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Open data
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Basic information
Entry | Database: PDB / ID: 4rz9 | ||||||
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Title | Pre-mRNA-splicing factor 38A AS 1-179 | ||||||
![]() | Pre-mRNA-splicing factor 38A | ||||||
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Function / homology | ![]() U2-type precatalytic spliceosome / precatalytic spliceosome / mRNA Splicing - Major Pathway / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schuetze, T. / Weber, G. / Wahl, M.C. | ||||||
![]() | ![]() Title: The N-terminal domain of the unusual SR protein hPrp38 is an interaction hub in the spliceosome Authors: Schuetze, T. / Apelt, L. / Ulrich, A. / Weber, G. / Bartlick, N. / Stelzl, U. / Wahl, M.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 144.5 KB | Display | ![]() |
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PDB format | ![]() | 120 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21161.561 Da / Num. of mol.: 1 / Fragment: UNP residues 27-179 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.64 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.1 M MES pH 6.5, and 15% (w/v) PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Sep 26, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.28→19.04 Å / Num. all: 46138 / Num. obs: 46008 / Rmerge(I) obs: 0.055 |
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Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.9_1692) / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.28→19.04 Å
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Refine LS restraints |
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LS refinement shell |
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