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Yorodumi- PDB-4u2n: Crystal structure of a complex of the Miz1- and Nac1 POZ domains. -
+Open data
-Basic information
Entry | Database: PDB / ID: 4u2n | ||||||
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Title | Crystal structure of a complex of the Miz1- and Nac1 POZ domains. | ||||||
Components | Zinc finger and BTB domain-containing protein 17,Nucleus accumbens-associated protein 1 | ||||||
Keywords | TRANSCRIPTION / POZ domain / BTB domain | ||||||
Function / homology | Function and homology information ectoderm development / XBP1(S) activates chaperone genes / regulation of immune system process / gastrulation with mouth forming second / G1 to G0 transition / negative regulation of cell cycle / core promoter sequence-specific DNA binding / regulation of cytokine production / protein-DNA complex / transcription coactivator binding ...ectoderm development / XBP1(S) activates chaperone genes / regulation of immune system process / gastrulation with mouth forming second / G1 to G0 transition / negative regulation of cell cycle / core promoter sequence-specific DNA binding / regulation of cytokine production / protein-DNA complex / transcription coactivator binding / DNA-binding transcription repressor activity, RNA polymerase II-specific / histone deacetylase binding / cell junction / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / positive regulation of cell population proliferation / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / metal ion binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.3 Å | ||||||
Authors | Stead, M.A. / Wright, S.C. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2014 Title: Structures of heterodimeric POZ domains of Miz1/BCL6 and Miz1/NAC1. Authors: Stead, M.A. / Wright, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4u2n.cif.gz | 102.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4u2n.ent.gz | 77.6 KB | Display | PDB format |
PDBx/mmJSON format | 4u2n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u2/4u2n ftp://data.pdbj.org/pub/pdb/validation_reports/u2/4u2n | HTTPS FTP |
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-Related structure data
Related structure data | 4u2mSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Details | The biological unit is a dimer. There are two biological units in the asymmetric unit. |
-Components
#1: Protein | Mass: 27860.744 Da / Num. of mol.: 2 / Fragment: UNP residues 2-115,UNP residues 2-125 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ZBTB17, MIZ1, ZNF151, ZNF60, NACC1, BTBD14B, NAC1 / Plasmid: pGEX-6P-1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q13105, UniProt: Q96RE7 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.28 Å3/Da / Density % sol: 62.55 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 5.6 / Details: 0.2 % PEG 4000, 0.1 M sodium citrate pH 5.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.97949 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 25, 2014 |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50.93 Å / Num. obs: 33315 / % possible obs: 99.7 % / Redundancy: 4 % / Rmerge(I) obs: 0.03 / Net I/σ(I): 24 |
Reflection shell | Resolution: 2.3→2.36 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.663 / Mean I/σ(I) obs: 2.4 / % possible all: 99.7 |
-Phasing
Phasing | Method: molecular replacement | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4U2M Resolution: 2.3→50.93 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.908 / WRfactor Rfree: 0.2814 / WRfactor Rwork: 0.2344 / FOM work R set: 0.74 / SU R Cruickshank DPI: 0.2555 / SU Rfree: 0.2267 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.255 / ESU R Free: 0.227 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 133.07 Å2 / Biso mean: 64.693 Å2 / Biso min: 20 Å2
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Refinement step | Cycle: final / Resolution: 2.3→50.93 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.36 Å / Total num. of bins used: 20
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