIN S29, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS14


Mass: 6679.840 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1
#22: Protein RIBOSOMAL PROTEIN S19, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS19E


Mass: 18891.506 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q583K7
#24: Protein RIBOSOMAL PROTEIN S20, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS10


Mass: 13248.478 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1
#29: Protein RIBOSOMAL PROTEIN S26, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN RPS26E


Mass: 12837.207 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q384J9
#50: Protein RIBOSOMAL PROTEIN L15 /


Mass: 26461.006 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CY7
#65: Protein RIBOSOMAL PROTEIN L3, MITOCHONDRIAL, PUTATIVE / Ribosome


Mass: 48802.996 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q581Q1
#72: Protein RIBOSOMAL PROTEIN L36, PUTATIVE / Ribosome


Mass: 12491.966 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CY6
#73: Protein RIBOSOMAL PROTEIN L37 /


Mass: 10128.931 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q386L1

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Protein , 7 types, 7 molecules A7A9AEBJBPBhBs

#8: Protein GUANINE NUCLEOTIDE-BINDING PROTEIN BETA SUBUNIT-LIKE PROTEIN / RIBOSOMAL PROTEIN TRACK1


Mass: 34727.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383E1
#10: Protein UBIQUITIN/RIBOSOMAL PROTEIN S27A, PUTATIVE / RIBOSOMAL PROTEIN RPS31E


Mass: 16936.711 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57WI3
#13: Protein 40S RIBOSOMAL PROTEINS S11, PUTATIVE / Ribosome / RIBOSOMAL PROTEIN S17


Mass: 20132.459 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CI4
#43: Protein RIBOSOMAL PROTEIN /


Mass: 24640.195 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383V8
#49: Protein PROBABLE 60S RIBOSOMAL PROTEIN L14


Mass: 21742.795 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: P55842
#67: Protein 60S RIBOSOMAL SUBUNIT PROTEIN L31, PUTATIVE /


Mass: 21752.922 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38DH8
#78: Protein UBIQUITIN-60S RIBOSOMAL PROTEIN L40


Mass: 14685.345 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: P21899

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RNA chain , 10 types, 10 molecules BABBBCBDBEBFBGBHAAAB

#34: RNA chain ALPHA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA


Mass: 595796.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote) / References: GenBank: X14553
#35: RNA chain BETA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT 28S RRNA


Mass: 471450.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote) / References: GenBank: X14553
#36: RNA chain 5.8S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 54224.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#37: RNA chain 5S RRNA CHAIN OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 38316.672 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#38: RNA chain SHORT RRNA-I OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 67234.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#39: RNA chain SHORT RRNA-II OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 23079.566 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#40: RNA chain SHORT RRNA-III OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 58889.910 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#41: RNA chain SHORT RRNA-IV OF THE LARGE RIBOSOMAL SUBUNIT


Mass: 43388.613 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)
#85: RNA chain 18S RRNA OF THE SMALL RIBOSOMAL SUBUNIT


Mass: 724377.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote) / References: GenBank: M12676
#86: RNA chain E-SITE TRNA


Mass: 23545.971 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI (eukaryote)

+
60S RIBOSOMAL PROTEIN ... , 35 types, 35 molecules BIBKBLBMBNBOBRBSBTBUBVBWBXBYBZBaBbBcBdBeBgBiBjBkBlBoBpBqBrBt...

#42: Protein 60S RIBOSOMAL PROTEIN L18 /


Mass: 21929.154 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: P50885
#44: Protein 60S RIBOSOMAL PROTEIN L10, PUTATIVE / Ribosome


Mass: 24785.303 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38EC5
#45: Protein 60S RIBOSOMAL PROTEIN L11, PUTATIVE / Ribosome


Mass: 22420.361 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38EI9
#46: Protein 60S RIBOSOMAL PROTEIN L12, PUTATIVE / Ribosome


Mass: 17594.693 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57YK4
#47: Protein 60S RIBOSOMAL PROTEIN L13 /


Mass: 25491.955 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57V54
#48: Protein 60S RIBOSOMAL PROTEIN L13A, PUTATIVE / Ribosome


Mass: 25775.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q585J5
#51: Protein 60S RIBOSOMAL PROTEIN L17, PUTATIVE / Ribosome


Mass: 19147.668 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q385T6
#52: Protein 60S RIBOSOMAL PROTEIN L18A /


Mass: 20950.473 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q382C4
#53: Protein 60S RIBOSOMAL PROTEIN L19, PUTATIVE / Ribosome


Mass: 29494.129 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57ZI7
#54: Protein 60S RIBOSOMAL PROTEIN L21E, PUTATIVE / Ribosome


Mass: 18043.301 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q4FKD4
#55: Protein 60S RIBOSOMAL PROTEIN L22, PUTATIVE / Ribosome


Mass: 15288.859 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383R3
#56: Protein 60S RIBOSOMAL PROTEIN L23, PUTATIVE / Ribosome


Mass: 14989.697 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38DP2
#57: Protein 60S RIBOSOMAL PROTEIN L23A /


Mass: 18211.551 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57ZH2
#58: Protein 60S RIBOSOMAL PROTEIN L24, PUTATIVE / Ribosome


Mass: 14633.324 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38B56
#59: Protein 60S RIBOSOMAL PROTEIN L26, PUTATIVE / Ribosome


Mass: 16497.545 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38D25
#60: Protein 60S RIBOSOMAL PROTEIN L27, PUTATIVE / Ribosome


Mass: 15618.485 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q381J6
#61: Protein 60S RIBOSOMAL PROTEIN L27A /


Mass: 16307.155 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38DN9
#62: Protein 60S RIBOSOMAL PROTEIN L28, PUTATIVE / Ribosome


Mass: 16901.014 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q385E9
#63: Protein 60S RIBOSOMAL PROTEIN L29, PUTATIVE / Ribosome


Mass: 8244.586 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383S6
#64: Protein 60S RIBOSOMAL PROTEIN L2, PUTATIVE / Ribosome


Mass: 28372.105 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57W56
#66: Protein 60S RIBOSOMAL PROTEIN L30 /


Mass: 11531.440 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38BI9
#68: Protein 60S RIBOSOMAL PROTEIN L32, PUTATIVE / Ribosome


Mass: 15432.183 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CL1
#69: Protein 60S RIBOSOMAL PROTEIN L34, PUTATIVE / Ribosome


Mass: 19396.330 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q383V6
#70: Protein 60S RIBOSOMAL PROTEIN L35, PUTATIVE / Ribosome


Mass: 15187.259 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38FW8
#71: Protein 60S RIBOSOMAL PROTEIN L35A, PUTATIVE / Ribosome


Mass: 17061.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q584G9
#74: Protein 60S RIBOSOMAL PROTEIN L37A, PUTATIVE / Ribosome


Mass: 10482.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38AW9
#75: Protein 60S RIBOSOMAL PROTEIN L38, PUTATIVE / Ribosome


Mass: 9527.440 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38FV6
#76: Protein 60S RIBOSOMAL PROTEIN L39, PUTATIVE / Ribosome


Mass: 6567.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57UJ8
#77: Protein 60S RIBOSOMAL PROTEIN L4 /


Mass: 41941.230 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q582N2
#79: Protein 60S RIBOSOMAL PROTEIN L44 /


Mass: 12488.846 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: D7SG81
#80: Protein 60S RIBOSOMAL PROTEIN L5, PUTATIVE / Ribosome


Mass: 34710.223 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CY8
#81: Protein 60S RIBOSOMAL PROTEIN L6, PUTATIVE / Ribosome


Mass: 21232.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q389K1
#82: Protein 60S RIBOSOMAL PROTEIN L7, PUTATIVE / Ribosome


Mass: 29416.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q57V35
#83: Protein 60S RIBOSOMAL PROTEIN L7A, PUTATIVE / Ribosome


Mass: 30945.590 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / References: UniProt: Q57VM1
#84: Protein 60S RIBOSOMAL PROTEIN L9, PUTATIVE / Ribosome


Mass: 21897.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) TRYPANOSOMA BRUCEI BRUCEI (eukaryote) / Strain: 927/4 GUTAT10.1 / References: UniProt: Q38CD0

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Details

Sequence detailsCHAINS 8 AND Q ARE MODELLED USING T. CRUZI SEQUENCE UNP ACCESSIONS Q4CPB5 AND Q4D6H7 RESPECTIVELY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: TRYPANOSOMA BRUCEI 80S RIBOSOME / Type: RIBOSOME / Details: MICROGRAPHS SELECTED VISUALLY AFTER SCANNING
Buffer solutionName: 20 MM HEPES, 10MM MGCL2, 500 MM KCL AND 5 MM BETA-ME / pH: 7.2
Details: 20 MM HEPES, 10MM MGCL2, 500 MM KCL AND 5 MM BETA-ME
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: CARBON
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Details: LIQUID ETHAN

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Electron microscopy imaging

Experimental equipment
Model: Tecnai F30 / Image courtesy: FEI Company
MicroscopyModel: FEI TECNAI F30 / Date: Jan 1, 2011
Details: MICROGRAPHS SCANNED WITH NIKON SUPER COOLSCAN 9000ED
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 59000 X / Nominal defocus max: 4000 nm / Nominal defocus min: 1500 nm / Cs: 2 mm
Specimen holderTemperature: 93.15 K
Image recordingElectron dose: 25 e/Å2 / Film or detector model: KODAK SO-163 FILM
Image scansNum. digital images: 1102
Radiation wavelengthRelative weight: 1

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Processing

EM software
IDNameCategory
1UCSF Chimeramodel fitting
2SPIDER3D reconstruction
CTF correctionDetails: PHASE-FLIPPING
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionMethod: REFERENCE BASED RECONSTRUCTION METHOD / Resolution: 5.57 Å / Num. of particles: 164000 / Nominal pixel size: 1.09 Å
Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2239 (DEPOSITION ID: 11259). THE STRUCTURE OF THE 80S FROM YEAST (3U5B AND OTHERS) AS WELL AS THE STRUCTURE OF THE 60S FROM TETRAHYMENA ...Details: SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-2239 (DEPOSITION ID: 11259). THE STRUCTURE OF THE 80S FROM YEAST (3U5B AND OTHERS) AS WELL AS THE STRUCTURE OF THE 60S FROM TETRAHYMENA THERMOPHILA (4A17 AND OTHERS) WERE USED AS STARTING MODEL FOR THE 60S SUBUNIT MODEL. THE 40S FROM TETRAHYMENA THERMOPHILA (2XZM AND 2XZN) AS WELL AS THE 80S FROM YEAST WERE USED AS STARTING MODEL FOR THE 40S SUBUNIT MODEL. THE 80S MODEL OF TRITICUM AESTIVUM (3IZR AND OTHERS) WAS USED TO FIT MISSING PROTEINS FORM THE TWO X-RAY STRUCTURES.
Symmetry type: POINT
Atomic model buildingProtocol: RIGID BODY FIT / Space: REAL / Details: REFINEMENT PROTOCOL--RIGID BODY
Atomic model building
IDPDB-ID 3D fitting-ID
13U5B

3u5b
PDB Unreleased entry

1
23U5C

3u5c
PDB Unreleased entry

1
33U5D

3u5d
PDB Unreleased entry

1
43U5E

3u5e
PDB Unreleased entry

1
53U5F

3u5f
PDB Unreleased entry

1
63U5G

3u5g
PDB Unreleased entry

1
73U5H

3u5h
PDB Unreleased entry

1
83U5I

3u5i
PDB Unreleased entry

1
94A18

4a18
PDB Unreleased entry

1
104A1E

4a1e
PDB Unreleased entry

1
114A17

4a17
PDB Unreleased entry

1
124A19

4a19
PDB Unreleased entry

1
134A1A

4a1a
PDB Unreleased entry

1
144A1B

4a1b
PDB Unreleased entry

1
154A1C

4a1c
PDB Unreleased entry

1
164A1D

4a1d
PDB Unreleased entry

1
172XZM

2xzm
PDB Unreleased entry

1
182XZN

2xzn
PDB Unreleased entry

1
193IZR

3izr
PDB Unreleased entry

1
203IZ6

3iz6
PDB Unreleased entry

1
213IZ7

3iz7
PDB Unreleased entry

1
223IZ9

3iz9
PDB Unreleased entry

1
RefinementHighest resolution: 5.57 Å
Refinement stepCycle: LAST / Highest resolution: 5.57 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms38755 0 0 0 38755

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