Mass: 18.015 Da / Num. of mol.: 162 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.72 Å3/Da / Density % sol: 54.82 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: protein concentration 15 mg mL-1 and a precipitant solution containing 30 % ammonium sulfate and 3.0 M sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution: 1.95→55.23 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.94 / SU B: 3.584 / SU ML: 0.1 / Cross valid method: THROUGHOUT / ESU R: 0.143 / ESU R Free: 0.145 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22389
536
4.8 %
RANDOM
Rwork
0.16725
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all
0.16986
10687
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obs
0.16986
10687
99.81 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK