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Open data
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Basic information
Entry | Database: PDB / ID: 4u16 | ||||||
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Title | M3-mT4L receptor bound to NMS | ||||||
![]() | Muscarinic acetylcholine receptor M3,Lysozyme,Muscarinic acetylcholine receptor M3 | ||||||
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Function / homology | ![]() negative regulation of heart rate by acetylcholine / G protein-coupled acetylcholine receptor binding / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Thorsen, T.S. / Matt, R. / Weis, W.I. / Kobilka, B. | ||||||
![]() | ![]() Title: Modified T4 Lysozyme Fusion Proteins Facilitate G Protein-Coupled Receptor Crystallogenesis. Authors: Thorsen, T.S. / Matt, R. / Weis, W.I. / Kobilka, B.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 315 KB | Display | ![]() |
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PDB format | ![]() | 254.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4u14C ![]() 4u15C ![]() 4dajS ![]() 4lzmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Details | biological unit is the same as asym. |
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Components
#1: Protein | Mass: 47858.023 Da / Num. of mol.: 2 Fragment: UNP P08483 residues 57-259, 482-563, UNP D9IEF7 residues 61-161 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() Gene: Chrm3, Chrm-3, e, T4Tp126 / Production host: ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ![]() Sequence details | The fusion protein is a chimeric of M3 and RB69 lysozyme. The fusion protein is made of M 3 ( ...The fusion protein is a chimeric of M3 and RB69 lysozyme. The fusion protein is made of M 3 ( residues 57-259) - Lysozyme (residues 1000-1117)- M3 (residues 482-563). | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.24 Å3/Da / Density % sol: 70.96 % |
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Crystal grow![]() | Temperature: 293 K / Method: lipidic cubic phase Details: The best crystallization condition was 100 mM Tris pH 7.5, 44% PEG 300 and 400 mM ammonium tartrate. |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 16, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.7→33.7 Å / Num. obs: 14787 / % possible obs: 93.7 % / Redundancy: 2.6 % / Biso Wilson estimate: 119.14 Å2 / Rmerge(I) obs: 0.219 / Net I/σ(I): 8.9 |
Reflection shell | Resolution: 3.7→3.83 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.884 / Mean I/σ(I) obs: 2.2 / % possible all: 89.1 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4DAJ, 4LZM Resolution: 3.7→33.67 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 34.09 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.7→33.67 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -37.3762 Å / Origin y: -1.074 Å / Origin z: 5.547 Å
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Refinement TLS group | Selection details: all |